FastQCFastQC Report
Tue 31 May 2016
SRR212881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338615
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18990.5608139036959379No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA16790.4958433619302157No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA16450.4858024600209678No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14690.43382602660839004No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA9900.2923674379457496No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8240.24334421097706835No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7050.20820105429470045No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6920.20436188591763507No Hit
GTATACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC5100.1506135286387195No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA5020.14825096348360234No Hit
GTATACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.13880070286313365No Hit
GTATACGGGGGCTCTCTGCCTTGTTATCAGTCTTAAGCATATAAAAGAAACATTTGTA4480.13230364868656144No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA3860.11399376873440338No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA3670.1083826764910001No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3500.10336222553637613No Hit
GTATACGGGACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAGAAGAAAG3440.10159030167003824No Hit
GTATACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3410.1007043397368693No Hit
GTATACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.1007043397368693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAC150.007172865352.07844546
CAATCGA150.007172865352.07844546
AACACGT150.007172865352.07844547
TCAATCG150.007181267352.06303845
CGTATAC150.00720230352.02454440
GTACGTC150.00720230352.02454438
CATACTA150.007219164651.99379328
TACTAGG150.007219164651.99379330
TTGAACG150.007223384551.9861125
TGATACG150.00722760651.9784321
TACATAC150.00722760651.9784323
GTATAGC203.4512478E-451.9784322
CGGGTAT505.456968E-1251.9554066
GACCGTA150.00724028351.9554029
ACGGGGT33400.051.9554025
TGAATCG150.00724028351.95540215
TACGGGT12550.051.7484134
ACGGGAT43300.051.6554345
GTATACG340650.051.642741
ATACGGG340950.051.59733
Produced by FastQC (version 0.11.5)