SRR212871_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212871_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29074
Sequences flagged as poor quality	0
Sequence length	58
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	362	1.2450987136272957	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	226	0.7773268212148311	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	161	0.5537593726353442	No Hit
TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGATTG	140	0.48152988924812545	No Hit
TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGGCT	136	0.46777189241246475	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	132	0.45401389557680405	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	95	0.3267524248469423	No Hit
TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91	0.3129944280112816	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86	0.29579693196670565	No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.28891793354887524	No Hit
TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCTGC	80	0.27515993671321454	No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	80	0.27515993671321454	No Hit
TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATG	76	0.26140193987755383	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	69	0.23732544541514755	No Hit
TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTTAC	62	0.2132489509527413	No Hit
TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59	0.20293045332599574	No Hit
TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	59	0.20293045332599574	No Hit
TTCCGCGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA	58	0.19949095411708057	No Hit
TTCCGCGGGACTTCATTTTCTTTGGAAGAAAATGATGTCTAAAGAACATATAGAGGCA	56	0.1926119556992502	No Hit
TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTT	53	0.18229345807250463	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	49	0.16853546123684393	No Hit
TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA	49	0.16853546123684393	No Hit
TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTGCA	49	0.16853546123684393	No Hit
TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTAT	48	0.16509596202792873	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAA	45	0.1547774644011832	No Hit
TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCTGT	44	0.15133796519226803	No Hit
TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43	0.14789846598335282	No Hit
TTCCGCGGGGTGGGTCATCAGCCCATGCCTCACCCTGGCAGTGTGCTCTAACTGAGGC	42	0.14445896677443762	No Hit
TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	40	0.13757996835660727	No Hit
TTCCGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT	39	0.1341404691476921	No Hit
TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.1341404691476921	No Hit
TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCACCA	39	0.1341404691476921	No Hit
TTCCGCGGGGATGAAAGGAAAAAGAATGCCAAAATCAGCAGCCTCCTGGAGGAGCAGT	38	0.13070096993877692	No Hit
TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	38	0.13070096993877692	No Hit
TTCCGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	37	0.12726147072986171	No Hit
TTCCGCGGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTTTTCAG	37	0.12726147072986171	No Hit
TTCCGCGGGGGTGCTATGTGGGGACATTGAGACAAACCAATCTTTTGTACTTCCACTT	36	0.12382197152094655	No Hit
TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTTTC	36	0.12382197152094655	No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAG	35	0.12038247231203136	No Hit
TTCCGCGGGGTTGTATGATGCCACTTTCCATGTGTATGTGATACCACTGTACATGTGT	34	0.11694297310311619	No Hit
TTCCGCGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT	32	0.11006397468528582	No Hit
TTCCGCGGGAGTGACAAATTATATGTTATAGAAGGTCCAATGTGGGGATAGTGTGTGG	32	0.11006397468528582	No Hit
TTCCGCGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGA	31	0.10662447547637065	No Hit
TTCCGCGGGGGGTCCTAACAGTCTTCACTGCCATCCCCAAGGCCATCACCTCTGGTTT	31	0.10662447547637065	No Hit
TTCCGCGGGATTTTTGAAATAAATTTTTTAATTTATAGAGTATTTCTGAGGGGTGGGG	30	0.10318497626745546	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCTCTTCCGA	30	0.10318497626745546	No Hit
TTCCGCGGGAATTTGGGTTCCTTAGCCATTCCCAGAGAGGGGGAGGTGAGGCCCAGAA	30	0.10318497626745546	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTTAC	25	1.5974561E-5	52.09672	47
GTGTTGG	15	0.007097281	52.096718	50
TTACCTT	15	0.007097281	52.096718	39
CCTAATA	20	3.3607965E-4	52.096718	39
TGTTACT	15	0.007097281	52.096718	48
ATTACCT	15	0.007097281	52.096718	38
CACCTGA	15	0.007097281	52.096718	40
CTAATAT	20	3.3607965E-4	52.096718	40
ACCTTTA	15	0.007097281	52.096718	41
AGACTCT	15	0.007097281	52.096718	39
ACCTAAT	20	3.3607965E-4	52.096718	38
TGGTGTT	15	0.007097281	52.096718	48
GTTACTT	15	0.007097281	52.096718	49
GACTTCT	20	3.3607965E-4	52.096718	44
ACAGTGG	20	3.389647E-4	52.0069	27
TCGAACT	20	3.389647E-4	52.0069	21
TTCGAAC	20	3.389647E-4	52.0069	20
TGTTAAG	20	3.389647E-4	52.0069	31
GCTGTTA	20	3.389647E-4	52.0069	29
CGAACTT	20	3.389647E-4	52.0069	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content