SRR212870_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212870_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30527
Sequences flagged as poor quality	0
Sequence length	58
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	343	1.1235955056179776	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.8287745274674877	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	188	0.6158482654699119	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	177	0.5798145903626298	No Hit
TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGATTG	150	0.4913682969174829	No Hit
TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGGCT	136	0.44550725587185114	No Hit
TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCTGC	111	0.36361253971893737	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	104	0.34068201919612146	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83	0.27189045762767383	No Hit
TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.2686146689815573	No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	79	0.2587873030432077	No Hit
TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATG	75	0.24568414845874145	No Hit
TTCCGCGGGACTTCATTTTCTTTGGAAGAAAATGATGTCTAAAGAACATATAGAGGCA	74	0.2424083598126249	No Hit
TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72	0.2358567825203918	No Hit
TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	72	0.2358567825203918	No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70	0.2293052052281587	No Hit
TTCCGCGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA	70	0.2293052052281587	No Hit
TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA	62	0.20309889605922624	No Hit
TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	59	0.1932715301208766	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	58	0.18999574147476006	No Hit
TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTTAC	55	0.18016837553641038	No Hit
TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTGCA	51	0.16706522095194418	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	49	0.16051364365971107	No Hit
TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.15396206636747797	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	46	0.1506862777213614	No Hit
TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTT	46	0.1506862777213614	No Hit
TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCTGT	46	0.1506862777213614	No Hit
TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.14741048907524487	No Hit
TTCCGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT	44	0.14413470042912832	No Hit
TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCACCA	42	0.13758312313689522	No Hit
TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	42	0.13758312313689522	No Hit
TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTTTC	41	0.13430733449077864	No Hit
TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTAT	39	0.12775575719854554	No Hit
TTCCGCGGGGACTTCTCTATTACAAAGATAAGAAACGGTCCCACAAATAAAAAAATAA	35	0.11465260261407935	No Hit
TTCCGCGGGGGTGCTATGTGGGGACATTGAGACAAACCAATCTTTTGTACTTCCACTT	34	0.11137681396796278	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAA	34	0.11137681396796278	No Hit
TTCCGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	33	0.10810102532184623	No Hit
TTCCGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	33	0.10810102532184623	No Hit
TTCCGCGGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTTTTCAG	33	0.10810102532184623	No Hit
TTCCGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	33	0.10810102532184623	No Hit
TTCCGCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC	32	0.10482523667572968	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA	32	0.10482523667572968	No Hit
TTCCGCGGGAGTGACAAATTATATGTTATAGAAGGTCCAATGTGGGGATAGTGTGTGG	32	0.10482523667572968	No Hit
TTCCGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.10154944802961312	No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCACTTCCAG	31	0.10154944802961312	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAAACC	25	1.5989297E-5	52.098846	43
ATTGGCT	20	3.3627253E-4	52.098846	25
TTCTGCC	25	1.5989297E-5	52.098846	29
GCTGTTG	20	3.3627253E-4	52.098846	49
TATTTGC	20	3.3627253E-4	52.098846	29
TACCATG	20	3.3627253E-4	52.098846	31
AGTTCTG	25	1.5989297E-5	52.098846	27
CAAACCT	25	1.5989297E-5	52.098846	44
TTTGCAA	20	3.3627253E-4	52.098846	31
GTTGCCC	20	3.3627253E-4	52.098846	52
AGCCCTC	20	3.3627253E-4	52.098846	41
TGAGACT	15	0.0070994375	52.098843	35
ACACGAG	15	0.0070994375	52.098843	38
ACATGCA	15	0.0070994375	52.098843	45
TGCGCCA	15	0.0070994375	52.098843	44
CCTGCGC	15	0.0070994375	52.098843	42
CACAAGA	15	0.0070994375	52.098843	37
CACCGAC	15	0.0070994375	52.098843	40
AAATACC	15	0.0070994375	52.098843	39
CACGAGT	15	0.0070994375	52.098843	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content