FastQCFastQC Report
Tue 31 May 2016
SRR212879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347922
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17600.505860508964653No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15840.45527445806818767No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA15540.4466518357562902No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13920.40008967527204375No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA9380.26960065761866164No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7960.2287869120090135No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT7450.21412845407878778No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.17273986698167978No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA5200.14945878673955657No Hit
GTATACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC5060.14543489632733775No Hit
GTATACGGGGGCTCTCTGCCTTGTTATCAGTCTTAAGCATATAAAAGAAACATTTGTA4290.12330349906013417No Hit
GTATACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4220.12129155385402475No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4010.11525571823569651No Hit
GTATACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.10347146774276994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC650.052.3411186
CGGGTAT401.7826096E-952.3411186
GGCGTGC150.007031704352.3411188
ACCGTAC150.007031704352.34111810
TAATAGG203.4363996E-452.0242226
GTTTCGA150.007202636552.0242225
CTACGTA203.4363996E-452.0242239
CGATCAG203.4363996E-452.0242238
CGGAATG150.007206743652.0167228
TACGGAC150.00723142351.97177536
ACGTACT203.4560112E-451.96429441
TACGTAC203.4560112E-451.96429440
GTTGACG150.007235542451.9642940
TTAGCCG150.007239663551.9568123
CGCACTA203.4633884E-451.94185645
CGACTTA203.4633884E-451.94185645
TACGGGT13650.051.9350664
ACGGGAT44750.051.9317475
TAAGGCG203.4757116E-451.90450322
TCGTTTA203.4781802E-451.89703821
Produced by FastQC (version 0.11.5)