FastQCFastQC Report
Tue 31 May 2016
SRR212874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61678
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8831.4316287817374105No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8131.3181361263335387No Hit
CTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6140.9954927202568177No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA5260.8528162391776647No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG3990.6469081358020689No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3820.6193456337754142No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA3230.5236875385064367No Hit
CTCGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2240.3631764972923895No Hit
CTCGCAGGGGTCCTCTGTGGTTGGTAGAGACTCTCGCATAGACAGCGGTACTGGGTTG2160.3502059081033756No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2080.3372353189143617No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA2070.33561399526573493No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2020.32750737702260124No Hit
CTCGCAGGGGGTTACTGTTTTCTCTGTCTGCTGTCTAACCCTGTGCCTTGCCTGGGAT1920.31129414053633386No Hit
CTCGCAGGGGAGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCT1890.30643016959045366No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTG1730.2804889912124258No Hit
CTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.26913972567203864No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1600.25941178378027824No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1390.22536398715911668No Hit
CTCGCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370.22212133986186322No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCTGA1320.21401472161872953No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1290.20915075067284933No Hit
CTCGCAGGGAAGTTAGTCATTCTCACTTCTCAAAATAACAAATCAGGATGTCAAAACA1260.2042867797269691No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1200.19455883783520866No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1160.1880735432407017No Hit
CTCGCAGGGGCAACTTGAATTTCAAAAATTTCCATGTAAAAAATGAGCAAATTGAAAT1140.18483089594344823No Hit
CTCGCAGGGTATCCAAAGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACAGA1140.18483089594344823No Hit
CTCGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.16861765945718085No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1010.16375368851130062No Hit
CTCGCAGGGGACTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCCAGAG990.16051104121404716No Hit
CTCGCAGGGGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCC900.1459191283764065No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCAGA890.14429780472777975No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA880.142676481079153No Hit
CTCGCAGGGATTAACTATGATTATTAATACCTCTGGTTGATTTACTTGTGTTACCTGT860.13943383378189955No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA860.13943383378189955No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCAAA850.1378125101332728No Hit
CTCGCAGGGGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTG850.1378125101332728No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG820.1329485391873926No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCTTTCTCCT820.1329485391873926No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCAA800.12970589189013912No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTCCG800.12970589189013912No Hit
CTCGCAGGGGTTTTGGCTTTCTTGAATGCTATGATTACAGATATGAGTCACTGGGCTA780.12646324459288563No Hit
CTCGCAGGGGGAATTAATGAATATTGAATTTAAAACTAATTATGAGTGAAAAATAAAA780.12646324459288563No Hit
CTCGCAGGGGTTCTCAGCCCAGCCCTCCCTGAGAACCCACATTTGGCTCGTATGTATG760.12322059729563214No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCTGA740.11997794999837869No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAA730.11835662634975194No Hit
CTCGCAGGGGAGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCCAA720.1167353027011252No Hit
CTCGCAGGGACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCTGT720.1167353027011252No Hit
CTCGCAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.11511397905249847No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAA710.11511397905249847No Hit
CTCGCAGGGGGGTTGGCAAAATGGCTCAGAGGGTAAGAGCACTGACTGCTATTCCGAA710.11511397905249847No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCATCCAAA710.11511397905249847No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTTCGA700.11349265540387171No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTATTCCAAA680.11025000810661824No Hit
CTCGCAGGGGTCCAGGGATTAATATTTTCCTCTCTTCAGATTTCCACAAAGTAAGGGA670.1086286844579915No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTCTTCCG670.1086286844579915No Hit
CTCGCAGGGGATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTA670.1086286844579915No Hit
CTCGCAGGGGTATGTTGTAGGGAAATGGTGTGGGTGAGACCAGAGCTTCCCCTGGTCA650.10538603716073802No Hit
CTCGCAGGGGTGTGTTATAATTACATCAAAGGAAAAACGAGCTCAAATGGACCACTTG640.10376471351211129No Hit
CTCGCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10376471351211129No Hit
CTCGCAGGGGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG640.10376471351211129No Hit
CTCGCAGGGGGGTCATTGGGTTTCACTCTGCATATGCAGCCTTCCTGAGGGAATCATG640.10376471351211129No Hit
CTCGCAGGGACTTTTGAAACTGACAAAGGACAGTGTTTAGATTTAGTGTTTTATCTTA630.10214338986348455No Hit
CTCGCAGGGATGGAAGGTGGCGGAGGAAGTAGCAACAAATCCACCAGCGGGTTAGCTG630.10214338986348455No Hit
CTCGCAGGGATTGTGGGCCGAAACTTCGGTAGTTATGTGACACATGAAACCAAACACT630.10214338986348455No Hit
CTCGCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.10214338986348455No Hit
CTCGCAGGGGGGCTTCTGTGCTATTGCTGGGATTGGGAGAAATAAAACATGCAACTTA620.10052206621485782No Hit
CTCGCAGGGGTTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGG620.10052206621485782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGAAG150.007008879452.33224510
GGTCCTC307.5318167E-752.3322459
GGATAGT150.007008879452.3322458
GTATGTT150.007008879452.33224510
AAGTTAG307.5318167E-752.33224510
GAAGTTA307.5318167E-752.3322459
GGGCCTC150.007008879452.3322458
GGGCAAC150.007008879452.3322458
GGACCAA150.007008879452.3322458
GGATTGC150.007008879452.3322458
GATTCCT150.007008879452.3322459
GACCTTA150.007008879452.3322459
AGGGATC2850.052.3322456
ACTGGTA150.007008879452.33224510
GGATCGA203.3159542E-452.332248
GGATCAT353.6165147E-852.332248
GGACCTT251.5761216E-552.332248
AGGGATA353.6165147E-852.332246
TACTTAG150.007123105752.1189938
CAAATCA150.007123105752.1189939
Produced by FastQC (version 0.11.5)