FastQCFastQC Report
Tue 31 May 2016
SRR212887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84487
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGCTTTTTCCTCCTCTTTAAGCCGTACCTGAACATCTCACACCATGGC12191.4428255234533123No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9001.0652526424183602No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.6959650597133287No Hit
TGCGGAGGGATTTCAGACCATCTCATTCATGATAAGGGAAATTCAATCTAGAATTA4380.518422952643602No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3770.44622249576857975No Hit
TGCGGAGGGATTTGACTTGGGGAGAATTATTTTTTACAAAAATACAAAATAGCTTT2680.317208564631245No Hit
TGCGGAGGGGACGTTCTATTTATTTTCGCAGTAATCTCCATGTCCCACAGGCAGAG2370.2805165291701682No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.2603950903689325No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.22725389704925017No Hit
TGCGGAGGGGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGG1790.21186691443654052No Hit
TGCGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.1976635458709624No Hit
TGCGGAGGGAGTTTTAACAAATAAAGACAAAAAAAATCACATAAGATACTAAAAGT1660.19647993182383086No Hit
TGCGGAGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA1610.19056186158817331No Hit
TGCGGAGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTC1610.19056186158817331No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.17872572111685822No Hit
TGCGGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG1440.17044042278693763No Hit
TGCGGAGGGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTT1400.1657059665984116No Hit
TGCGGAGGGCTGGGAGGTACCTGGGCTGCCCAAGGCCCATGGACATTGATTCACCT1360.16097151040988555No Hit
TGCGGAGGGGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAAC1340.1586042823156225No Hit
TGCGGAGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1280.15150259803283345No Hit
TGCGGAGGGGGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTGAGAAGC1250.1479517558914389No Hit
TGCGGAGGGAATGTGGGGATAGTGTGTGGAACACCCTGTTTCACCTTTATAGCCCC1180.13966645756151835No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.13848284351438683No Hit
TGCGGAGGGATCAACAATCCAGACATCTCAATCCCCTTTCCTGATTGGCCATCCAT1120.1325647732787293No Hit
TGCGGAGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTC1090.12901393113733473No Hit
TGCGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCAT1080.12783031709020323No Hit
TGCGGAGGGACCTTTGACAGCATGATTGTAGACAACACAACAATGCAGCTGGTATC1070.1266467030430717No Hit
TGCGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.1242794749488087No Hit
TGCGGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1020.12072863280741415No Hit
TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1010.11954501876028266No Hit
TGCGGAGGGCTTGACTTAATCTTAAATTTAAACTATTTTTTGCTATATAAACTTTA990.11717779066601963No Hit
TGCGGAGGGCTTGCGGAGGGGTGACAAAGTTGGATACCAAAAATAGCCAACACTAT960.11362694852462508No Hit
TGCGGAGGGAGCTCAGGCTCATCTCAACAGATGCCCAGGCTTTGAGAGCTTGTTAT960.11362694852462508No Hit
TGCGGAGGGATTAGGTGGAGTATAAATTCCACACTGGAAGGAAGCAGTGTGAAGGA960.11362694852462508No Hit
TGCGGAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC920.10889249233609906No Hit
TGCGGAGGGACTTTAGGACAGCAGGAGTTGTCACCTCGAACTGCCTTTAAAACTGC910.10770887828896752No Hit
TGCGGAGGGCAAGTTTTTTACCATTACCAATTACACTTGCTTTGCCTTATTTTGAT900.10652526424183603No Hit
TGCGGAGGGGTGTTTCTCGGCCTCATCCTGTACTGCGTGGTGACATCTCCCATGCT890.10534165019470451No Hit
TGCGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.10534165019470451No Hit
TGCGGAGGGGATCAGTCATGGCATGCCCTCTGGATCAGGCCATTGGCCTTCTCGTG870.10297442210044148No Hit
TGCGGAGGGCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCAGATTG850.10060719400617846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGC150.00817140650.35607550
AACGCGG150.00817140650.35607550
CGCAATT150.00817140650.35607550
TAGCTTT309.866599E-750.35607550
CTAGAAT505.456968E-1250.3259648
GTCGCGT150.00819076850.3259644
TCGTTCC204.0301925E-450.3259646
TTCAATC451.2551027E-1050.3259642
CTAACCG150.00819076850.3259645
ACCTAAC150.00819076850.3259643
AGCGCGA150.00819076850.3259647
ACTCGTT204.0301925E-450.3259644
GCGTGCA150.00819076850.3259647
CGACTGA150.00819076850.3259645
CGACTCG204.0301925E-450.3259642
CTGATAC150.00819076850.3259649
TAAGATA251.9926687E-550.3259642
CCTGATA150.00819076850.3259648
GACTCGT204.0301925E-450.3259643
CAAGTAG150.008210164550.29587641