FastQCFastQC Report
Tue 31 May 2016
SRR212885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65389
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5780.8839407239749806No Hit
TGTCACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3830.5857254278242517No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2880.4404410527764609No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2730.41750141461102025No Hit
TGTCACGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTT2500.3823273027573445No Hit
TGTCACGGGGGTTGAGGACTTCTATCCAATCCTGATACTTCTGTCAAAAACCTCACCC1930.29515667772866994No Hit
TGTCACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1880.2875101316735231No Hit
TGTCACGGGCATCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTAT1790.2737463487742587No Hit
TGTCACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.24927740139778862No Hit
TGTCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.24163085534264173No Hit
TGTCACGGGAGCAAATGCACTGAAACGGAAACGTGAAGACGATGATGATGACGATGAT1570.24010154613161236No Hit
TGTCACGGGATAGCCATATTAGTTCACCCAAGAAAGGACTGGATGTAGGTGACGAACA1450.22174983559925981No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1330.2033981250669073No Hit
TGTCACGGGCAAGCTTCTAGGTTACCCAGCAAGCCCTGGAAACCAGAGCAGCTGTCTA1310.20033950664484854No Hit
TGTCACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG1300.1988101974338191No Hit
TGTCACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.1988101974338191No Hit
TGTCACGGGGAGTTTTGCAATTCTTCTATCAAATTTTGCCATTTGTTATGCTTATAAA1140.1743412500573491No Hit
TGTCACGGGAGCTATGTAACCTTGATGCTCTGTAAGGTGATATTTGTATATAGCAACC1130.17281194084631973No Hit
TGTCACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1070.16363608558014345No Hit
TGTCACGGGAGACAGATTTCTGAAATGCCTCTGGTCATTTAAATAGTGAACTTGATCA1040.15904815794705532No Hit
TGTCACGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1000.1529309211029378No Hit
TGTCACGGGGTATATGTTGATGTTGAAGACTTCAGTTATTTGATGTCACTGACAAATT1000.1529309211029378No Hit
TGTCACGGGATCTGTAAACTGTTCAGTTTAAAATACTGTAGATAATTAACCAAGGTAG960.1468136842588203No Hit
TGTCACGGGGGCCATCTTCCTGCCCTGAGCTGTGCCCTGTGTCTAGGGAAAGGGAGTT960.1468136842588203No Hit
TGTCACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA950.1452843750477909No Hit
TGTCACGGGGAATTTGTTGGTTTGAAACATTGAAAAATTTTCATTAGACAATGTTTAC940.14375506583676154No Hit
TGTCACGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC850.12999128293749712No Hit
TGTCACGGGGTGTGGGCTGCGCATTCTCTTCCGGATGTACATGGACGAGAGCAGAGTC770.11775680924926211No Hit
TGTCACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT760.11622750003823273No Hit
TGTCACGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCCTCTGCTCTTCCAG740.11316888161617399No Hit
TGTCACGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA730.1116395724051446No Hit
TGTCACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA730.1116395724051446No Hit
TGTCACGGGAGTGCGGCAAAAGCATGTCTCATTAAATGCCTCATCTGTACAGTATAGC710.10858095398308584No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG710.10858095398308584No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC690.1055223355610271No Hit
TGTCACGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG660.10093440792793897No Hit
TGTCACGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG660.10093440792793897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA150.007165568452.04444545
AAATCGC150.007165568452.04444551
TCATTAG307.832241E-752.04444541
ATTACAA150.007165568452.04444545
ATGTAGG150.007165568452.04444543
GGCCAAT150.007165568452.04444545
GCTTATA150.007165568452.04444550
AAACCAG203.409185E-452.04444540
TATTACA150.007165568452.04444544
CTCGCCA150.007165568452.04444544
TCCAAAG203.409185E-452.04444552
GTATAGC150.007165568452.04444552
AATAGTG251.629646E-552.04444542
CAACTGC150.007165568452.04444544
CCCGGGG150.007165568452.04444549
CATTTAT150.007165568452.04444551
AAAATCG150.007165568452.04444550
TGATGAC203.409185E-452.04444546
GCCAATG150.007165568452.04444546
AATCGCT150.007165568452.04444552
Produced by FastQC (version 0.11.5)