FastQCFastQC Report
Tue 31 May 2016
SRR212891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94062
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10631.130105674980332No Hit
TGCGGAGGGGCTTTTTCCTCCTCTTTAAGCCGTACCTGAACATCTCACACCATGGCCA10471.1130956177840148No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.6931598307499309No Hit
TGCGGAGGGATTTCAGACCATCTCATTCATGATAAGGGAAATTCAATCTAGAATTAAA5950.6325615019880504No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4400.4677765728987263No Hit
TGCGGAGGGATTTGACTTGGGGAGAATTATTTTTTACAAAAATACAAAATAGCTTTTT3780.4018626012629967No Hit
TGCGGAGGGGACGTTCTATTTATTTTCGCAGTAATCTCCATGTCCCACAGGCAGAGCT3250.3455167868001956No Hit
TGCGGAGGGGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGGAA2810.298739129510323No Hit
TGCGGAGGGAGTTTTAACAAATAAAGACAAAAAAAATCACATAAGATACTAAAAGTCT2540.2700346579915375No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.241330186472752No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.22644638642597437No Hit
TGCGGAGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG2040.21687822925304587No Hit
TGCGGAGGGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGA1840.1956156577576492No Hit
TGCGGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC1800.1913631434585699No Hit
TGCGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.1892368863090302No Hit
TGCGGAGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA1680.1786056005613319No Hit
TGCGGAGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA1670.17754247198656206No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.17647934341179222No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.16584805766409388No Hit
TGCGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCATCC1540.16372180051455423No Hit
TGCGGAGGGGAGGAGTTCACAGCTCAGATGATCACCATCACGCCGCCTGATCAAGATG1420.15096425761731624No Hit
TGCGGAGGGGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAA1410.1499011290425464No Hit
TGCGGAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1280.13608045757053858No Hit
TGCGGAGGGCTTGCGGAGGGGTGACAAAGTTGGATACCAAAAATAGCCAACACTATGA1280.13608045757053858No Hit
TGCGGAGGGATTAGGTGGAGTATAAATTCCACACTGGAAGGAAGCAGTGTGAAGGAGT1160.12332291467330059No Hit
TGCGGAGGGGGAGTAGTTTCAAATCAGGAAGGAAAAAACAAACAAAAAAGCTGTCGGA1150.12225978609853075No Hit
TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1130.1201335289489911No Hit
TGCGGAGGGAATGTGGGGATAGTGTGTGGAACACCCTGTTTCACCTTTATAGCCCCTG1130.1201335289489911No Hit
TGCGGAGGGGTGTTTCTCGGCCTCATCCTGTACTGCGTGGTGACATCTCCCATGCTGC1100.11694414322468158No Hit
TGCGGAGGGACCTTTGACAGCATGATTGTAGACAACACAACAATGCAGCTGGTATCCC1080.11481788607514193No Hit
TGCGGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.11162850035083244No Hit
TGCGGAGGGCCCTGGTGCCCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATG1030.10950224320129276No Hit
TGCGGAGGGATTTGGAACCTGGCCACAAACCGCCTCACGTTCCTCAACTCCTTCAAGA1020.10843911462652293No Hit
TGCGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.10737598605175311No Hit
TGCGGAGGGGCAGAAGCGTCTGGGACCTCGTGGACTGGTGGTGCTCGGTTTCCCGTGC970.10312347175267376No Hit
TGCGGAGGGATCAACAATCCAGACATCTCAATCCCCTTTCCTGATTGGCCATCCATTC970.10312347175267376No Hit
TGCGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.10312347175267376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCC203.413903E-452.05378349
TAGCCCC203.413903E-452.05378350
TATAGCC203.413903E-452.05378348
TGCTAAA203.413903E-452.05378347
AAGACAC150.007170249752.0537847
GCGGCCC150.007170249752.0537851
CTATTGA251.6334623E-552.0537852
ACGTTCC203.4228948E-452.0260837
TGGACCG203.4228948E-452.0260841
CACGTTC203.4228948E-452.0260836
TTACACC203.4228948E-452.0260835
GATACTA459.094947E-1152.02607745
TGATAAT150.0071853752.02607739
GATAATT150.0071853752.02607740
TCACATA459.094947E-1152.02607737
CAGCCGT150.0071853752.02607741
CACGCCG150.0071853752.02607739
ATATGTG150.0071853752.02607735
ATCACAT459.094947E-1152.02607736
CGCCTGA150.0071853752.02607744
Produced by FastQC (version 0.11.5)