FastQCFastQC Report
Tue 31 May 2016
SRR212880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343087
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18730.5459256689994083No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA16760.48850583088254584No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA16430.4788872793198226No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13640.39756679792589056No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA10570.30808512126661747No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7780.22676463987268536No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7450.2171460883099622No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.2084019505256684No Hit
GTATACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC5250.15302241122514115No Hit
GTATACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5210.15185652618723533No Hit
GTATACGGGGGCTCTCTGCCTTGTTATCAGTCTTAAGCATATAAAAGAAACATTTGTA4940.1439868021813709No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA4560.13291089432126546No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA4060.1183373313474425No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4030.1174629175690131No Hit
GTATACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.11513114749320144No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3580.10434671089257243No Hit
GTATACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.10143199829780783No Hit
GTATACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3440.100266113259902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGA203.4133001E-452.09514627
CACGCTA150.007172142652.0799223
CTCTATA251.637784E-552.0799225
AGTAATC150.007172142652.0799225
TAATCGC307.8944686E-752.0799223
TCATGCG203.4206963E-452.0723222
CAACGGT150.00717628852.07231552
TTATGCG150.00717628852.07231521
TACGGCA150.00717628852.07231552
AAACGTA150.00717628852.07231522
GCGAACT251.6420387E-552.05710651
CGTACCT150.007192887452.041950
CCCGATA203.4355267E-452.0267148
TTGAGCG150.007201197652.02670748
CTACTGA203.4380032E-452.0191245
TCTACGT203.4380032E-452.0191238
CGATGCA203.4380032E-452.0191247
TAGACGG150.00720951552.01152444
CGATTGT150.00720951552.01152444
TACGTAC150.00720951552.01152440
Produced by FastQC (version 0.11.5)