SRR212872_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212872_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78892
Sequences flagged as poor quality	0
Sequence length	56
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1429	1.8113370176950767	No Hit
CTCGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	943	1.1953049738883537	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	691	0.8758809511737565	No Hit
CTCGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	635	0.804897835014957	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	511	0.6477209349490443	No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	284	0.3599858033767682	No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC	274	0.34731024691983975	No Hit
CTCGCAGGGGCAAGGGTATAATTTCATGCAGTAAGCATGTGACGTACATTCCCTTC	263	0.33336713481721847	No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	258	0.32702935658875426	No Hit
CTCGCAGGGGCAACTTGAATTTCAAAAATTTCCATGTAAAAAATGAGCAAATTGAA	249	0.31562135577751865	No Hit
CTCGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	246	0.3118186888404401	No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	244	0.3092835775490544	No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	241	0.30548091061197585	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC	220	0.2788622420524261	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	208	0.26365157430411196	No Hit
CTCGCAGGGCGGCTTGGCATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTT	207	0.2623840186584191	No Hit
CTCGCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	194	0.24590579526441209	No Hit
CTCGCAGGGGGTTACTGTTTTCTCTGTCTGCTGTCTAACCCTGTGCCTTGCCTGGG	193	0.24463823961871925	No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	170	0.21548445976778383	No Hit
CTCGCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	157	0.19900623637377682	No Hit
CTCGCAGGGGAGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGAC	153	0.19393601379100542	No Hit
CTCGCAGGGGGTGGTGTAGTTTGCAGGCTGAAGCCATGTCTAAATGAGAGTTCTTG	145	0.18379556862546265	No Hit
CTCGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	139	0.17619023475130557	No Hit
CTCGCAGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCG	138	0.17492267910561274	No Hit
CTCGCAGGGGACTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTCTTCCAG	137	0.17365512345991987	No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG	134	0.16985245652284134	No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	123	0.15590934442022006	No Hit
CTCGCAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	121	0.15337423312883436	No Hit
CTCGCAGGGGTCCTCTGTGGTTGGTAGAGACTCTCGCATAGACAGCGGTACTGGGT	119	0.15083912183744866	No Hit
CTCGCAGGGGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTT	116	0.14703645490037012	No Hit
CTCGCAGGGGAGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTCC	116	0.14703645490037012	No Hit
CTCGCAGGGGTGGGGTCCCTTGGCACGGTACTGTCTGCAGCCCCTTGTCTCTCACC	115	0.1457688992546773	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	114	0.14450134360898442	No Hit
CTCGCAGGGGGGTAACAAGGGAATGAGATGTGGGTACTTGTGGGTTTTTGGGGGAG	113	0.1432337879632916	No Hit
CTCGCAGGGGAGGCTTAGGTGCAGGATCGGTTTAAGAGACTTTGAAAGCAACTTGG	112	0.14196623231759875	No Hit
CTCGCAGGGTATCCAAAGACTGTACTGGCCAGTGTCCTTTACTTTCCCTCCTGACA	110	0.13943112102621305	No Hit
CTCGCAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	108	0.13689600973482735	No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCTTTCTC	106	0.13436089844344165	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCT	105	0.13309334279774881	No Hit
CTCGCAGGGGGGCTAGAGAGATGGCTCAGTGGATAAGAGTACTGACTACTGTTTCA	104	0.13182578715205598	No Hit
CTCGCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	104	0.13182578715205598	No Hit
CTCGCAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	103	0.13055823150636311	No Hit
CTCGCAGGGAGCCTGGACCACACAGAGAAACCCTACTTCAAAACACAAAAAAGGAA	102	0.12929067586067028	No Hit
CTCGCAGGGAGCTTCCTTGAAGGAAAAAGATGAGAGAGAGAGAGAGAGAGAGAGAG	100	0.12675556456928458	No Hit
CTCGCAGGGGTTCTCAGCCCAGCCCTCCCTGAGAACCCACATTTGGCTCGTATGTA	100	0.12675556456928458	No Hit
CTCGCAGGGATTGGTGGCGTGCAGCAGGACACGATCCTGGCCGAAGGCCTTCACTT	99	0.12548800892359174	No Hit
CTCGCAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	98	0.12422045327789889	No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCATCAGTTAAGAGCACTGACTGCTCCTCT	98	0.12422045327789889	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC	97	0.12295289763220604	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCATCCA	97	0.12295289763220604	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC	97	0.12295289763220604	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTT	96	0.12168534198651319	No Hit
CTCGCAGGGAAGTTAGTCATTCTCACTTCTCAAAATAACAAATCAGGATGTCAAAA	95	0.12041778634082037	No Hit
CTCGCAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	95	0.12041778634082037	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCC	93	0.11788267504943468	No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAAAGCACTCACTGCCTGCTC	92	0.11661511940374183	No Hit
CTCGCAGGGGGAATTAATGAATATTGAATTTAAAACTAATTATGAGTGAAAAATAA	91	0.11534756375804898	No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC	91	0.11534756375804898	No Hit
CTCGCAGGGCGGAAAAAAAATAATCATGTTGGTGTTTTTGCTTTTCTCTCTCTCCC	90	0.11408000811235613	No Hit
CTCGCAGGGATTAACTATGATTATTAATACCTCTGGTTGATTTACTTGTGTTACCT	89	0.1128124524666633	No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTC	89	0.1128124524666633	No Hit
CTCGCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86	0.10900978552958475	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC	86	0.10900978552958475	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTCTTTC	86	0.10900978552958475	No Hit
CTCGCAGGGGGGCTTCTGTGCTATTGCTGGGATTGGGAGAAATAAAACATGCAACT	83	0.10520711859250621	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGTCTGCTCTTCCA	83	0.10520711859250621	No Hit
CTCGCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.10393956294681336	No Hit
CTCGCAGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.10393956294681336	No Hit
CTCGCAGGGACGTGGGCTGCGTTTCTGTAATTGTTCTCCATGTACCCTTTGCCCTT	81	0.10267200730112053	No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGTTCTTC	80	0.10140445165542768	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGT	15	0.008207376	50.297054	49
AGATAGT	15	0.008207376	50.297054	49
ACGCACC	15	0.008207376	50.297054	50
TGCGGAT	15	0.008207376	50.297054	50
ATCCCAA	15	0.008207376	50.297054	49
AACGCAC	15	0.008207376	50.297054	49
TCATCCA	15	0.008207376	50.297054	50
GATAGTT	15	0.008207376	50.297054	50
TAAACGC	20	4.0527774E-4	50.264874	47
CGTTCTT	15	0.008228184	50.264874	47
TAGCAAG	40	2.5083864E-9	50.264874	47
CTCGCCC	15	0.008228184	50.264874	48
TCATCTC	15	0.008228184	50.264874	47
CTACAGA	20	4.0527774E-4	50.264874	48
CTACACC	15	0.008228184	50.264874	47
GACAGCG	35	5.017864E-8	50.23274	41
AATCAGG	40	2.5211193E-9	50.23274	41
CCCTACA	20	4.065603E-4	50.23274	46
TTAGACC	20	4.065603E-4	50.23274	41
ACAATCG	25	2.0134343E-5	50.23274	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content