FastQCFastQC Report
Tue 31 May 2016
SRR212884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67698
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.7991373452686933No Hit
TGTCACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.5819965139295105No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2760.4076929894531596No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2670.3943986528405566No Hit
TGTCACGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTT2510.3707642766403734No Hit
TGTCACGGGGGTTGAGGACTTCTATCCAATCCTGATACTTCTGTCAAAAACCTCACCC1880.2777039203521522No Hit
TGTCACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1840.2717953263021064No Hit
TGTCACGGGCATCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCTAT1530.2260037224142515No Hit
TGTCACGGGAGCAAATGCACTGAAACGGAAACGTGAAGACGATGATGATGACGATGAT1500.22157227687671718No Hit
TGTCACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.2156636828266714No Hit
TGTCACGGGCAAGCTTCTAGGTTACCCAGCAAGCCCTGGAAACCAGAGCAGCTGTCTA1360.2008921977015569No Hit
TGTCACGGGATAGCCATATTAGTTCACCCAAGAAAGGACTGGATGTAGGTGACGAACA1360.2008921977015569No Hit
TGTCACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.18907500960146534No Hit
TGTCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.18907500960146534No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1220.18021211852639663No Hit
TGTCACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCG1150.1698720789388165No Hit
TGTCACGGGGAGTTTTGCAATTCTTCTATCAAATTTTGCCATTTGTTATGCTTATAAA1110.16396348488877072No Hit
TGTCACGGGGTATATGTTGATGTTGAAGACTTCAGTTATTTGATGTCACTGACAAATT1020.15066914827616767No Hit
TGTCACGGGAGCTATGTAACCTTGATGCTCTGTAAGGTGATATTTGTATATAGCAACC970.14328340571361045No Hit
TGTCACGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA960.141806257201099No Hit
TGTCACGGGGGCCATCTTCCTGCCCTGAGCTGTGCCCTGTGTCTAGGGAAAGGGAGTT920.1358976631510532No Hit
TGTCACGGGATCTGTAAACTGTTCAGTTTAAAATACTGTAGATAATTAACCAAGGTAG910.13442051463854177No Hit
TGTCACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT870.12851192058849598No Hit
TGTCACGGGAGACAGATTTCTGAAATGCCTCTGGTCATTTAAATAGTGAACTTGATCA870.12851192058849598No Hit
TGTCACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT850.12555762356347308No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG760.11226328695087004No Hit
TGTCACGGGGAATTTGTTGGTTTGAAACATTGAAAAATTTTCATTAGACAATGTTTAC740.10930898992584714No Hit
TGTCACGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA690.1019232473632899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG150.00694466952.4595836
CGGGTCC150.00694466952.4595836
GGGTATA150.00694466952.4595838
GGGTAAT150.00694466952.4595838
GGATCGT150.00694466952.4595838
GGATAGT150.00694466952.4595838
ACGGGTC850.052.4595835
CGGGATC2750.052.4595836
GCGTGAA150.00694466952.45958310
ATCGCTC150.00694466952.45958310
GGGGACG251.5551926E-552.459587
GGATGCA203.278629E-452.459588
GGGTATT251.5551926E-552.459588
CGGGGTC2000.052.459586
CGGGCGA251.5551926E-552.459586
GGATTCC203.278629E-452.459588
GATTCGT401.70985E-952.459589
ACGGGAT8000.052.1317065
ATCTCTT203.401757E-452.0698452
ACAAATT203.401757E-452.0698452
Produced by FastQC (version 0.11.5)