FastQCFastQC Report
Tue 31 May 2016
SRR212882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89693
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6930.7726355456947588No Hit
TGTCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6750.7525670899624274No Hit
TGTCACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.518435106418561No Hit
TGTCACGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC3250.3623471173893169No Hit
TGTCACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3010.33558917641287506No Hit
TGTCACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2580.28764786549675003No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2340.2608899245203082No Hit
TGTCACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2280.25420043927619773No Hit
TGTCACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.25420043927619773No Hit
TGTCACGGGGTGGCCATCTTCCACAAGTACTCTGGCAAGGAAGGTGACAAGCACAC1980.22075301305564538No Hit
TGTCACGGGAGACAGATTTCTGAAATGCCTCTGGTCATTTAAATAGTGAACTTGAT1820.20291438573801746No Hit
TGTCACGGGCATCTCCGAAAACTATATCTCAGACCCAACTATCCTGTGGAGAGCCT1730.19288015787185175No Hit
TGTCACGGGAGCAAATGCACTGAAACGGAAACGTGAAGACGATGATGATGACGATG1730.19288015787185175No Hit
TGTCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.18730558683509305No Hit
TGTCACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.18284593000568605No Hit
TGTCACGGGATAGCCATATTAGTTCACCCAAGAAAGGACTGGATGTAGGTGACGAA1630.1817310157983343No Hit
TGTCACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1620.18061610159098257No Hit
TGTCACGGGGACTACCCTCGGGAGTACCAGTATGGCGACGACCAGGGCATTGATTA1620.18061610159098257No Hit
TGTCACGGGGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAA1510.16835204531011339No Hit
TGTCACGGGCAAGCTTCTAGGTTACCCAGCAAGCCCTGGAAACCAGAGCAGCTGTC1450.16166256006600294No Hit
TGTCACGGGGGTTGAGGACTTCTATCCAATCCTGATACTTCTGTCAAAAACCTCAC1450.16166256006600294No Hit
TGTCACGGGGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGC1330.14828358957778198No Hit
TGTCACGGGGTATATGTTGATGTTGAAGACTTCAGTTATTTGATGTCACTGACAAA1290.14382393274837502No Hit
TGTCACGGGGAGTTTTGCAATTCTTCTATCAAATTTTGCCATTTGTTATGCTTATA1220.1360195332969128No Hit
TGTCACGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1210.13490461908956106No Hit
TGTCACGGGGTATTTTAATGGGGTTTGTCCTCATCCACAAATAATTATTTTTTTGT1180.1315598764675058No Hit
TGTCACGGGAGCTATGTAACCTTGATGCTCTGTAAGGTGATATTTGTATATAGCAA1130.1259853054307471No Hit
TGTCACGGGGGCCATCTTCCTGCCCTGAGCTGTGCCCTGTGTCTAGGGAAAGGGAG1120.12487039122339536No Hit
TGTCACGGGGTGTAACATTGGTGAAGGATCCAGGAATATGACAGAAAGGTGGAATA1110.12375547701604361No Hit
TGTCACGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG1080.12041073439398838No Hit
TGTCACGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC1060.11818090597928489No Hit
TGTCACGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1040.1159510775645814No Hit
TGTCACGGGATCTGTAAACTGTTCAGTTTAAAATACTGTAGATAATTAACCAAGGT1020.11372124914987793No Hit
TGTCACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1020.11372124914987793No Hit
TGTCACGGGGTACTTAATCGGCATACCCTTTTGTCCCAAATGCACCCCGTCGACAT1020.11372124914987793No Hit
TGTCACGGGAGCAACCAGGGAGATTTCTCCATTTTCCTCTTGTCTACAGTGCGGCT1020.11372124914987793No Hit
TGTCACGGGATTTAATGTAAGTACACTGTAGCTGTCTTCAGACGCACCAGAAGAGG1010.11260633494252618No Hit
TGTCACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT1010.11260633494252618No Hit
TGTCACGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA980.10926159232047095No Hit
TGTCACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG940.10480193549106395No Hit
TGTCACGGGGAATTTGTTGGTTTGAAACATTGAAAAATTTTCATTAGACAATGTTT910.10145719286900873No Hit
TGTCACGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG910.10145719286900873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGCT150.00818207250.34198850
GGACGCG251.9898938E-550.34198850
CGATCGC150.00818207250.34198850
GCGTAGT150.00818207250.34198850
AACCTCA204.0364134E-450.31359549
TTGCGTA150.00820035350.3135948
GTCCGCT150.00820035350.3135949
AAGGCAC150.00820035350.3135948
TTCGAAG150.00820035350.3135948
TGCGTAG150.00820035350.3135949
AAATGGG150.00820035350.3135949
GCATAAG150.00821866550.28523347
CCGTCGA150.00821866550.28523347
TCATCAT252.0032345E-550.28523347
GTTGCGT150.00821866550.28523347
TGCAACC150.00821866550.28523347
ATCCTGT301.0008771E-650.256941
ACTATCC301.0008771E-650.256938
TCTGAGA150.00823700650.256938
GAAGCGA252.0099325E-550.256943
Produced by FastQC (version 0.11.5)