SRR212887_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212887_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84487
Sequences flagged as poor quality	0
Sequence length	56
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGCGGAGGGGCTTTTTCCTCCTCTTTAAGCCGTACCTGAACATCTCACACCATGGC	1219	1.4428255234533123	No Hit
TGCGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	900	1.0652526424183602	No Hit
TGCGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	588	0.6959650597133287	No Hit
TGCGGAGGGATTTCAGACCATCTCATTCATGATAAGGGAAATTCAATCTAGAATTA	438	0.518422952643602	No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	377	0.44622249576857975	No Hit
TGCGGAGGGATTTGACTTGGGGAGAATTATTTTTTACAAAAATACAAAATAGCTTT	268	0.317208564631245	No Hit
TGCGGAGGGGACGTTCTATTTATTTTCGCAGTAATCTCCATGTCCCACAGGCAGAG	237	0.2805165291701682	No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	220	0.2603950903689325	No Hit
TGCGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	192	0.22725389704925017	No Hit
TGCGGAGGGGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGG	179	0.21186691443654052	No Hit
TGCGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	167	0.1976635458709624	No Hit
TGCGGAGGGAGTTTTAACAAATAAAGACAAAAAAAATCACATAAGATACTAAAAGT	166	0.19647993182383086	No Hit
TGCGGAGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA	161	0.19056186158817331	No Hit
TGCGGAGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTC	161	0.19056186158817331	No Hit
TGCGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151	0.17872572111685822	No Hit
TGCGGAGGGACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAGGACATG	144	0.17044042278693763	No Hit
TGCGGAGGGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTACCCAAGACCTT	140	0.1657059665984116	No Hit
TGCGGAGGGCTGGGAGGTACCTGGGCTGCCCAAGGCCCATGGACATTGATTCACCT	136	0.16097151040988555	No Hit
TGCGGAGGGGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAAC	134	0.1586042823156225	No Hit
TGCGGAGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	128	0.15150259803283345	No Hit
TGCGGAGGGGGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTGAGAAGC	125	0.1479517558914389	No Hit
TGCGGAGGGAATGTGGGGATAGTGTGTGGAACACCCTGTTTCACCTTTATAGCCCC	118	0.13966645756151835	No Hit
TGCGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.13848284351438683	No Hit
TGCGGAGGGATCAACAATCCAGACATCTCAATCCCCTTTCCTGATTGGCCATCCAT	112	0.1325647732787293	No Hit
TGCGGAGGGACGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTC	109	0.12901393113733473	No Hit
TGCGGAGGGGGACTTGATTGCTGCTGCTTCTGAGCTTGCTCCCGCGCTTTCGGCAT	108	0.12783031709020323	No Hit
TGCGGAGGGACCTTTGACAGCATGATTGTAGACAACACAACAATGCAGCTGGTATC	107	0.1266467030430717	No Hit
TGCGGAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.1242794749488087	No Hit
TGCGGAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	102	0.12072863280741415	No Hit
TGCGGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	101	0.11954501876028266	No Hit
TGCGGAGGGCTTGACTTAATCTTAAATTTAAACTATTTTTTGCTATATAAACTTTA	99	0.11717779066601963	No Hit
TGCGGAGGGCTTGCGGAGGGGTGACAAAGTTGGATACCAAAAATAGCCAACACTAT	96	0.11362694852462508	No Hit
TGCGGAGGGAGCTCAGGCTCATCTCAACAGATGCCCAGGCTTTGAGAGCTTGTTAT	96	0.11362694852462508	No Hit
TGCGGAGGGATTAGGTGGAGTATAAATTCCACACTGGAAGGAAGCAGTGTGAAGGA	96	0.11362694852462508	No Hit
TGCGGAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	92	0.10889249233609906	No Hit
TGCGGAGGGACTTTAGGACAGCAGGAGTTGTCACCTCGAACTGCCTTTAAAACTGC	91	0.10770887828896752	No Hit
TGCGGAGGGCAAGTTTTTTACCATTACCAATTACACTTGCTTTGCCTTATTTTGAT	90	0.10652526424183603	No Hit
TGCGGAGGGGTGTTTCTCGGCCTCATCCTGTACTGCGTGGTGACATCTCCCATGCT	89	0.10534165019470451	No Hit
TGCGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.10534165019470451	No Hit
TGCGGAGGGGATCAGTCATGGCATGCCCTCTGGATCAGGCCATTGGCCTTCTCGTG	87	0.10297442210044148	No Hit
TGCGGAGGGCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAATTCAGATTG	85	0.10060719400617846	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATAGC	15	0.008171406	50.356075	50
AACGCGG	15	0.008171406	50.356075	50
CGCAATT	15	0.008171406	50.356075	50
TAGCTTT	30	9.866599E-7	50.356075	50
CTAGAAT	50	5.456968E-12	50.32596	48
GTCGCGT	15	0.008190768	50.32596	44
TCGTTCC	20	4.0301925E-4	50.32596	46
TTCAATC	45	1.2551027E-10	50.32596	42
CTAACCG	15	0.008190768	50.32596	45
ACCTAAC	15	0.008190768	50.32596	43
AGCGCGA	15	0.008190768	50.32596	47
ACTCGTT	20	4.0301925E-4	50.32596	44
GCGTGCA	15	0.008190768	50.32596	47
CGACTGA	15	0.008190768	50.32596	45
CGACTCG	20	4.0301925E-4	50.32596	42
CTGATAC	15	0.008190768	50.32596	49
TAAGATA	25	1.9926687E-5	50.32596	42
CCTGATA	15	0.008190768	50.32596	48
GACTCGT	20	4.0301925E-4	50.32596	43
CAAGTAG	15	0.0082101645	50.295876	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content