SRR212890_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212890_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	94805
Sequences flagged as poor quality	0
Sequence length	58
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGCGGAGGGGCTTTTTCCTCCTCTTTAAGCCGTACCTGAACATCTCACACCATGGCCA	1072	1.1307420494699647	No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	973	1.0263171773640631	No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	649	0.6845630504720215	No Hit
TGCGGAGGGATTTCAGACCATCTCATTCATGATAAGGGAAATTCAATCTAGAATTAAA	602	0.6349876061389167	No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	425	0.44828859237381996	No Hit
TGCGGAGGGATTTGACTTGGGGAGAATTATTTTTTACAAAAATACAAAATAGCTTTTT	352	0.3712884341543167	No Hit
TGCGGAGGGGACGTTCTATTTATTTTCGCAGTAATCTCCATGTCCCACAGGCAGAGCT	293	0.30905542956595117	No Hit
TGCGGAGGGAGTTTTAACAAATAAAGACAAAAAAAATCACATAAGATACTAAAAGTCT	265	0.279521122303676	No Hit
TGCGGAGGGGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGGAA	259	0.2731923421760456	No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	219	0.2310004746585096	No Hit
TGCGGAGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG	217	0.22889088128263277	No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	206	0.21728811771531037	No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	194	0.20463055746004957	No Hit
TGCGGAGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA	190	0.200411370708296	No Hit
TGCGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.18880860714097358	No Hit
TGCGGAGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA	175	0.18458942038922	No Hit
TGCGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCATCC	169	0.17826064026158958	No Hit
TGCGGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC	163	0.1719318601339592	No Hit
TGCGGAGGGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGA	158	0.16665787669426718	No Hit
TGCGGAGGGGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAA	152	0.1603290965666368	No Hit
TGCGGAGGGGAGGAGTTCACAGCTCAGATGATCACCATCACGCCGCCTGATCAAGATG	129	0.1360687727440536	No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	128	0.13501397605611518	No Hit
TGCGGAGGGAATGTGGGGATAGTGTGTGGAACACCCTGTTTCACCTTTATAGCCCCTG	120	0.12657560255260797	No Hit
TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	118	0.12446600917673119	No Hit
TGCGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118	0.12446600917673119	No Hit
TGCGGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	113	0.11919202573703919	No Hit
TGCGGAGGGCTTGCGGAGGGGTGACAAAGTTGGATACCAAAAATAGCCAACACTATGA	113	0.11919202573703919	No Hit
TGCGGAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA	109	0.11497283898528557	No Hit
TGCGGAGGGGTGTTTCTCGGCCTCATCCTGTACTGCGTGGTGACATCTCCCATGCTGC	107	0.11286324560940879	No Hit
TGCGGAGGGATTTGGAACCTGGCCACAAACCGCCTCACGTTCCTCAACTCCTTCAAGA	106	0.11180844892147038	No Hit
TGCGGAGGGCCCTGGTGCCCCACTCTTCTGGTTCTTCAACATCTATGACCTTTTTATG	106	0.11180844892147038	No Hit
TGCGGAGGGAGCTCAGGCTCATCTCAACAGATGCCCAGGCTTTGAGAGCTTGTTATTT	104	0.1096988555455936	No Hit
TGCGGAGGGACCTTTGACAGCATGATTGTAGACAACACAACAATGCAGCTGGTATCCC	101	0.10653446548177839	No Hit
TGCGGAGGGCTTGACTTAATCTTAAATTTAAACTATTTTTTGCTATATAAACTTTAAC	98	0.1033700754179632	No Hit
TGCGGAGGGGGAGTAGTTTCAAATCAGGAAGGAAAAAACAAACAAAAAAGCTGTCGGA	97	0.10231527873002479	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGT	30	7.740273E-7	52.17002	24
CGTCGTG	30	7.740273E-7	52.17002	25
GAATTAT	40	1.8098945E-9	52.170017	24
CCCGAAT	20	3.3766121E-4	52.170017	26
AATCCCG	20	3.385459E-4	52.142403	23
TCATTCA	50	3.6379788E-12	52.142403	23
CCAATCC	20	3.385459E-4	52.142403	21
TCCGTCG	30	7.768649E-7	52.142403	23
TGGGACG	15	0.00712231	52.142403	22
TGTCCGT	30	7.768649E-7	52.142403	21
CTAAGTA	20	3.4121104E-4	52.059727	52
TCCATCA	15	0.007182178	52.032223	50
CTGTAAT	15	0.007182178	52.032223	49
ACTGTAG	15	0.007182178	52.032223	49
ATAGTCA	15	0.007182178	52.032223	49
TTAGGTT	15	0.007197203	52.004753	42
AGCAATC	15	0.007197203	52.004753	47
ATAGCCA	15	0.007197203	52.004753	43
AAGCGCC	15	0.007197203	52.004753	43
CAATGCT	15	0.007197203	52.004753	47

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content