FastQCFastQC Report
Tue 31 May 2016
SRR212873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86215
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC16241.8836629356840457No Hit
CTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9931.151771733457055No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8460.9812677608304818No Hit
CTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7020.8142434611146552No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT6010.6970944731195268No Hit
CTCGCAGGGGCAAGGGTATAATTTCATGCAGTAAGCATGTGACGTACATTCCCTTC3080.35724641883662933No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3030.3514469639853854No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC3030.3514469639853854No Hit
CTCGCAGGGGCAACTTGAATTTCAAAAATTTCCATGTAAAAAATGAGCAAATTGAA3020.3502870730151366No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2990.34680740010439015No Hit
CTCGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.30505132517543354No Hit
CTCGCAGGGCGGCTTGGCATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTT2600.30157165226468713No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2510.29113263353244795No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2400.2783738328597112No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC2400.2783738328597112No Hit
CTCGCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.2551760134547353No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2150.24937655860349126No Hit
CTCGCAGGGGAGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGAC2110.24473699472249608No Hit
CTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.22269906628776898No Hit
CTCGCAGGGGGTTACTGTTTTCTCTGTCTGCTGTCTAACCCTGTGCCTTGCCTGGG1900.22037928434727136No Hit
CTCGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.18210288232906108No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1570.18210288232906108No Hit
CTCGCAGGGGGTGGTGTAGTTTGCAGGCTGAAGCCATGTCTAAATGAGAGTTCTTG1520.17630342747781708No Hit
CTCGCAGGGGACTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCCAG1490.17282375456707072No Hit
CTCGCAGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCG1440.16702429971582672No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCT1430.1658644087455779No Hit
CTCGCAGGGAAGTTAGTCATTCTCACTTCTCAAAATAACAAATCAGGATGTCAAAA1330.15426549904308995No Hit
CTCGCAGGGTATCCAAAGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACA1330.15426549904308995No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG1330.15426549904308995No Hit
CTCGCAGGGGTTCTCAGCCCAGCCCTCCCTGAGAACCCACATTTGGCTCGTATGTA1300.15078582613234356No Hit
CTCGCAGGGGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTT1250.1449863712810996No Hit
CTCGCAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1220.14150669837035318No Hit
CTCGCAGGGGTGGGGTCCCTTGGCACGGTACTGTCTGCAGCCCCTTGTCTCTCACC1210.14034680740010438No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1180.136867134489358No Hit
CTCGCAGGGGAGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCC1170.1357072435191092No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTTC1170.1357072435191092No Hit
CTCGCAGGGAGCCTGGACCACACAGAGAAACCCTACTTCAAAACACAAAAAAGGAA1170.1357072435191092No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1150.13338746157861162No Hit
CTCGCAGGGGTCCTCTGTGGTTGGTAGAGACTCTCGCATAGACAGCGGTACTGGGT1140.13222757060836282No Hit
CTCGCAGGGGGAATTAATGAATATTGAATTTAAAACTAATTATGAGTGAAAAATAA1110.1287478976976164No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1110.1287478976976164No Hit
CTCGCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.12758800672736764No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCA1070.12410833381662123No Hit
CTCGCAGGGATTGGTGGCGTGCAGCAGGACACGATCCTGGCCGAAGGCCTTCACTT1060.12294844284637245No Hit
CTCGCAGGGGGGTAACAAGGGAATGAGATGTGGGTACTTGTGGGTTTTTGGGGGAG1040.12062866090587485No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCTTTCTC1040.12062866090587485No Hit
CTCGCAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1040.12062866090587485No Hit
CTCGCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1030.11946876993562605No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCA1030.11946876993562605No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCC1020.11830887896537726No Hit
CTCGCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1020.11830887896537726No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCT1010.11714898799512845No Hit
CTCGCAGGGATTAACTATGATTATTAATACCTCTGGTTGATTTACTTGTGTTACCT1000.11598909702487967No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC1000.11598909702487967No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC990.11482920605463086No Hit
CTCGCAGGGAGCTTCCTTGAAGGAAAAAGATGAGAGAGAGAGAGAGAGAGAGAGAG990.11482920605463086No Hit
CTCGCAGGGGGGCTTCTGTGCTATTGCTGGGATTGGGAGAAATAAAACATGCAACT980.11366931508438208No Hit
CTCGCAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC970.11250942411413327No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTC960.11134953314388449No Hit
CTCGCAGGGGGTGGGATCAGCAGGAAGACTGATGTTGGAGACAATCGGGGGCGGAT940.1090297512033869No Hit
CTCGCAGGGCGGAAAAAAAATAATCATGTTGGTGTTTTTGCTTTTCTCTCTCTCCC920.10670996926288928No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTCTTC920.10670996926288928No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCATCCA900.10439018732239169No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC890.1032302963521429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAT150.0082589650.22150850
CACGTAT150.0082589650.22150850
AGGCTCC150.0082589650.22150850
ATCTGAC150.0082589650.22150850
CTTGTTG150.00827807250.19217348
CGTATGT150.00827807250.19217349
TCCTCAA150.00827807250.19217348
CATTCCC550.050.19217347
GTAATGG150.00827807250.19217349
TAGTCAG150.00827807250.19217346
TCTCTCA204.084027E-450.1921748
GACAAGC204.084027E-450.1921746
TGCCCAG150.00829721550.16287243
GTATTGC150.00829721550.16287239
CAAAACA252.031719E-550.16287239
CGACCAT150.00829721550.16287239
TGACGTA550.050.16287240
CACTCAC150.00829721550.16287241
CTAAATG150.00829721550.16287240
TAACAGG150.00829721550.16287241
Produced by FastQC (version 0.11.5)