FastQCFastQC Report
Tue 31 May 2016
SRR212889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94634
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGCTTTTTCCTCCTCTTTAAGCCGTACCTGAACATCTCACACCATGGCCA9881.0440222330240718No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9110.9626561278187543No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.6223978696874274No Hit
TGCGGAGGGATTTCAGACCATCTCATTCATGATAAGGGAAATTCAATCTAGAATTAAA4950.5230678191770399No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4060.42902128199167316No Hit
TGCGGAGGGATTTGACTTGGGGAGAATTATTTTTTACAAAAATACAAAATAGCTTTTT3300.34871187945136No Hit
TGCGGAGGGGACGTTCTATTTATTTTCGCAGTAATCTCCATGTCCCACAGGCAGAGCT3080.3254644208212693No Hit
TGCGGAGGGGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGGAA2690.2842530168861086No Hit
TGCGGAGGGAGTTTTAACAAATAAAGACAAAAAAAATCACATAAGATACTAAAAGTCT2320.24515501828095612No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.21556734366084074No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1890.1997168036857789No Hit
TGCGGAGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG1880.19866010102077475No Hit
TGCGGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATGGC1680.17752604772069236No Hit
TGCGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.1690724264006594No Hit
TGCGGAGGGGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAA1590.16801572373565526No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.1648456157406429No Hit
TGCGGAGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA1510.1595621024156223No Hit
TGCGGAGGGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTTGA1460.1542785890906017No Hit
TGCGGAGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA1420.1500517784305852No Hit
TGCGGAGGGCTTGCGGAGGGGTGACAAAGTTGGATACCAAAAATAGCCAACACTATGA1220.12891772513050279No Hit
TGCGGAGGGATTAGGTGGAGTATAAATTCCACACTGGAAGGAAGCAGTGTGAAGGAGT1210.12786102246549866No Hit
TGCGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCATCC1160.12257750914047806No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.12152080647547393No Hit
TGCGGAGGGGAGGAGTTCACAGCTCAGATGATCACCATCACGCCGCCTGATCAAGATG1110.11729399581545744No Hit
TGCGGAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1090.1151805904854492No Hit
TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1080.1141238878204451No Hit
TGCGGAGGGGCAGAAGCGTCTGGGACCTCGTGGACTGGTGGTGCTCGGTTTCCCGTGC1040.1098970771604286No Hit
TGCGGAGGGGTGTTTCTCGGCCTCATCCTGTACTGCGTGGTGACATCTCCCATGCTGC1040.1098970771604286No Hit
TGCGGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1000.10567026650041211No Hit
TGCGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.10355686117040387No Hit
TGCGGAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA960.10144345584039563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT203.3530215E-452.2438810
ATCCGTT203.3530215E-452.2438810
GCGGGTG203.3530215E-452.243889
GGCGGGT203.3530215E-452.243888
GGTGTAC251.5986005E-552.2438778
GACGTCT251.5986005E-552.2438779
GGACGTC251.5986005E-552.2438778
AGGGACC2100.052.2438776
GGGATAT251.5986005E-552.2438777
GTACCCC150.007067638452.2438749
ATTAGGT307.6646575E-752.24387410
GGCCCTC150.007067638452.2438749
GGGACTA150.007067638452.2438747
GTGTACG150.007067638452.2438749
GCAATTC150.007067638452.2438749
GGATTAG307.6646575E-752.2438748
TGTACGC150.007067638452.24387410
GATTAGG307.6646575E-752.2438749
AGCCGTA1050.052.1328428
AAGCCGT1050.052.1328427
Produced by FastQC (version 0.11.5)