FastQCFastQC Report
Tue 31 May 2016
SRR212883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95688
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7520.7858874676030433No Hit
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6510.6803360922999749No Hit
TGTCACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.5570186439260931No Hit
TGTCACGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC3600.3762227238525207No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2880.3009781790820165No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2410.2518602123568264No Hit
TGTCACGGGCATCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCT2370.24767995986957614No Hit
TGTCACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2360.24663489674776354No Hit
TGTCACGGGAGACAGATTTCTGAAATGCCTCTGGTCATTTAAATAGTGAACTTGAT2210.23095894992057522No Hit
TGTCACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.22991388679876262No Hit
TGTCACGGGATAGCCATATTAGTTCACCCAAGAAAGGACTGGATGTAGGTGACGAA2190.22886882367695008No Hit
TGTCACGGGAGCAAATGCACTGAAACGGAAACGTGAAGACGATGATGATGACGATG2150.22468857118969984No Hit
TGTCACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2050.2142379399715743No Hit
TGTCACGGGGGTTGAGGACTTCTATCCAATCCTGATACTTCTGTCAAAAACCTCAC1880.19647186690076082No Hit
TGTCACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.17766073070813476No Hit
TGTCACGGGGTGGCCATCTTCCACAAGTACTCTGGCAAGGAAGGTGACAAGCACAC1690.17661566758632222No Hit
TGTCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.17243541509907198No Hit
TGTCACGGGGTATATGTTGATGTTGAAGACTTCAGTTATTTGATGTCACTGACAAA1550.16198478388094642No Hit
TGTCACGGGCAAGCTTCTAGGTTACCCAGCAAGCCCTGGAAACCAGAGCAGCTGTC1530.1598946576373213No Hit
TGTCACGGGGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGC1510.15780453139369618No Hit
TGTCACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1500.1567594682718836No Hit
TGTCACGGGGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAA1440.15048908954100826No Hit
TGTCACGGGGACTACCCTCGGGAGTACCAGTATGGCGACGACCAGGGCATTGATTA1430.1494440264191957No Hit
TGTCACGGGAGCTATGTAACCTTGATGCTCTGTAAGGTGATATTTGTATATAGCAA1320.13794833207925758No Hit
TGTCACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1250.13063289022656968No Hit
TGTCACGGGGGCCATCTTCCTGCCCTGAGCTGTGCCCTGTGTCTAGGGAAAGGGAG1220.127497700861132No Hit
TGTCACGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1200.1254075746175069No Hit
TGTCACGGGGTATTTTAATGGGGTTTGTCCTCATCCACAAATAATTATTTTTTTGT1180.12331744837388177No Hit
TGTCACGGGGAGTTTTGCAATTCTTCTATCAAATTTTGCCATTTGTTATGCTTATA1180.12331744837388177No Hit
TGTCACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1150.1201822590084441No Hit
TGTCACGGGGTACTTAATCGGCATACCCTTTTGTCCCAAATGCACCCCGTCGACAT1140.11913719588663155No Hit
TGTCACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1110.11600200652119388No Hit
TGTCACGGGAGCAACCAGGGAGATTTCTCCATTTTCCTCTTGTCTACAGTGCGGCT1100.11495694339938131No Hit
TGTCACGGGGAATTTGTTGGTTTGAAACATTGAAAAATTTTCATTAGACAATGTTT1080.1128668171557562No Hit
TGTCACGGGATCTTTCTTAGAACAAATGCAGTTCAATTGCATCCCATTTCCAGTCA1070.11182175403394365No Hit
TGTCACGGGGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGA1050.10973162779031853No Hit
TGTCACGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAA1030.10764150154669343No Hit
TGTCACGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCCTCTGCTCTTCC1030.10764150154669343No Hit
TGTCACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG1020.10659643842488085No Hit
TGTCACGGGGTGTAACATTGGTGAAGGATCCAGGAATATGACAGAAAGGTGGAATA1020.10659643842488085No Hit
TGTCACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT970.10137112281581807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAT150.00825878950.2260350
GGAATAG150.00825878950.2260349
GCCTTAA150.00825878950.2260348
GCGACAC150.00825878950.2260350
TTAATCA150.00825878950.2260350
GTTTCCC150.00825878950.2260349
CACGACT150.00825878950.2260349
CGCGACA150.00825878950.2260349
CCATGTA150.00825878950.2260350
GGACGCG252.0183219E-550.2260350
TATAGCA150.00825878950.2260349
CCGCGAC150.00825878950.2260348
AAACCTC402.5356712E-950.22602548
CAGTGCG204.083329E-450.1995847
ACCGCGA150.00827601450.19957747
TACACGA150.00827601450.19957747
TATCATG150.00831054450.14676736
ATGGGAT150.00831054450.14676740
CTCACTC150.00831054450.14676744
CTCACCG150.00831054450.14676744
Produced by FastQC (version 0.11.5)