FastQCFastQC Report
Tue 31 May 2016
SRR212888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94759
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGCTTTTTCCTCCTCTTTAAGCCGTACCTGAACATCTCACACCATGGC11931.2589833155689698No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10551.11335071075043No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.6891166010616406No Hit
TGCGGAGGGATTTCAGACCATCTCATTCATGATAAGGGAAATTCAATCTAGAATTA5920.6247427684969238No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4520.47699954621724583No Hit
TGCGGAGGGATTTGACTTGGGGAGAATTATTTTTTACAAAAATACAAAATAGCTTT3070.3239797802847223No Hit
TGCGGAGGGGACGTTCTATTTATTTTCGCAGTAATCTCCATGTCCCACAGGCAGAG2800.29548644455935585No Hit
TGCGGAGGGGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGG2610.2754355786785424No Hit
TGCGGAGGGAGTTTTAACAAATAAAGACAAAAAAAATCACATAAGATACTAAAAGT2590.2733249612174042No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.21633828976667124No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.20578520246097998No Hit
TGCGGAGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA1820.1920661889635813No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.1889002627718739No Hit
TGCGGAGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTC1780.18784495404130477No Hit
TGCGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.17940248419675175No Hit
TGCGGAGGGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTT1600.1688493968910605No Hit
TGCGGAGGGAATGTGGGGATAGTGTGTGGAACACCCTGTTTCACCTTTATAGCCCC1450.15301976593252356No Hit
TGCGGAGGGGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAAC1440.15196445720195442No Hit
TGCGGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG1360.1435219873574014No Hit
TGCGGAGGGACCTTTGACAGCATGATTGTAGACAACACAACAATGCAGCTGGTATC1350.14246667862683227No Hit
TGCGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCAT1340.14141136989626316No Hit
TGCGGAGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTC1330.14035606116569402No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.1308582825905719No Hit
TGCGGAGGGATCAACAATCCAGACATCTCAATCCCCTTTCCTGATTGGCCATCCAT1230.12980297386000275No Hit
TGCGGAGGGCTGGGAGGTACCTGGGCTGCCCAAGGCCCATGGACATTGATTCACCT1210.1276923563988645No Hit
TGCGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.12452643020715709No Hit
TGCGGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.1213605040154497No Hit
TGCGGAGGGGTCTCCATGTTTTCTTTTTTAACTTTGCACTTTCTTTGTATAATAAT1110.1171392690931732No Hit
TGCGGAGGGCTTGCGGAGGGGTGACAAAGTTGGATACCAAAAATAGCCAACACTAT1050.11080741670975844No Hit
TGCGGAGGGGGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTGAGAAGC1040.10975210797918931No Hit
TGCGGAGGGGAGGAGTTCACAGCTCAGATGATCACCATCACGCCGCCTGATCAAGA1000.1055308730569128No Hit
TGCGGAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC990.10447556432634367No Hit
TGCGGAGGGGTGTTTCTCGGCCTCATCCTGTACTGCGTGGTGACATCTCCCATGCT970.10236494686520542No Hit
TGCGGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG970.10236494686520542No Hit
TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA970.10236494686520542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGT150.00827304950.20382350
AGGATAA204.0814618E-450.2038250
CTAGAAT750.050.1771948
CAGGCAC150.00829043250.17718549
CACAGCG150.00829043250.17718549
CTATATA355.1193638E-850.1240143
GCTATAT355.1193638E-850.1240142
TTCAATC750.050.1240142
GCGATAG252.0426514E-550.1240142
CGATAGC252.0426514E-550.1240143
GCCCAAA150.0083252850.12400445
AGATACT301.0200147E-650.12400444
ACTTGGA204.1136617E-450.12400443
TTGAGAG150.0083252850.12400442
GTCCCAC204.1136617E-450.12400442
GCCAAAC150.0083252850.12400444
ATTTACT204.12444E-450.09745839
ACTTAGC204.12444E-450.09745839
CTTAGCA204.12444E-450.09745840
CATAAAC252.06196E-550.04444534