FastQCFastQC Report
Tue 31 May 2016
SRR212877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350443
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC26260.749337267401546No Hit
GTATACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17040.48624169979140686No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT14000.3994943542887146No Hit
GTATACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13730.3917898203131465No Hit
GTATACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13500.3852266987784033No Hit
GTATACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11440.326443958075921No Hit
GTATACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC7540.21515624509549341No Hit
GTATACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5910.16864368813187877No Hit
GTATACGGGGTTTTTTAATGGCACATTTTAAAATGCATATTACACTTTATTTGTGT5840.16664621636043522No Hit
GTATACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC5500.1569442106134236No Hit
GTATACGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCT4950.14124978955208123No Hit
GTATACGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4690.1338306086867194No Hit
GTATACGGGATGTTTATTTATTTGTTTTCAATTAGAGGAATAGTGTCAAGCACTCC4670.13325990246630695No Hit
GTATACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4560.13012101825403846No Hit
GTATACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT4500.1284088995928011No Hit
GTATACGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4350.12412860293970775No Hit
GTATACGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC4240.12098971872743927No Hit
GTATACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.11556800963352101No Hit
GTATACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC3980.11357053786207744No Hit
GTATACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.1121437723110463No Hit
GTATACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3510.10015894168238487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT150.00822173950.3115248
CGCTAGT150.00822642550.3042747
CGGTCAT204.0652294E-450.28252846
CCCGTTT204.068121E-450.2752845
CGAATAA204.068121E-450.2752845
CAATATA252.0229976E-550.25356343
GCTAGTT150.00827339950.2318741
CGAAGTA150.00827339950.2318741
TACCCGA150.00827810750.2246440
CTACGTA150.00828752950.2101939
GTACGTC301.0169442E-650.20296538
ATCGTTA150.00830639950.18131334
CTAGCCG150.00831584550.16688533
CGTACAG204.1940025E-449.96578627
TGCGTAC204.2118106E-449.92290525
TATAGCG301.0611348E-649.89435624
ACGTATT150.00851126649.87296723
TAAGACG204.2326626E-449.87296723
ATACGTC252.1160722E-549.87296323
GACGTAT150.00851607449.8658422
Produced by FastQC (version 0.11.5)