FastQCFastQC Report
Tue 31 May 2016
SRR212892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180888
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12150.6716863473530583No Hit
ACGAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12040.665605236389368No Hit
ACGAGCGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT11320.6258016009906682No Hit
ACGAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8660.4787492813232498No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7620.42125514130290564No Hit
ACGAGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC7580.41904382822520014No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT6940.38366281898191146No Hit
ACGAGCGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC5980.3305913051169785No Hit
ACGAGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5100.28194241740745657No Hit
ACGAGCGGGAGAGTCCTGTAGTCGTGGGTGGTCTTATGGTTGTGTGTTCTGTCACC4910.2714386802883552No Hit
ACGAGCGGGGCTCCTCCCAGGTGACTGCACAGGATGTGCGGAAGGAAAGTCCATTG4550.2515368625890053No Hit
ACGAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3600.19901817699349875No Hit
ACGAGCGGGATTTGACTTAACTCCACAAGTTTGATTAAACTTGTGTAGAAAACCCC3350.18519747025783911No Hit
ACGAGCGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC3150.17414090486931139No Hit
ACGAGCGGGGCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCT3070.1697182787139003No Hit
ACGAGCGGGAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGCAGTGGAATTCA3060.16916545044447392No Hit
ACGAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.16529565255848924No Hit
ACGAGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2880.159214541594799No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2830.15645040024766704No Hit
ACGAGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.1442881783202866No Hit
ACGAGCGGGATAGCCAACACTATGACATAAAAACTTTGTGTTCAAAATGTACAATT2510.13875989562602273No Hit
ACGAGCGGGAGATATTTCAAGTAAGAATATTAAAATAGCAAGGAATGATGATTAAG2340.12936181504577418No Hit
ACGAGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2320.12825615850692143No Hit
ACGAGCGGGGGCCATCTTCCACAAGTACTCTGGCAAGGAAGGTGACAAGCACACCC2290.12659767369864225No Hit
ACGAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2290.12659767369864225No Hit
ACGAGCGGGGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGCC2260.1249391888903631No Hit
ACGAGCGGGAAGGAAGGTGACAAGCACACCCTGAGCAAGAAGGAGCTGAAGGAGTT2220.12272787581265754No Hit
ACGAGCGGGACTCTTTTTGTCCACTGGAGAGTGAGAGAACTGTTCACAGTTCATCT2220.12272787581265754No Hit
ACGAGCGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT2190.12106939100437838No Hit
ACGAGCGGGAACTAGAGATGTTCATGGTCAAAGTACTGCCTTTGCAGAGGAGCCTG2090.11554110831011453No Hit
ACGAGCGGGAGTGAGACATTTTACTTTTTAAATGATTTATCTTTTCCATTCAAATA2030.11222413869355623No Hit
ACGAGCGGGAGCCAGAGTGCCAATGGTGGGATCAGCAGGAAGACTGATGTTGGAGA2010.11111848215470346No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTACTCTTC1940.10724868426871877No Hit
ACGAGCGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGT1910.10559019946043961No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1910.10559019946043961No Hit
ACGAGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1880.10393171465216046No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1810.10006191676617576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGTA150.00824219450.2699449
CAATGCG204.0817627E-450.2279245
CCTTAGA150.00826949250.22791746
TTCACTA150.00828772850.19994443
AGCCGGA150.00832428950.1440938
CTCGTGT150.00832428950.1440939
GGTGTCG150.00832428950.1440938
AAGACGC402.593879E-950.1440939
GTGTCGC150.00832428950.1440939
TATCCTA204.1212136E-450.13014637
TACTCGT150.00833344850.13014237
AGAATAC150.00833344850.13014236
TACATTT150.00834261450.11620735
GTAGAGA1000.050.0605433
ACGTCCA150.0083885650.04664230
TACGTCC150.0083885650.04664229
GCATAGC150.0083885650.04664229
AGACGGG150.00839777250.03275328
ATACGTC150.00839777250.03275328
GTGTTCG150.00841621850.00499327
Produced by FastQC (version 0.11.5)