FastQCFastQC Report
Tue 31 May 2016
SRR212876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57139
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9201.6101086823360578No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7901.3825933250494409No Hit
CTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6461.1305763139011884No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5550.9713155638005566No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3760.6580444179982149No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3680.6440434729344231No Hit
CTCGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.4462801239083638No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA2540.4445300057753898No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA2350.4112777612488843No Hit
CTCGCAGGGGTCCTCTGTGGTTGGTAGAGACTCTCGCATAGACAGCGGTACTGGGTTG2230.3902763436531966No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2190.3832758711213007No Hit
CTCGCAGGGGAGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCT2090.3657746897915609No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2050.358774217259665No Hit
CTCGCAGGGGGTTACTGTTTTCTCTGTCTGCTGTCTAACCCTGTGCCTTGCCTGGGAT1940.33952291779695126No Hit
CTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.33427256339802935No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG1790.31327114580234167No Hit
CTCGCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.26426783807907034No Hit
CTCGCAGGGAAGTTAGTCATTCTCACTTCTCAAAATAACAAATCAGGATGTCAAAACA1460.25551724741420045No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1370.2397661842174347No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1360.2380160660844607No Hit
CTCGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.22926547541959083No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1270.2222650028876949No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1200.2100141759568771No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1130.19776334902605924No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCTTTCTCCT1080.1890127583611894No Hit
CTCGCAGGGTATCCAAAGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACAGA1070.1872626402282154No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCTGA1010.17676193143037155No Hit
CTCGCAGGGGCAACTTGAATTTCAAAAATTTCCATGTAAAAAATGAGCAAATTGAAAT980.17151157703144962No Hit
CTCGCAGGGGACTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCCAGAG980.17151157703144962No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA960.16801134076550167No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA960.16801134076550167No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTCCG950.1662612226325277No Hit
CTCGCAGGGGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCC930.16276098636657974No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAA890.15576051383468384No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA890.15576051383468384No Hit
CTCGCAGGGGAGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCAA830.14525980503684No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCAAA820.143509686903866No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA820.143509686903866No Hit
CTCGCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.143509686903866No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCATCCAAA800.14000945063791806No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAA790.13825933250494407No Hit
CTCGCAGGGATTAACTATGATTATTAATACCTCTGGTTGATTTACTTGTGTTACCTGT780.1365092143719701No Hit
CTCGCAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.12950874184007422No Hit
CTCGCAGGGATTTCAAGTGCCTAGACTGTTACTTTGGCAATGTCCTGGCCCACTTCTC740.12950874184007422No Hit
CTCGCAGGGGTCCAGGGATTAATATTTTCCTCTCTTCAGATTTCCACAAAGTAAGGGA730.12775862370710023No Hit
CTCGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG730.12775862370710023No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCAA730.12775862370710023No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCAGA730.12775862370710023No Hit
CTCGCAGGGGTTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGG730.12775862370710023No Hit
CTCGCAGGGGATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTA730.12775862370710023No Hit
CTCGCAGGGGCAAGGGTATAATTTCATGCAGTAAGCATGTGACGTACATTCCCTTCCT720.12600850557412624No Hit
CTCGCAGGGACTTTTGAAACTGACAAAGGACAGTGTTTAGATTTAGTGTTTTATCTTA710.12425838744115228No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTCTTCCG710.12425838744115228No Hit
CTCGCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.12425838744115228No Hit
CTCGCAGGGGGGTCATTGGGTTTCACTCTGCATATGCAGCCTTCCTGAGGGAATCATG710.12425838744115228No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA690.12075815117520432No Hit
CTCGCAGGGGGGCTTCTGTGCTATTGCTGGGATTGGGAGAAATAAAACATGCAACTTA690.12075815117520432No Hit
CTCGCAGGGGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTG690.12075815117520432No Hit
CTCGCAGGGGTTTTGGCTTTCTTGAATGCTATGATTACAGATATGAGTCACTGGGCTA680.11900803304223034No Hit
CTCGCAGGGGGGTTGGCAAAATGGCTCAGAGGGTAAGAGCACTGACTGCTATTCCGAA680.11900803304223034No Hit
CTCGCAGGGGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG680.11900803304223034No Hit
CTCGCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1155077967762824No Hit
CTCGCAGGGAACTTAGACTAATCTAGAAATGCTATAAGTAGCCAACTCTACAACAGAA660.1155077967762824No Hit
CTCGCAGGGGGAATTAATGAATATTGAATTTAAAACTAATTATGAGTGAAAAATAAAA650.11375767864330842No Hit
CTCGCAGGGGTGTGTTATAATTACATCAAAGGAAAAACGAGCTCAAATGGACCACTTG640.11200756051033445No Hit
CTCGCAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.10850732424438649No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG620.10850732424438649No Hit
CTCGCAGGGACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCTGT620.10850732424438649No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAAAGCACTCACTGCCTGCTCTT620.10850732424438649No Hit
CTCGCAGGGGGTGGTGTAGTTTGCAGGCTGAAGCCATGTCTAAATGAGAGTTCTTGGT610.10675720611141251No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA600.10500708797843855No Hit
CTCGCAGGGCGGAAAAAAAATAATCATGTTGGTGTTTTTGCTTTTCTCTCTCTCCCTC590.10325696984546458No Hit
CTCGCAGGGATTGTGGGCCGAAACTTCGGTAGTTATGTGACACATGAAACCAAACACT590.10325696984546458No Hit
CTCGCAGGGAGCCTGGACCACACAGAGAAACCCTACTTCAAAACACAAAAAAGGAAAA590.10325696984546458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC150.007162261352.0420751
CAACCAA150.007162261352.0420749
GTCGAGC150.007162261352.0420751
CGTATGT150.007162261352.0420749
AACAGTG203.406311E-452.0420752
AGCGCAA401.8280844E-952.0420752
CTGTCGA150.007162261352.0420749
TCGAGCA150.007162261352.0420752
TGTCGAG150.007162261352.0420750
GCGGGGA150.007187146751.996529
CTACAAG150.007187146751.996522
ACAGCAA150.007187146751.996514
GCGGGAC150.007187146751.996545
GGATGCA150.007187146751.99658
CCTATTC203.4210985E-451.996543
ACGCCGG150.007187146751.996527
GGGCAAC203.4210985E-451.99658
TGGTATC800.051.996516
TTTCTTA150.007187146751.996527
AGGATTT150.007187146751.996535
Produced by FastQC (version 0.11.5)