FastQCFastQC Report
Tue 31 May 2016
SRR212878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391474
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC30350.7752749863337028No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21040.5374558719097564No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT16440.41995126113100745No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15690.40079290067795054No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC15400.3933850013027685No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT13250.33846436800400537No Hit
GTATACGGGGTTTTTTAATGGCACATTTTAAAATGCATATTACACTTTATTTGTGT7120.18187670190102026No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.17446880252583824No Hit
GTATACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6410.16374012067212637No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC6310.1611856726117188No Hit
GTATACGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCT5830.14892432192176236No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5670.14483720502511022No Hit
GTATACGGGATGTTTATTTATTTGTTTTCAATTAGAGGAATAGTGTCAAGCACTCC5010.12797784782642016No Hit
GTATACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.1269560686022571No Hit
GTATACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.1256788445720533No Hit
GTATACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT4780.12210261728748269No Hit
GTATACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC4630.11827094519687133No Hit
GTATACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC4240.10830859776128172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCC252.0093208E-550.31307250
CACCTAT204.0769437E-450.25470443
TACCTAG150.00829299250.20293441
ACGTACT150.00829299250.20293441
TACGTAC150.0083098950.1770940
CTACGTA150.00831834850.16417739
TCTACGT204.118607E-450.15127638
AATACGA252.0479818E-550.15127637
ACGCGAA150.00832681350.1512736
TACGCGA150.00833952250.13192435
TGCGTCA150.00835224650.11259533
GCATACG150.00836073750.09971632
TACCGGC150.00836923450.0868431
GTAGACG204.1553396E-450.0611229
CACTAGA150.00839476450.04826428
ATACTAA204.165882E-450.0354227
CTAGCGT150.00841608350.01616326
CACGCGA150.00843316749.9905124
TAGCACG150.00847599149.92649523
CGTAATA150.00847599149.92649523
Produced by FastQC (version 0.11.5)