SRR212886_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212886_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61359
Sequences flagged as poor quality	0
Sequence length	58
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	531	0.8653987190143255	No Hit
TGTCACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	352	0.573672973809873	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	262	0.42699522482439417	No Hit
TGTCACGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTT	260	0.4237357192913835	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	236	0.3846216528952558	No Hit
TGTCACGGGGGTTGAGGACTTCTATCCAATCCTGATACTTCTGTCAAAAACCTCACCC	204	0.3324695643670855	No Hit
TGTCACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	195	0.3178017894685376	No Hit
TGTCACGGGCATCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTAT	169	0.2754282175393993	No Hit
TGTCACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	166	0.2705389592398833	No Hit
TGTCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	148	0.2412034094427875	No Hit
TGTCACGGGATAGCCATATTAGTTCACCCAAGAAAGGACTGGATGTAGGTGACGAACA	143	0.23305464561026093	No Hit
TGTCACGGGAGCAAATGCACTGAAACGGAAACGTGAAGACGATGATGATGACGATGAT	140	0.22816538731074495	No Hit
TGTCACGGGCAAGCTTCTAGGTTACCCAGCAAGCCCTGGAAACCAGAGCAGCTGTCTA	127	0.2069786013461758	No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	126	0.20534884857967045	No Hit
TGTCACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.2004595902801545	No Hit
TGTCACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG	116	0.18905132091461724	No Hit
TGTCACGGGAGCTATGTAACCTTGATGCTCTGTAAGGTGATATTTGTATATAGCAACC	109	0.17764305154908	No Hit
TGTCACGGGGAGTTTTGCAATTCTTCTATCAAATTTTGCCATTTGTTATGCTTATAAA	108	0.17601329878257468	No Hit
TGTCACGGGGTATATGTTGATGTTGAAGACTTCAGTTATTTGATGTCACTGACAAATT	108	0.17601329878257468	No Hit
TGTCACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	94	0.15319676005150018	No Hit
TGTCACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	94	0.15319676005150018	No Hit
TGTCACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	93	0.15156700728499486	No Hit
TGTCACGGGAGACAGATTTCTGAAATGCCTCTGGTCATTTAAATAGTGAACTTGATCA	93	0.15156700728499486	No Hit
TGTCACGGGGGCCATCTTCCTGCCCTGAGCTGTGCCCTGTGTCTAGGGAAAGGGAGTT	90	0.1466777489854789	No Hit
TGTCACGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA	90	0.1466777489854789	No Hit
TGTCACGGGATCTGTAAACTGTTCAGTTTAAAATACTGTAGATAATTAACCAAGGTAG	88	0.14341824345246826	No Hit
TGTCACGGGGAATTTGTTGGTTTGAAACATTGAAAAATTTTCATTAGACAATGTTTAC	85	0.13852898515295228	No Hit
TGTCACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	83	0.13526947961994165	No Hit
TGTCACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	83	0.13526947961994165	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	79	0.12875046855392036	No Hit
TGTCACGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG	78	0.12712071578741505	No Hit
TGTCACGGGGTTTTGTTTTGTTTTTTTTCCTGAAGGTGCTATTTAACAAGGGAGAAGA	71	0.11571244642187781	No Hit
TGTCACGGGATGCCCAGAAGAGCTATTGCTGGATCCTCCAGTAGTACTATGTCCAATT	70	0.11408269365537248	No Hit
TGTCACGGGGTGTAACATTGGTGAAGGATCCAGGAATATGACAGAAAGGTGGAATAAA	69	0.11245294088886716	No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC	68	0.11082318812236185	No Hit
TGTCACGGGGGGAGCTACCCAGCTTCCTGTGGTACCAAGGAGTTATTTTGGATGATTA	68	0.11082318812236185	No Hit
TGTCACGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	67	0.10919343535585652	No Hit
TGTCACGGGAGTGCGGCAAAAGCATGTCTCATTAAATGCCTCATCTGTACAGTATAGC	66	0.1075636825893512	No Hit
TGTCACGGGACCCTAGTTGCCTGGAAGTCAGTCTTCAACTAGCAGCCTTTGGATGAAG	64	0.10430417705634056	No Hit
TGTCACGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64	0.10430417705634056	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATGTA	20	3.391504E-4	52.094036	41
GGTGACG	20	3.391504E-4	52.094036	48
CTAGGGA	20	3.391504E-4	52.094036	43
ACGTTGC	15	0.0071364986	52.094032	48
CGTGCAG	25	1.6192751E-5	52.094032	48
CCCTAGA	15	0.0071364986	52.094032	48
CGTTGCT	15	0.0071364986	52.094032	49
ATAGCAA	25	1.6192751E-5	52.094032	50
GTAGGTG	25	1.6192751E-5	52.094032	45
TAACGTT	15	0.0071364986	52.094032	46
TAGCAAC	25	1.6192751E-5	52.094032	51
ATATAGC	25	1.6192751E-5	52.094032	48
CCCCCAC	15	0.0071364986	52.094032	41
TATAGCA	25	1.6192751E-5	52.094032	49
AGCCTAT	25	1.6192751E-5	52.094032	52
CTTTCAT	15	0.0071364986	52.094032	52
ACTCCTA	15	0.0071364986	52.094032	46
GCGTGCA	25	1.6192751E-5	52.094032	47
GCAGCCC	25	1.6192751E-5	52.094032	51
CGCGTGC	30	7.7727964E-7	52.094032	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content