##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212871_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29074
Sequences flagged as poor quality	0
Sequence length	58
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.567998899360255	39.0	38.0	39.0	34.0	40.0
2	37.2425534842127	39.0	38.0	39.0	33.0	40.0
3	37.048565728829885	39.0	36.0	39.0	33.0	40.0
4	37.33249638852583	39.0	38.0	39.0	33.0	40.0
5	36.22779803260645	38.0	35.0	39.0	31.0	40.0
6	36.460445759097475	38.0	35.0	39.0	31.0	40.0
7	36.92556923711908	39.0	36.0	39.0	33.0	40.0
8	37.185836142257685	39.0	38.0	39.0	33.0	40.0
9	37.18662722707574	39.0	38.0	39.0	33.0	40.0
10	35.28561601430832	38.0	33.0	39.0	28.0	40.0
11	35.5	38.0	35.0	39.0	28.0	40.0
12	35.91621379927083	38.0	35.0	39.0	30.0	40.0
13	35.960789709018364	38.0	35.0	39.0	30.0	40.0
14	35.987549012863724	38.0	35.0	39.0	30.0	40.0
15	35.98665474306941	38.0	35.0	39.0	30.0	40.0
16	35.666024626814334	38.0	35.0	39.0	29.0	40.0
17	35.37617802847905	38.0	34.0	39.0	28.0	40.0
18	35.33555754282177	38.0	34.0	39.0	28.0	40.0
19	35.23897640503543	38.0	33.0	39.0	28.0	40.0
20	35.09486138818188	38.0	33.0	39.0	28.0	40.0
21	35.01423952672491	38.0	33.0	39.0	27.0	40.0
22	34.894304189310034	38.0	33.0	39.0	27.0	40.0
23	34.74833184288368	38.0	33.0	39.0	27.0	40.0
24	34.65931760335695	38.0	33.0	39.0	27.0	40.0
25	34.56947788402009	38.0	33.0	39.0	27.0	40.0
26	34.20279287335764	38.0	33.0	39.0	26.0	40.0
27	34.088979844534634	37.0	33.0	39.0	25.0	40.0
28	33.80453325995735	37.0	33.0	39.0	25.0	40.0
29	33.86087225699938	37.0	33.0	39.0	25.0	40.0
30	33.508461168053934	36.0	32.0	39.0	23.0	40.0
31	33.121379927082614	36.0	32.0	38.0	22.0	40.0
32	32.8890073605283	36.0	31.0	38.0	22.0	40.0
33	32.747884707986515	36.0	31.0	38.0	22.0	40.0
34	32.33738047740249	36.0	30.0	38.0	19.0	39.0
35	32.1819839031437	35.0	30.0	38.0	19.0	39.0
36	33.10222191648896	37.0	32.0	39.0	22.0	40.0
37	33.027550388663414	36.0	32.0	39.0	22.0	40.0
38	32.850622549356814	36.0	32.0	39.0	21.0	40.0
39	32.86489647107381	36.0	32.0	39.0	21.0	40.0
40	32.61677099814267	36.0	31.0	39.0	20.0	40.0
41	32.86830157529064	37.0	32.0	39.0	19.0	40.0
42	32.70427185801747	36.0	32.0	39.0	19.0	40.0
43	32.59568686799202	36.0	32.0	39.0	18.0	40.0
44	32.4159730343262	36.0	31.0	39.0	18.0	40.0
45	32.22563114810484	36.0	31.0	39.0	17.0	40.0
46	31.88642773612162	36.0	31.0	39.0	12.0	40.0
47	31.69759922955218	36.0	30.0	39.0	12.0	40.0
48	31.523698149549425	36.0	30.0	39.0	10.0	40.0
49	31.476405035426843	36.0	30.0	39.0	9.0	40.0
50	31.12602325101465	35.0	30.0	39.0	2.0	40.0
51	30.816571507188552	36.0	30.0	39.0	2.0	40.0
52	30.736465570612918	35.0	29.0	38.0	2.0	40.0
53	30.53687143151957	35.0	29.0	38.0	2.0	39.0
54	30.304808419894062	35.0	29.0	38.0	2.0	39.0
55	30.093382403522046	35.0	29.0	38.0	2.0	39.0
56	29.996594895783176	35.0	29.0	39.0	2.0	39.0
57	29.646591456283964	35.0	29.0	38.0	2.0	39.0
58	29.373151269175207	35.0	28.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-22.806436916024467
1	2	-18.227732069932642
1	3	-14.206439183145893
1	4	-18.308399051935602
1	5	-16.345697786357107
1	6	-13.447039059415957
1	7	-11.32254547458653
1	8	-14.249342377457467
1	9	-14.374540343014736
1	10	-11.521042291172368
1	11	0.8116166162622278
1	12	-0.20692604467146936
1	13	0.1976188849841023
1	14	2.7489118972835698
1	15	-0.7305209331096343
1	16	0.6576473718272915
1	17	3.825058295281309
1	18	1.0614722448695844
1	19	0.9377753281553218
1	20	0.991194425767489
1	21	4.090310862711782
1	22	1.7418114449129298
1	23	1.7339306955029556
1	24	1.6632929105202585
1	25	1.8912141904381983
1	26	5.897914439757024
1	27	3.3859963212161404
1	28	5.94724514569652
1	29	6.047141327142736
1	30	-10.152355926455932
1	31	-10.15363056041113
1	32	-9.841139778952932
1	33	-9.447883788621148
1	34	-9.495100544877602
1	35	5.422931375446538
1	36	-5.3001035084136525
1	37	-2.9145994967975675
1	38	-2.676374826726505
1	39	-2.632810010646349
1	40	-2.1243504251965675
1	41	-7.782021235777783
1	42	-3.6242081281064777
1	43	-3.4304205949806175
1	44	-3.2542653482708097
1	45	-3.1817872512705314
1	46	-1.974481187929829
1	47	-1.2422454430668886
1	48	-2.5728564401078273
1	49	-1.6310995434331872
1	50	-5.0418843758131295
1	51	1.959086417233351
1	52	2.383129838968099
1	53	2.8548713255514215
1	54	1.1303302773938277
1	55	1.2357288292266695
1	56	7.450317534385501
1	57	7.935962959895779
1	58	8.19600598012094
3	1	0.19356308397553335
3	2	1.7722679300673576
3	3	-1.2064391831458927
3	4	1.6916009480643979
3	5	-2.345697786357107
3	6	1.5529609405840432
3	7	1.67745452541347
3	8	3.7506576225425334
3	9	3.6254596569852637
3	10	3.4789577088276324
3	11	3.811616616262228
3	12	2.7930739553285306
3	13	5.197618884984102
3	14	5.74891189728357
3	15	5.269479066890366
3	16	-7.3423526281727085
3	17	2.825058295281309
3	18	3.0614722448695844
3	19	2.937775328155322
3	20	6.991194425767489
3	21	-5.909689137288218
3	22	-7.25818855508707
3	23	4.733930695502956
3	24	0.6632929105202585
3	25	-0.10878580956180173
3	26	1.8979144397570238
3	27	-0.6140036787838596
3	28	7.94724514569652
3	29	5.047141327142736
3	30	4.847644073544068
3	31	5.8463694395888695
3	32	-3.8411397789529325
3	33	8.552116211378852
3	34	6.504899455122398
3	35	-7.577068624553462
3	36	-3.3001035084136525
3	37	-3.9145994967975675
3	38	0.32362517327349494
3	39	3.367189989353651
3	40	-24.124350425196567
3	41	-24.782021235777783
3	42	-24.624208128106478
3	43	-24.430420594980617
3	44	-24.25426534827081
3	45	-24.18178725127053
3	46	-23.97448118792983
3	47	-23.24224544306689
3	48	-23.572856440107827
3	49	-23.631099543433187
3	50	-23.04188437581313
3	51	-23.04091358276665
3	52	-22.6168701610319
3	53	-22.14512867444858
3	54	-21.869669722606172
3	55	-21.76427117077333
3	56	-21.5496824656145
3	57	-21.06403704010422
3	58	-20.80399401987906
4	1	-31.806436916024467
4	2	-30.227732069932642
4	3	-29.206439183145893
4	4	-30.308399051935602
4	5	-28.345697786357107
4	6	-29.447039059415957
4	7	-29.32254547458653
4	8	-30.249342377457467
4	9	-30.374540343014736
4	10	-27.521042291172368
4	11	-28.188383383737772
4	12	-29.20692604467147
4	13	-28.802381115015898
4	14	-29.25108810271643
4	15	-29.730520933109634
4	16	-28.34235262817271
4	17	-28.17494170471869
4	18	-27.938527755130416
4	19	-28.062224671844678
4	20	-28.00880557423251
4	21	-27.90968913728822
4	22	-27.25818855508707
4	23	-27.266069304497044
4	24	-27.33670708947974
4	25	-27.1087858095618
4	26	-26.102085560242976
4	27	-25.61400367878386
4	28	-26.05275485430348
4	29	-25.952858672857264
4	30	-25.152355926455932
4	31	-25.15363056041113
4	32	-24.841139778952932
4	33	-24.447883788621148
4	34	-24.4951005448776
4	35	-23.577068624553462
4	36	-25.300103508413653
4	37	-24.914599496797567
4	38	-24.676374826726505
4	39	-24.63281001064635
4	40	-24.124350425196567
4	41	-24.782021235777783
4	42	-24.624208128106478
4	43	-24.430420594980617
4	44	-24.25426534827081
4	45	-24.18178725127053
4	46	-23.97448118792983
4	47	-23.24224544306689
4	48	-23.572856440107827
4	49	-23.631099543433187
4	50	-23.04188437581313
4	51	-23.04091358276665
4	52	-22.6168701610319
4	53	-22.14512867444858
4	54	-21.869669722606172
4	55	-21.76427117077333
4	56	-21.5496824656145
4	57	-21.06403704010422
4	58	-20.80399401987906
5	1	4.193563083975533
5	2	5.772267930067358
5	3	6.793560816854107
5	4	5.691600948064398
5	5	7.654302213642893
5	6	4.552960940584043
5	7	2.67745452541347
5	8	1.7506576225425334
5	9	1.6254596569852637
5	10	2.4789577088276324
5	11	-3.188383383737772
5	12	3.7930739553285306
5	13	4.197618884984102
5	14	4.74891189728357
5	15	6.269479066890366
5	16	-2.3423526281727085
5	17	-0.17494170471869097
5	18	-1.9385277551304156
5	19	1.9377753281553218
5	20	-2.008805574232511
5	21	4.090310862711782
5	22	2.74181144491293
5	23	4.733930695502956
5	24	2.6632929105202585
5	25	4.891214190438198
5	26	7.897914439757024
5	27	2.3859963212161404
5	28	5.94724514569652
5	29	1.047141327142736
5	30	3.8476440735440676
5	31	2.8463694395888695
5	32	3.1588602210470675
5	33	1.552116211378852
5	34	4.504899455122398
5	35	-0.577068624553462
5	36	6.6998964915863475
5	37	2.0854005032024325
5	38	7.323625173273495
5	39	4.367189989353651
5	40	5.8756495748034325
5	41	10.217978764222217
5	42	10.375791871893522
5	43	11.569579405019383
5	44	11.74573465172919
5	45	11.818212748729469
5	46	9.025518812070171
5	47	3.7577545569331114
5	48	9.427143559892173
5	49	9.368900456566813
5	50	-0.04188437581312954
5	51	8.959086417233351
5	52	9.383129838968099
5	53	5.8548713255514215
5	54	1.1303302773938277
5	55	7.2357288292266695
5	56	10.450317534385501
5	57	12.935962959895779
5	58	13.19600598012094
7	1	-11.806436916024467
7	2	-21.227732069932642
7	3	-22.206439183145893
7	4	-23.308399051935602
7	5	-23.345697786357107
7	6	-17.447039059415957
7	7	-17.32254547458653
7	8	-18.249342377457467
7	9	-18.374540343014736
7	10	-18.521042291172368
7	11	-13.188383383737772
7	12	-14.20692604467147
7	13	0.1976188849841023
7	14	-0.25108810271643023
7	15	-0.7305209331096343
7	16	0.6576473718272915
7	17	-13.174941704718691
7	18	-12.938527755130416
7	19	0.9377753281553218
7	20	0.991194425767489
7	21	1.0903108627117817
7	22	1.7418114449129298
7	23	1.7339306955029556
7	24	1.6632929105202585
7	25	1.8912141904381983
7	26	-3.102085560242976
7	27	-2.6140036787838596
7	28	-3.0527548543034797
7	29	-2.952858672857264
7	30	-2.1523559264559324
7	31	-4.1536305604111305
7	32	-3.8411397789529325
7	33	-6.447883788621148
7	34	-3.4951005448776016
7	35	-2.577068624553462
7	36	-9.300103508413653
7	37	-8.914599496797567
7	38	-8.676374826726505
7	39	-10.632810010646349
7	40	-8.124350425196567
7	41	-6.782021235777783
7	42	-4.624208128106478
7	43	-6.4304205949806175
7	44	-4.25426534827081
7	45	-11.181787251270531
7	46	-3.974481187929829
7	47	-5.2422454430668886
7	48	-3.5728564401078273
7	49	-2.631099543433187
7	50	-5.0418843758131295
7	51	5.959086417233351
7	52	6.383129838968099
7	53	6.8548713255514215
7	54	7.130330277393828
7	55	7.2357288292266695
7	56	-1.5496824656144987
7	57	-7.064037040104221
7	58	-0.8039940198790596
8	1	2.1935630839755333
8	2	3.7722679300673576
8	3	1.7935608168541073
8	4	3.691600948064398
8	5	2.654302213642893
8	6	2.552960940584043
8	7	-1.32254547458653
8	8	1.7506576225425334
8	9	1.6254596569852637
8	10	0.47895770882763244
8	11	4.811616616262228
8	12	6.793073955328531
8	13	-7.802381115015898
8	14	6.74891189728357
8	15	0.2694790668903657
8	16	-5.3423526281727085
8	17	-5.174941704718691
8	18	-4.938527755130416
8	19	-5.062224671844678
8	20	-3.008805574232511
8	21	6.090310862711782
8	22	-2.25818855508707
8	23	0.7339306955029556
8	24	-2.3367070894797415
8	25	-2.1087858095618017
8	26	-13.102085560242976
8	27	-9.61400367878386
8	28	-13.05275485430348
8	29	-12.952858672857264
8	30	-15.152355926455932
8	31	-8.15363056041113
8	32	3.1588602210470675
8	33	3.552116211378852
8	34	-0.4951005448776016
8	35	0.422931375446538
8	36	-5.3001035084136525
8	37	-9.914599496797567
8	38	-3.676374826726505
8	39	-7.632810010646349
8	40	-5.1243504251965675
8	41	3.2179787642222166
8	42	-1.6242081281064777
8	43	1.5695794050193825
8	44	3.7457346517291903
8	45	3.8182127487294686
8	46	6.025518812070171
8	47	-19.24224544306689
8	48	6.427143559892173
8	49	6.368900456566813
8	50	6.9581156241868705
8	51	-23.04091358276665
8	52	-22.6168701610319
8	53	-22.14512867444858
8	54	-21.869669722606172
8	55	-21.76427117077333
8	56	-21.5496824656145
8	57	-21.06403704010422
8	58	-20.80399401987906
9	1	-7.806436916024467
9	2	-16.227732069932642
9	3	-19.206439183145893
9	4	-11.308399051935602
9	5	-16.345697786357107
9	6	-8.447039059415957
9	7	-12.32254547458653
9	8	-12.249342377457467
9	9	-1.3745403430147363
9	10	-11.521042291172368
9	11	-11.188383383737772
9	12	-8.20692604467147
9	13	-5.802381115015898
9	14	-12.25108810271643
9	15	1.2694790668903657
9	16	-12.342352628172709
9	17	-7.174941704718691
9	18	-14.938527755130416
9	19	0.9377753281553218
9	20	-8.008805574232511
9	21	3.0903108627117817
9	22	3.74181144491293
9	23	5.733930695502956
9	24	5.6632929105202585
9	25	-18.1087858095618
9	26	-1.1020855602429762
9	27	-16.61400367878386
9	28	-0.052754854303479704
9	29	5.047141327142736
9	30	5.847644073544068
9	31	0.8463694395888695
9	32	3.1588602210470675
9	33	6.552116211378852
9	34	-7.495100544877602
9	35	1.422931375446538
9	36	-5.3001035084136525
9	37	-2.9145994967975675
9	38	-0.6763748267265051
9	39	3.367189989353651
9	40	-19.124350425196567
9	41	2.2179787642222166
9	42	-5.624208128106478
9	43	-4.4304205949806175
9	44	-5.25426534827081
9	45	-5.181787251270531
9	46	-15.974481187929829
9	47	-9.242245443066889
9	48	-6.572856440107827
9	49	-1.6310995434331872
9	50	-9.04188437581313
9	51	5.959086417233351
9	52	-8.616870161031901
9	53	6.8548713255514215
9	54	-0.8696697226061723
9	55	7.2357288292266695
9	56	11.450317534385501
9	57	11.935962959895779
9	58	12.19600598012094
10	1	2.1935630839755333
10	2	3.7722679300673576
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115	15	-4.730520933109634
115	16	-4.3423526281727085
115	17	-1.174941704718691
115	18	-10.938527755130416
115	19	-8.062224671844678
115	20	-1.008805574232511
115	21	-0.9096891372882183
115	22	4.74181144491293
115	23	-0.26606930449704436
115	24	-2.3367070894797415
115	25	0.8912141904381983
115	26	2.897914439757024
115	27	-9.61400367878386
115	28	4.94724514569652
115	29	-25.952858672857264
115	30	-25.152355926455932
115	31	-25.15363056041113
115	32	-24.841139778952932
115	33	-24.447883788621148
115	34	-24.4951005448776
115	35	-23.577068624553462
115	36	-25.300103508413653
115	37	-24.914599496797567
115	38	-24.676374826726505
115	39	-24.63281001064635
115	40	-24.124350425196567
115	41	-24.782021235777783
115	42	-24.624208128106478
115	43	-24.430420594980617
115	44	-24.25426534827081
115	45	-24.18178725127053
115	46	-23.97448118792983
115	47	-23.24224544306689
115	48	-23.572856440107827
115	49	-23.631099543433187
115	50	-23.04188437581313
115	51	-23.04091358276665
115	52	-22.6168701610319
115	53	-22.14512867444858
115	54	-21.869669722606172
115	55	-21.76427117077333
115	56	-21.5496824656145
115	57	-21.06403704010422
115	58	-20.80399401987906
116	1	3.1935630839755333
116	2	4.772267930067358
116	3	4.793560816854107
116	4	4.691600948064398
116	5	-3.345697786357107
116	6	3.552960940584043
116	7	3.67745452541347
116	8	3.7506576225425334
116	9	3.6254596569852637
116	10	2.4789577088276324
116	11	4.811616616262228
116	12	6.793073955328531
116	13	4.197618884984102
116	14	1.7489118972835698
116	15	3.2694790668903657
116	16	2.6576473718272915
116	17	-0.17494170471869097
116	18	0.06147224486958436
116	19	-0.06222467184467817
116	20	0.991194425767489
116	21	8.090310862711782
116	22	8.74181144491293
116	23	5.733930695502956
116	24	-0.3367070894797415
116	25	3.8912141904381983
116	26	3.897914439757024
116	27	-1.6140036787838596
116	28	1.9472451456965203
116	29	-12.952858672857264
116	30	4.847644073544068
116	31	-10.15363056041113
116	32	-1.8411397789529325
116	33	-2.447883788621148
116	34	-14.495100544877602
116	35	-3.577068624553462
116	36	0.6998964915863475
116	37	3.0854005032024325
116	38	-0.6763748267265051
116	39	-18.63281001064635
116	40	-0.12435042519656747
116	41	6.217978764222217
116	42	3.3757918718935223
116	43	0.5695794050193825
116	44	5.74573465172919
116	45	-3.1817872512705314
116	46	9.025518812070171
116	47	9.757754556933111
116	48	6.427143559892173
116	49	6.368900456566813
116	50	9.95811562418687
116	51	10.959086417233351
116	52	2.383129838968099
116	53	5.8548713255514215
116	54	3.1303302773938277
116	55	-0.7642711707733305
116	56	1.4503175343855013
116	57	-8.064037040104221
116	58	4.19600598012094
117	1	-0.8064369160244667
117	2	-30.227732069932642
117	3	-29.206439183145893
117	4	-30.308399051935602
117	5	-28.345697786357107
117	6	-29.447039059415957
117	7	-29.32254547458653
117	8	-30.249342377457467
117	9	-30.374540343014736
117	10	-27.521042291172368
117	11	-28.188383383737772
117	12	-29.20692604467147
117	13	-28.802381115015898
117	14	-29.25108810271643
117	15	-29.730520933109634
117	16	-28.34235262817271
117	17	-28.17494170471869
117	18	-27.938527755130416
117	19	-28.062224671844678
117	20	-28.00880557423251
117	21	-27.90968913728822
117	22	-27.25818855508707
117	23	-27.266069304497044
117	24	-27.33670708947974
117	25	-27.1087858095618
117	26	-26.102085560242976
117	27	-25.61400367878386
117	28	-26.05275485430348
117	29	-25.952858672857264
117	30	-25.152355926455932
117	31	-25.15363056041113
117	32	-24.841139778952932
117	33	-24.447883788621148
117	34	-24.4951005448776
117	35	-23.577068624553462
117	36	-25.300103508413653
117	37	-24.914599496797567
117	38	-24.676374826726505
117	39	-24.63281001064635
117	40	-24.124350425196567
117	41	-24.782021235777783
117	42	-24.624208128106478
117	43	-24.430420594980617
117	44	-24.25426534827081
117	45	-24.18178725127053
117	46	-23.97448118792983
117	47	-23.24224544306689
117	48	-23.572856440107827
117	49	-23.631099543433187
117	50	-23.04188437581313
117	51	-23.04091358276665
117	52	-22.6168701610319
117	53	-22.14512867444858
117	54	-21.869669722606172
117	55	-21.76427117077333
117	56	-21.5496824656145
117	57	-21.06403704010422
117	58	-20.80399401987906
119	1	-2.8064369160244667
119	2	-8.227732069932642
119	3	-20.206439183145893
119	4	-9.308399051935602
119	5	-22.345697786357107
119	6	-3.447039059415957
119	7	-17.32254547458653
119	8	-18.249342377457467
119	9	-16.374540343014736
119	10	-2.5210422911723676
119	11	-13.188383383737772
119	12	3.7930739553285306
119	13	-5.802381115015898
119	14	-6.25108810271643
119	15	-6.730520933109634
119	16	-7.3423526281727085
119	17	-1.174941704718691
119	18	-0.9385277551304156
119	19	4.937775328155322
119	20	-0.008805574232511049
119	21	-1.9096891372882183
119	22	-19.25818855508707
119	23	-15.266069304497044
119	24	-1.3367070894797415
119	25	0.8912141904381983
119	26	-0.10208556024297621
119	27	-10.61400367878386
119	28	-0.052754854303479704
119	29	0.04714132714273589
119	30	-3.1523559264559324
119	31	2.8463694395888695
119	32	0.15886022104706754
119	33	-0.44788378862114797
119	34	6.504899455122398
119	35	-14.577068624553462
119	36	-3.3001035084136525
119	37	-0.9145994967975675
119	38	-10.676374826726505
119	39	-1.6328100106463488
119	40	-10.124350425196567
119	41	-9.782021235777783
119	42	1.3757918718935223
119	43	-16.430420594980617
119	44	-5.25426534827081
119	45	-16.18178725127053
119	46	-4.974481187929829
119	47	-7.2422454430668886
119	48	-1.5728564401078273
119	49	2.368900456566813
119	50	2.9581156241868705
119	51	2.959086417233351
119	52	6.383129838968099
119	53	-1.1451286744485785
119	54	2.1303302773938277
119	55	2.2357288292266695
119	56	10.450317534385501
119	57	9.935962959895779
119	58	1.1960059801209404
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	145.0
3	9.0
4	7.0
5	8.0
6	18.0
7	31.0
8	46.0
9	52.0
10	45.0
11	69.0
12	107.0
13	88.0
14	116.0
15	130.0
16	156.0
17	166.0
18	176.0
19	180.0
20	191.0
21	213.0
22	210.0
23	295.0
24	295.0
25	397.0
26	444.0
27	549.0
28	642.0
29	709.0
30	911.0
31	1049.0
32	1320.0
33	1537.0
34	1846.0
35	2295.0
36	3033.0
37	4106.0
38	4262.0
39	3202.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	55.27619178647589	32.83689894751324	2.820389351310449	9.06651991470042
11	30.958932379445553	24.337896402283825	25.889110545504572	18.814060672766043
12	30.456765494943937	16.440806218614572	27.309623718786547	25.792804567654947
13	22.019673935474994	19.010112127674212	30.77663892137305	28.193575015477744
14	20.26552933892825	25.882231547086743	32.41040104560776	21.441838068377244
15	25.682740592969665	28.974341335901492	26.24681846323175	19.09609960789709
16	30.57370846804705	28.16605902180643	23.478021600055033	17.78221091009149
17	29.889248125472932	28.510008942697944	21.438398569168328	20.162344362660797
18	27.63293664442457	28.025039554240905	23.4023526174589	20.939671183875628
19	25.576116117493292	31.020843365206023	23.257893650684462	20.14514686661622
20	26.869367820045404	28.458416454564215	24.516750361147416	20.155465364242968
21	25.88567104629566	26.415353924468597	26.625163376212424	21.07381165302332
22	22.201967393547502	26.329366444245718	28.94682534223017	22.52184081997661
23	24.59241934374355	29.008736327990643	25.957900529682874	20.440943798582925
24	23.82197152094655	30.143771066932658	24.898534773337	21.135722638783793
25	23.832290018573296	32.09052761917865	20.072917383229	24.004264979019055
26	24.374634414891787	32.116436706465265	22.595740288339126	20.91318859030382
27	20.20226342403082	29.482989921227336	21.97722816552578	28.337518489216055
28	21.511887967518838	30.68506348277879	23.899803874341945	23.903244675360426
29	25.98403523259015	27.29493531516653	23.682218552160748	23.038810900082577
30	22.68034140969163	29.178138766519822	25.347604625550662	22.793915198237887
31	28.59060402684564	27.423851316468767	22.409223885733955	21.576320770951643
32	27.227058459209303	29.43949351409008	24.718714516739496	18.614733509961116
33	27.92513847318265	27.058175938349333	25.221041043107306	19.79564454536072
34	24.58711808422791	29.562345169281585	26.24896779521057	19.601568951279937
35	23.641556832650814	33.287449671358274	24.7186757975154	18.352317698475513
36	23.666092943201377	33.11876075731497	24.034423407917384	19.180722891566266
37	27.21514629948365	32.06884681583477	22.15146299483649	18.564543889845094
38	24.110032362459545	33.67417200302968	22.40583901397783	19.809956620532947
39	25.537847234174382	31.788922928642734	21.686000481911123	20.987229355271765
40	24.01969222294901	30.746720831755432	22.625400213447172	22.608186731848384
41	21.931908155186065	34.686219835450444	23.102344314778477	20.279527694585013
42	21.763834842797618	31.46113846895554	23.089638072936395	23.685388615310448
43	21.624879459980715	30.682600909216145	23.367543738806997	24.324975891996143
44	22.971530861647558	27.69458501153224	24.293435230128406	25.040448896691796
45	24.91566265060241	30.309810671256454	22.719449225473323	22.055077452667817
46	22.94370803924944	30.910655878808747	23.48769151316922	22.657944568772596
47	22.31866088034718	30.192188468691878	24.901839222979955	22.58731142798099
48	22.756079079699663	28.99703795550045	26.978714610456706	21.268168354343185
49	25.83801288455576	28.377028284011434	24.342853205636132	21.442105625796675
50	19.928335170893057	29.7271223814774	25.416896361631753	24.927646085997797
51	20.715073022871312	29.57081840727473	28.1310278313585	21.583080738495454
52	22.086375533820085	28.9261606281857	25.35128805620609	23.636175781788126
53	21.077654516640255	29.91111417349962	27.189416385309723	21.821814924550402
54	21.074935400516797	29.88802756244617	27.88630490956072	21.150732127476314
55	21.696130121644437	29.690892174092838	24.332333988076776	24.28064371618595
56	21.94726865414441	30.870239531276926	23.708426675857318	23.47406513872135
57	22.646906772359124	32.772703773910045	22.877821816301914	21.702567637428917
58	22.817357736187226	34.49143487402199	22.26932754282563	20.421879846965155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	47.0
12	94.0
13	311.0
14	528.0
15	625.5
16	591.0
17	459.0
18	336.5
19	214.0
20	191.0
21	168.0
22	135.5
23	104.5
24	106.0
25	112.5
26	119.0
27	171.5
28	230.5
29	237.0
30	291.0
31	345.0
32	358.5
33	372.0
34	385.0
35	554.5
36	711.0
37	601.5
38	492.0
39	604.5
40	717.0
41	675.5
42	672.5
43	711.0
44	846.0
45	981.0
46	952.0
47	1066.0
48	1209.0
49	1095.0
50	981.0
51	1109.5
52	1238.0
53	1236.5
54	1350.5
55	1466.0
56	1663.5
57	1861.0
58	1819.5
59	1811.5
60	1845.0
61	2069.5
62	2294.0
63	2046.0
64	1798.0
65	1559.0
66	1117.5
67	915.0
68	766.5
69	618.0
70	515.0
71	412.0
72	346.0
73	262.5
74	245.0
75	187.5
76	130.0
77	105.5
78	64.0
79	47.0
80	37.0
81	27.0
82	20.0
83	13.0
84	10.5
85	8.0
86	8.0
87	6.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.037834491298067006
27	0.010318497626745545
28	0.037834491298067006
29	0.03439499208915182
30	0.061910985760473276
31	0.06535048496938846
32	0.037834491298067006
33	0.024076494462406273
34	0.03439499208915182
35	0.05159248813372773
36	0.08254798101396436
37	0.08254798101396436
38	0.0963059778496251
39	0.07910848180504919
40	0.0928664786407099
41	0.08598748022287955
42	0.12038247231203136
43	0.13070096993877692
44	0.08598748022287955
45	0.08254798101396436
46	0.09974547705854028
47	0.13757996835660727
48	0.13757996835660727
49	0.16165646281901355
50	0.1719749604457591
51	0.14445896677443762
52	0.13070096993877692
53	0.16509596202792873
54	0.16853546123684393
55	0.189172456490335
56	0.20293045332599574
57	0.20293045332599574
58	0.20980945174382612
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	29074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.4331017403866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.256869579314255	26.84185182637408
2	14.606468347248116	12.395955148930316
3	7.781470373672692	9.905757721675725
4	4.555402447920889	7.7319942216413295
5	2.82888870876226	6.001926119556993
6	1.8805220069709008	4.787782898809933
7	1.2969117289454486	3.8522391139850036
8	0.7619356407554511	2.586503405104217
9	0.59982167463727	2.290706473137511
>10	2.2695955256545353	16.158767283483524
>50	0.11347977628272676	3.4670152025865035
>100	0.048634189835454325	3.9795005847148657
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	362	1.2450987136272957	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	226	0.7773268212148311	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	161	0.5537593726353442	No Hit
TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGATTG	140	0.48152988924812545	No Hit
TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGGCT	136	0.46777189241246475	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	132	0.45401389557680405	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	95	0.3267524248469423	No Hit
TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91	0.3129944280112816	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86	0.29579693196670565	No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.28891793354887524	No Hit
TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCTGC	80	0.27515993671321454	No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	80	0.27515993671321454	No Hit
TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATG	76	0.26140193987755383	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	69	0.23732544541514755	No Hit
TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTTAC	62	0.2132489509527413	No Hit
TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59	0.20293045332599574	No Hit
TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	59	0.20293045332599574	No Hit
TTCCGCGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA	58	0.19949095411708057	No Hit
TTCCGCGGGACTTCATTTTCTTTGGAAGAAAATGATGTCTAAAGAACATATAGAGGCA	56	0.1926119556992502	No Hit
TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTT	53	0.18229345807250463	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	49	0.16853546123684393	No Hit
TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA	49	0.16853546123684393	No Hit
TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTGCA	49	0.16853546123684393	No Hit
TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTAT	48	0.16509596202792873	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAA	45	0.1547774644011832	No Hit
TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCTGT	44	0.15133796519226803	No Hit
TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43	0.14789846598335282	No Hit
TTCCGCGGGGTGGGTCATCAGCCCATGCCTCACCCTGGCAGTGTGCTCTAACTGAGGC	42	0.14445896677443762	No Hit
TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	40	0.13757996835660727	No Hit
TTCCGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT	39	0.1341404691476921	No Hit
TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.1341404691476921	No Hit
TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCACCA	39	0.1341404691476921	No Hit
TTCCGCGGGGATGAAAGGAAAAAGAATGCCAAAATCAGCAGCCTCCTGGAGGAGCAGT	38	0.13070096993877692	No Hit
TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	38	0.13070096993877692	No Hit
TTCCGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	37	0.12726147072986171	No Hit
TTCCGCGGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTTTTCAG	37	0.12726147072986171	No Hit
TTCCGCGGGGGTGCTATGTGGGGACATTGAGACAAACCAATCTTTTGTACTTCCACTT	36	0.12382197152094655	No Hit
TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTTTC	36	0.12382197152094655	No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTCTTTCAG	35	0.12038247231203136	No Hit
TTCCGCGGGGTTGTATGATGCCACTTTCCATGTGTATGTGATACCACTGTACATGTGT	34	0.11694297310311619	No Hit
TTCCGCGGGATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGAT	32	0.11006397468528582	No Hit
TTCCGCGGGAGTGACAAATTATATGTTATAGAAGGTCCAATGTGGGGATAGTGTGTGG	32	0.11006397468528582	No Hit
TTCCGCGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGA	31	0.10662447547637065	No Hit
TTCCGCGGGGGGTCCTAACAGTCTTCACTGCCATCCCCAAGGCCATCACCTCTGGTTT	31	0.10662447547637065	No Hit
TTCCGCGGGATTTTTGAAATAAATTTTTTAATTTATAGAGTATTTCTGAGGGGTGGGG	30	0.10318497626745546	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCTGACTGCTCTTCCGA	30	0.10318497626745546	No Hit
TTCCGCGGGAATTTGGGTTCCTTAGCCATTCCCAGAGAGGGGGAGGTGAGGCCCAGAA	30	0.10318497626745546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.010318497626745545	0.0	0.0	0.0	0.0
11	0.010318497626745545	0.0	0.0	0.0	0.0
12	0.010318497626745545	0.0	0.0	0.0	0.0
13	0.010318497626745545	0.0	0.0	0.0	0.0
14	0.027515993671321456	0.0	0.0	0.0	0.0
15	0.027515993671321456	0.0	0.0	0.0	0.0
16	0.027515993671321456	0.0	0.0	0.0	0.0
17	0.027515993671321456	0.0	0.0	0.0	0.0
18	0.027515993671321456	0.0	0.0	0.0	0.0
19	0.030955492880236638	0.0	0.0	0.0	0.0
20	0.03439499208915182	0.0	0.0	0.0	0.0
21	0.03439499208915182	0.0	0.0	0.0	0.0
22	0.03439499208915182	0.0	0.0	0.0	0.0
23	0.03439499208915182	0.0	0.0	0.0	0.0
24	0.05159248813372773	0.0	0.0	0.0	0.0
25	0.05503198734264291	0.0	0.0	0.0	0.0
26	0.058471486551558094	0.0	0.0	0.0	0.0
27	0.061910985760473276	0.0	0.0	0.0	0.0
28	0.113503473894201	0.0	0.0	0.0	0.0
29	0.12382197152094655	0.0	0.0	0.0	0.0
30	0.13757996835660727	0.0	0.0	0.0	0.0
31	0.13757996835660727	0.0	0.0	0.0	0.0
32	0.13757996835660727	0.0	0.0	0.0	0.0
33	0.13757996835660727	0.0	0.0	0.0	0.0
34	0.13757996835660727	0.0	0.0	0.0	0.0
35	0.1685354612368439	0.0	0.0	0.0	0.0
36	0.1719749604457591	0.0	0.0	0.0	0.0
37	0.1719749604457591	0.0	0.0	0.0	0.0
38	0.17541445965467428	0.0	0.0	0.0	0.0
39	0.17885395886358946	0.0	0.0	0.0	0.0
40	0.17885395886358946	0.0	0.0	0.0	0.0
41	0.17885395886358946	0.0	0.0	0.0	0.0
42	0.17885395886358946	0.0	0.0	0.0	0.0
43	0.17885395886358946	0.0	0.0	0.0	0.0
44	0.17885395886358946	0.0	0.0	0.0	0.0
45	0.17885395886358946	0.0	0.0	0.0	0.0
46	0.18573295728141984	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTTAC	25	1.5974561E-5	52.09672	47
GTGTTGG	15	0.007097281	52.096718	50
TTACCTT	15	0.007097281	52.096718	39
CCTAATA	20	3.3607965E-4	52.096718	39
TGTTACT	15	0.007097281	52.096718	48
ATTACCT	15	0.007097281	52.096718	38
CACCTGA	15	0.007097281	52.096718	40
CTAATAT	20	3.3607965E-4	52.096718	40
ACCTTTA	15	0.007097281	52.096718	41
AGACTCT	15	0.007097281	52.096718	39
ACCTAAT	20	3.3607965E-4	52.096718	38
TGGTGTT	15	0.007097281	52.096718	48
GTTACTT	15	0.007097281	52.096718	49
GACTTCT	20	3.3607965E-4	52.096718	44
ACAGTGG	20	3.389647E-4	52.0069	27
TCGAACT	20	3.389647E-4	52.0069	21
TTCGAAC	20	3.389647E-4	52.0069	20
TGTTAAG	20	3.389647E-4	52.0069	31
GCTGTTA	20	3.389647E-4	52.0069	29
CGAACTT	20	3.389647E-4	52.0069	22
>>END_MODULE