FastQCFastQC Report
Tue 31 May 2016
SRR212875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59753
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9901.6568205780462906No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8061.3488862483892023No Hit
CTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7021.1748364098873696No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5090.8518400749753151No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4010.6710960119157198No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3950.6610546750790756No Hit
CTCGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.48700483657724297No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA2680.44851304537010694No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG2440.40834769802353016No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA2310.38659146821080115No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2300.38491791207136045No Hit
CTCGCAGGGGTCCTCTGTGGTTGGTAGAGACTCTCGCATAGACAGCGGTACTGGGTTG2270.37989724365303834No Hit
CTCGCAGGGGGTTACTGTTTTCTCTGTCTGCTGTCTAACCCTGTGCCTTGCCTGGGAT2190.3665087945375128No Hit
CTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.3280170033303767No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1950.326343447190936No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1710.28617809984435927No Hit
CTCGCAGGGGAGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCT1660.2778103191471558No Hit
CTCGCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.2660954261710709No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1470.24601275249778254No Hit
CTCGCAGGGGCAACTTGAATTTCAAAAATTTCCATGTAAAAAATGAGCAAATTGAAAT1290.21588874198785No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1270.2125416297089686No Hit
CTCGCAGGGTATCCAAAGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACAGA1260.2108680735695279No Hit
CTCGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.20752096129064648No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA1240.20752096129064648No Hit
CTCGCAGGGAAGTTAGTCATTCTCACTTCTCAAAATAACAAATCAGGATGTCAAAACA1210.20250029287232438No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1140.19078539989623952No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1130.18911184375679882No Hit
CTCGCAGGGGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCC1130.18911184375679882No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1060.17739695078071394No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCTGA990.16568205780462905No Hit
CTCGCAGGGGACTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCCAGAG950.15898783324686627No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTCCG870.1455993841313407No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCTTTCTCCT870.1455993841313407No Hit
CTCGCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.1439258279919No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG840.1405787157130186No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCAGA840.1405787157130186No Hit
CTCGCAGGGGAGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCAA830.1389051595735779No Hit
CTCGCAGGGATTAACTATGATTATTAATACCTCTGGTTGATTTACTTGTGTTACCTGT830.1389051595735779No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCAAA820.1372316034341372No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA820.1372316034341372No Hit
CTCGCAGGGGATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTA820.1372316034341372No Hit
CTCGCAGGGGGGTCATTGGGTTTCACTCTGCATATGCAGCCTTCCTGAGGGAATCATG810.1355580472946965No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCAA800.1338844911552558No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA790.1322109350158151No Hit
CTCGCAGGGGTCCAGGGATTAATATTTTCCTCTCTTCAGATTTCCACAAAGTAAGGGA770.1288638227369337No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA740.12384315431861162No Hit
CTCGCAGGGGTGTGTTATAATTACATCAAAGGAAAAACGAGCTCAAATGGACCACTTG730.12216959817917092No Hit
CTCGCAGGGATTTCAAGTGCCTAGACTGTTACTTTGGCAATGTCCTGGCCCACTTCTC730.12216959817917092No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA730.12216959817917092No Hit
CTCGCAGGGGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTG720.12049604203973022No Hit
CTCGCAGGGATTGTGGGCCGAAACTTCGGTAGTTATGTGACACATGAAACCAAACACT720.12049604203973022No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCATCCAAA720.12049604203973022No Hit
CTCGCAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.11882248590028952No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTTCGA700.11714892976084884No Hit
CTCGCAGGGGTTTTGGCTTTCTTGAATGCTATGATTACAGATATGAGTCACTGGGCTA690.11547537362140814No Hit
CTCGCAGGGCGGAAAAAAAATAATCATGTTGGTGTTTTTGCTTTTCTCTCTCTCCCTC690.11547537362140814No Hit
CTCGCAGGGATCAGGAAGCATACAGAACGCAGGCTTCCTAAAATACATAGAAAATAGG690.11547537362140814No Hit
CTCGCAGGGGTTCTCAGCCCAGCCCTCCCTGAGAACCCACATTTGGCTCGTATGTATG690.11547537362140814No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAA680.11380181748196744No Hit
CTCGCAGGGGTTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGG680.11380181748196744No Hit
CTCGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG670.11212826134252674No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTCTTCCG670.11212826134252674No Hit
CTCGCAGGGACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCTGT660.11045470520308603No Hit
CTCGCAGGGGGGTTGGCAAAATGGCTCAGAGGGTAAGAGCACTGACTGCTATTCCGAA650.10878114906364535No Hit
CTCGCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.10878114906364535No Hit
CTCGCAGGGGGAATTAATGAATATTGAATTTAAAACTAATTATGAGTGAAAAATAAAA640.10710759292420464No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAAAGCACTCACTGCCTGCTCTT640.10710759292420464No Hit
CTCGCAGGGGCAAGGGTATAATTTCATGCAGTAAGCATGTGACGTACATTCCCTTCCT630.10543403678476394No Hit
CTCGCAGGGGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG630.10543403678476394No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAA620.10376048064532324No Hit
CTCGCAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.10208692450588254No Hit
CTCGCAGGGATGGAAGGTGGCGGAGGAAGTAGCAACAAATCCACCAGCGGGTTAGCTG610.10208692450588254No Hit
CTCGCAGGGAACTTAGACTAATCTAGAAATGCTATAAGTAGCCAACTCTACAACAGAA600.10041336836644184No Hit
CTCGCAGGGGGGCTTCTGTGCTATTGCTGGGATTGGGAGAAATAAAACATGCAACTTA600.10041336836644184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTAA203.4005885E-452.0637121
TGTCAAA203.4005885E-452.0637149
TCAATTC203.4005885E-452.0637124
TGTTACC203.4005885E-452.0637149
ATGCTAT203.4005885E-452.0637126
TGCTATG203.4005885E-452.0637127
TGGGTTG353.7642167E-852.0637152
TAATACC203.4005885E-452.0637125
TTGAATG203.4005885E-452.0637122
TTGGTAG401.8244464E-952.0637121
TATTAAT203.4005885E-452.0637122
ATACCTC203.4005885E-452.0637127
TAGCGGT150.007152091752.06370527
GCCTCTA150.007152091752.06370551
CGGAGGA150.007152091752.06370521
AATTTCG150.007152091752.06370549
TGCCGAG150.007152091752.06370551
TCGGTGT150.007152091752.06370522
GCGGCAG150.007152091752.06370524
TTCGAAC150.007152091752.06370552
Produced by FastQC (version 0.11.5)