##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212870_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30527
Sequences flagged as poor quality	0
Sequence length	58
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.55147901857372	39.0	38.0	39.0	33.0	40.0
2	37.25823041897337	39.0	38.0	39.0	33.0	40.0
3	37.06823467749861	39.0	36.0	39.0	33.0	40.0
4	37.36279359255741	39.0	38.0	39.0	33.0	40.0
5	36.286860811740425	38.0	35.0	39.0	31.0	40.0
6	36.444983129688474	38.0	35.0	39.0	31.0	40.0
7	36.95407344318144	39.0	36.0	39.0	33.0	40.0
8	37.22255708061716	39.0	38.0	39.0	33.0	40.0
9	37.22953451043339	39.0	38.0	39.0	33.0	40.0
10	35.26904052150555	38.0	33.0	39.0	28.0	40.0
11	35.40701673927998	38.0	35.0	39.0	28.0	40.0
12	35.846136207291906	38.0	35.0	39.0	29.0	40.0
13	35.839388082680905	38.0	35.0	39.0	29.0	40.0
14	35.89301274281783	38.0	35.0	39.0	30.0	40.0
15	35.979919415599305	38.0	35.0	39.0	30.0	40.0
16	35.562321878992364	38.0	35.0	39.0	29.0	40.0
17	35.26782847970649	38.0	33.0	39.0	28.0	40.0
18	35.261342418187176	38.0	33.0	39.0	28.0	40.0
19	35.1186818226488	38.0	33.0	39.0	28.0	40.0
20	34.990107118288726	38.0	33.0	39.0	28.0	40.0
21	34.980803878533756	38.0	33.0	39.0	28.0	40.0
22	34.85435843679366	38.0	33.0	39.0	27.0	40.0
23	34.68103645952763	38.0	33.0	39.0	27.0	40.0
24	34.60238477413437	38.0	33.0	39.0	27.0	40.0
25	34.588429914501916	38.0	33.0	39.0	27.0	40.0
26	34.112785403085795	37.0	33.0	39.0	25.0	40.0
27	33.980181478690994	37.0	33.0	39.0	25.0	40.0
28	33.65889212827988	36.0	32.0	39.0	24.0	40.0
29	33.72185278605824	37.0	32.0	39.0	25.0	40.0
30	33.380679398565206	36.0	32.0	39.0	23.0	40.0
31	32.977331542568876	36.0	31.0	38.0	22.0	40.0
32	32.753464146493265	36.0	31.0	38.0	22.0	40.0
33	32.596357323025515	36.0	31.0	38.0	21.0	40.0
34	32.15091558292659	35.0	30.0	38.0	19.0	39.0
35	31.99095882333672	35.0	30.0	38.0	19.0	39.0
36	32.90523143446785	36.0	32.0	39.0	21.0	40.0
37	32.79568906214171	36.0	32.0	39.0	21.0	40.0
38	32.560258132145314	36.0	31.0	39.0	20.0	40.0
39	32.595047007567075	36.0	31.0	39.0	20.0	40.0
40	32.33891309332722	36.0	31.0	39.0	18.0	40.0
41	32.54410849411996	36.0	32.0	39.0	18.0	40.0
42	32.398696236118845	36.0	31.0	39.0	18.0	40.0
43	32.27156287876306	36.0	31.0	39.0	17.0	40.0
44	32.062927899891896	36.0	31.0	39.0	16.0	40.0
45	31.945097782291086	36.0	31.0	39.0	15.0	40.0
46	31.416188947489108	36.0	30.0	39.0	7.0	40.0
47	31.20460575883644	36.0	30.0	39.0	2.0	40.0
48	31.042257673534902	35.0	30.0	38.0	2.0	39.0
49	30.932354964457694	35.0	30.0	38.0	2.0	40.0
50	30.601827890064534	35.0	29.0	38.0	2.0	39.0
51	30.193828414190715	35.0	29.0	38.0	2.0	39.0
52	30.077931011891113	35.0	29.0	38.0	2.0	39.0
53	29.834703704916958	35.0	28.0	38.0	2.0	39.0
54	29.610869066727815	35.0	28.0	38.0	2.0	39.0
55	29.471549775608477	35.0	28.0	38.0	2.0	39.0
56	29.242113538834474	35.0	28.0	38.0	2.0	39.0
57	28.84937923805156	35.0	27.0	38.0	2.0	39.0
58	28.561666721263144	35.0	26.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	-1.8078378861291853
2	2	-0.7061192147898936
2	3	-2.973081256731028
2	4	-0.7798164743982454
2	5	-2.017802909334442
2	6	-4.620429002014902
2	7	-13.86724843377229
2	8	-4.960311003330823
2	9	-2.876734096587974
2	10	-6.043380926532521
2	11	-3.1582989365002163
2	12	-2.9330598235276923
2	13	3.257274317241631
2	14	2.9703891794539032
2	15	3.2380663624684374
2	16	-11.953747245626385
2	17	-9.345226019315731
2	18	-7.8214024374689615
2	19	-12.183662792664581
2	20	1.5622914177347695
2	21	-10.608593496384938
2	22	0.7180841582989181
2	23	-13.179432002391767
2	24	-6.645793338646758
2	25	3.3863613483724
2	26	-19.637823581039836
2	27	-0.40844366637735163
2	28	-0.10600096832174444
2	29	-7.176974529301482
2	30	4.892974742578158
2	31	8.962234099142563
2	32	5.748839915268306
2	33	3.4467943788285034
2	34	4.445445131823504
2	35	-0.5322049645338396
2	36	-2.419228447881803
2	37	5.942055728076504
2	38	0.8045742228278279
2	39	0.01924412790216934
2	40	-7.658222799345417
2	41	2.2747285891586344
2	42	5.397926418833414
2	43	2.9275028463274886
2	44	5.937389475107363
2	45	8.172195541047351
2	46	9.667223772224656
2	47	4.889980821435341
2	48	4.652470221708814
2	49	-8.56733249721292
2	50	3.7150681470821247
2	51	4.965615907145249
2	52	8.594805681725731
2	53	11.405979881335146
2	54	4.990667070888158
2	55	6.116364492667245
2	56	8.627962061034001
2	57	-0.9360773573361136
2	58	9.449166207251977
3	1	2.1921621138708147
3	2	3.2938807852101064
3	3	1.026918743268972
3	4	3.2201835256017546
3	5	2.982197090665558
3	6	3.3795709979850983
3	7	0.13275156622771078
3	8	2.0396889966691774
3	9	2.123265903412026
3	10	0.9566190734674791
3	11	-2.1582989365002163
3	12	-2.9330598235276923
3	13	-6.742725682758369
3	14	-3.0296108205460968
3	15	3.2380663624684374
3	16	1.046252754373615
3	17	-9.345226019315731
3	18	-6.8214024374689615
3	19	-8.183662792664581
3	20	-7.4377085822652305
3	21	-6.6085934963849375
3	22	-2.281915841701082
3	23	-18.179432002391767
3	24	-2.645793338646758
3	25	0.3863613483724002
3	26	-2.637823581039836
3	27	1.5915563336226484
3	28	-2.1060009683217444
3	29	-0.17697452930148216
3	30	-6.107025257421842
3	31	-5.037765900857437
3	32	1.7488399152683058
3	33	-5.553205621171497
3	34	-13.554554868176496
3	35	-2.5322049645338396
3	36	2.580771552118197
3	37	-4.057944271923496
3	38	2.804574222827828
3	39	3.0192441279021693
3	40	-0.6582227993454168
3	41	0.2747285891586344
3	42	4.397926418833414
3	43	-6.072497153672511
3	44	2.937389475107363
3	45	5.172195541047351
3	46	4.667223772224656
3	47	4.889980821435341
3	48	7.652470221708814
3	49	-1.5673324972129201
3	50	5.715068147082125
3	51	5.965615907145249
3	52	-1.405194318274269
3	53	5.405979881335146
3	54	5.990667070888158
3	55	-3.8836355073327553
3	56	3.627962061034001
3	57	8.063922642663886
3	58	2.4491662072519773
5	1	-2.8078378861291853
5	2	-21.706119214789894
5	3	-22.973081256731028
5	4	-22.779816474398245
5	5	-22.017802909334442
5	6	-16.6204290020149
5	7	-15.86724843377229
5	8	-16.960311003330823
5	9	-16.876734096587974
5	10	-15.043380926532521
5	11	-1.1582989365002163
5	12	-1.9330598235276923
5	13	-1.742725682758369
5	14	-2.0296108205460968
5	15	-1.7619336375315626
5	16	-29.953747245626385
5	17	-29.34522601931573
5	18	-29.82140243746896
5	19	-29.18366279266458
5	20	-29.43770858226523
5	21	-28.608593496384938
5	22	-28.281915841701082
5	23	-28.179432002391767
5	24	-27.645793338646758
5	25	-27.6136386516276
5	26	-26.637823581039836
5	27	-26.40844366637735
5	28	-26.106000968321744
5	29	-26.176974529301482
5	30	-26.107025257421842
5	31	-25.037765900857437
5	32	-25.251160084731694
5	33	-24.553205621171497
5	34	-24.554554868176496
5	35	-24.53220496453384
5	36	-25.419228447881803
5	37	-25.057944271923496
5	38	-25.195425777172172
5	39	-24.98075587209783
5	40	-24.658222799345417
5	41	-25.725271410841366
5	42	-25.602073581166586
5	43	-25.07249715367251
5	44	-25.062610524892637
5	45	-24.82780445895265
5	46	-24.332776227775344
5	47	-24.11001917856466
5	48	-24.347529778291186
5	49	-23.56733249721292
5	50	-23.284931852917875
5	51	-23.03438409285475
5	52	-22.40519431827427
5	53	-22.594020118664854
5	54	-22.009332929111842
5	55	-21.883635507332755
5	56	-22.372037938966
5	57	-21.936077357336114
5	58	-21.550833792748023
6	1	-11.807837886129185
6	2	-26.706119214789894
6	3	-21.973081256731028
6	4	-26.779816474398245
6	5	-25.017802909334442
6	6	-23.6204290020149
6	7	-18.86724843377229
6	8	-19.960311003330823
6	9	-23.876734096587974
6	10	-14.043380926532521
6	11	-16.158298936500216
6	12	-0.9330598235276923
6	13	-12.742725682758369
6	14	-11.029610820546097
6	15	-10.761933637531563
6	16	-8.953747245626385
6	17	-6.3452260193157315
6	18	-6.8214024374689615
6	19	-6.183662792664581
6	20	-6.4377085822652305
6	21	-7.6085934963849375
6	22	-10.281915841701082
6	23	0.820567997608233
6	24	-0.645793338646758
6	25	-6.6136386516276
6	26	-5.637823581039836
6	27	-1.4084436663773516
6	28	-5.106000968321744
6	29	-5.176974529301482
6	30	4.892974742578158
6	31	1.962234099142563
6	32	-9.251160084731694
6	33	-13.553205621171497
6	34	-13.554554868176496
6	35	2.4677950354661604
6	36	-8.419228447881803
6	37	-15.057944271923496
6	38	-10.195425777172172
6	39	-14.98075587209783
6	40	-4.658222799345417
6	41	-5.725271410841366
6	42	-8.602073581166586
6	43	-9.072497153672511
6	44	-9.062610524892637
6	45	-10.827804458952649
6	46	4.667223772224656
6	47	1.8899808214353406
6	48	4.652470221708814
6	49	0.4326675027870799
6	50	-6.284931852917875
6	51	-5.034384092854751
6	52	6.594805681725731
6	53	6.405979881335146
6	54	3.9906670708881578
6	55	7.116364492667245
6	56	11.627962061034001
6	57	7.063922642663886
6	58	11.449166207251977
7	1	-32.807837886129185
7	2	-31.706119214789894
7	3	-30.973081256731028
7	4	-31.779816474398245
7	5	-30.017802909334442
7	6	-30.6204290020149
7	7	-30.86724843377229
7	8	-31.960311003330823
7	9	-31.876734096587974
7	10	-29.04338092653252
7	11	-30.158298936500216
7	12	-30.933059823527692
7	13	-30.74272568275837
7	14	-31.029610820546097
7	15	-30.761933637531563
7	16	-29.953747245626385
7	17	-29.34522601931573
7	18	-29.82140243746896
7	19	-29.18366279266458
7	20	-29.43770858226523
7	21	-28.608593496384938
7	22	-28.281915841701082
7	23	-28.179432002391767
7	24	-27.645793338646758
7	25	-27.6136386516276
7	26	-26.637823581039836
7	27	-26.40844366637735
7	28	-26.106000968321744
7	29	-26.176974529301482
7	30	-26.107025257421842
7	31	-25.037765900857437
7	32	-25.251160084731694
7	33	-24.553205621171497
7	34	-24.554554868176496
7	35	-24.53220496453384
7	36	-25.419228447881803
7	37	-25.057944271923496
7	38	-25.195425777172172
7	39	-24.98075587209783
7	40	-24.658222799345417
7	41	-25.725271410841366
7	42	-25.602073581166586
7	43	-25.07249715367251
7	44	-25.062610524892637
7	45	-24.82780445895265
7	46	-24.332776227775344
7	47	-24.11001917856466
7	48	-24.347529778291186
7	49	-23.56733249721292
7	50	-23.284931852917875
7	51	-23.03438409285475
7	52	-22.40519431827427
7	53	-22.594020118664854
7	54	-22.009332929111842
7	55	-21.883635507332755
7	56	-22.372037938966
7	57	-21.936077357336114
7	58	-21.550833792748023
9	1	-3.8078378861291853
9	2	-19.706119214789894
9	3	-15.973081256731028
9	4	-16.779816474398245
9	5	-18.017802909334442
9	6	-12.620429002014902
9	7	-11.86724843377229
9	8	-10.960311003330823
9	9	-14.876734096587974
9	10	-12.043380926532521
9	11	1.8417010634997837
9	12	-1.9330598235276923
9	13	1.257274317241631
9	14	0.9703891794539032
9	15	1.2380663624684374
9	16	2.046252754373615
9	17	-0.3452260193157315
9	18	2.1785975625310385
9	19	2.816337207335419
9	20	-0.43770858226523046
9	21	0.39140650361506246
9	22	0.7180841582989181
9	23	0.820567997608233
9	24	1.354206661353242
9	25	1.3863613483724002
9	26	2.362176418960164
9	27	2.5915563336226484
9	28	2.8939990316782556
9	29	-11.176974529301482
9	30	-11.107025257421842
9	31	-11.037765900857437
9	32	-11.251160084731694
9	33	-15.553205621171497
9	34	-3.5545548681764956
9	35	-8.53220496453384
9	36	-11.419228447881803
9	37	-11.057944271923496
9	38	-11.195425777172172
9	39	-10.98075587209783
9	40	-10.658222799345417
9	41	1.2747285891586344
9	42	-5.602073581166586
9	43	-7.072497153672511
9	44	-4.062610524892637
9	45	8.172195541047351
9	46	-6.332776227775344
9	47	4.889980821435341
9	48	-5.347529778291186
9	49	-4.56733249721292
9	50	-4.284931852917875
9	51	5.965615907145249
9	52	6.594805681725731
9	53	6.405979881335146
9	54	6.990667070888158
9	55	7.116364492667245
9	56	6.627962061034001
9	57	5.063922642663886
9	58	7.449166207251977
10	1	2.1921621138708147
10	2	3.2938807852101064
10	3	3.026918743268972
10	4	2.2201835256017546
10	5	0.9821970906655579
10	6	0.3795709979850983
10	7	0.13275156622771078
10	8	1.0396889966691774
10	9	1.123265903412026
10	10	1.956619073467479
10	11	-7.158298936500216
10	12	0.06694017647230766
10	13	2.257274317241631
10	14	-3.0296108205460968
10	15	0.23806636246843738
10	16	-1.953747245626385
10	17	-3.3452260193157315
10	18	1.1785975625310385
10	19	-4.183662792664581
10	20	1.5622914177347695
10	21	-2.6085934963849375
10	22	-3.281915841701082
10	23	-7.179432002391767
10	24	3.354206661353242
10	25	-7.6136386516276
10	26	-5.637823581039836
10	27	-26.40844366637735
10	28	-8.106000968321744
10	29	-8.176974529301482
10	30	-9.107025257421842
10	31	-10.037765900857437
10	32	-14.251160084731694
10	33	-10.553205621171497
10	34	-9.554554868176496
10	35	-0.5322049645338396
10	36	5.580771552118197
10	37	2.942055728076504
10	38	5.804574222827828
10	39	3.0192441279021693
10	40	3.341777200654583
10	41	4.274728589158634
10	42	2.397926418833414
10	43	4.927502846327489
10	44	-3.062610524892637
10	45	5.172195541047351
10	46	5.667223772224656
10	47	5.889980821435341
10	48	9.652470221708814
10	49	8.43266750278708
10	50	-2.2849318529178753
10	51	9.96561590714525
10	52	10.594805681725731
10	53	10.405979881335146
10	54	10.990667070888158
10	55	5.116364492667245
10	56	8.627962061034001
10	57	9.063922642663886
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116	54	5.990667070888158
116	55	-0.8836355073327553
116	56	8.627962061034001
116	57	8.063922642663886
116	58	9.449166207251977
117	1	-32.807837886129185
117	2	-31.706119214789894
117	3	-30.973081256731028
117	4	-31.779816474398245
117	5	-30.017802909334442
117	6	-30.6204290020149
117	7	-30.86724843377229
117	8	-31.960311003330823
117	9	-31.876734096587974
117	10	-29.04338092653252
117	11	-30.158298936500216
117	12	-30.933059823527692
117	13	-30.74272568275837
117	14	-31.029610820546097
117	15	-30.761933637531563
117	16	-29.953747245626385
117	17	-29.34522601931573
117	18	-29.82140243746896
117	19	-29.18366279266458
117	20	-29.43770858226523
117	21	-28.608593496384938
117	22	-28.281915841701082
117	23	-28.179432002391767
117	24	-27.645793338646758
117	25	-27.6136386516276
117	26	-26.637823581039836
117	27	-26.40844366637735
117	28	-26.106000968321744
117	29	-26.176974529301482
117	30	-26.107025257421842
117	31	-25.037765900857437
117	32	-25.251160084731694
117	33	-24.553205621171497
117	34	-24.554554868176496
117	35	-24.53220496453384
117	36	-25.419228447881803
117	37	-25.057944271923496
117	38	-25.195425777172172
117	39	-24.98075587209783
117	40	-24.658222799345417
117	41	-25.725271410841366
117	42	-25.602073581166586
117	43	-25.07249715367251
117	44	-25.062610524892637
117	45	-24.82780445895265
117	46	-24.332776227775344
117	47	-24.11001917856466
117	48	-24.347529778291186
117	49	-23.56733249721292
117	50	-23.284931852917875
117	51	-23.03438409285475
117	52	-22.40519431827427
117	53	-22.594020118664854
117	54	-22.009332929111842
117	55	-21.883635507332755
117	56	-22.372037938966
117	57	-21.936077357336114
117	58	-21.550833792748023
119	1	-14.807837886129185
119	2	-13.706119214789894
119	3	-13.973081256731028
119	4	-13.779816474398245
119	5	-14.517802909334442
119	6	-12.620429002014902
119	7	-15.36724843377229
119	8	-13.960311003330823
119	9	-13.876734096587974
119	10	-11.043380926532521
119	11	-12.158298936500216
119	12	-14.433059823527692
119	13	-12.742725682758369
119	14	-14.029610820546097
119	15	-16.261933637531563
119	16	-14.953747245626385
119	17	-11.345226019315731
119	18	-11.821402437468961
119	19	-11.183662792664581
119	20	-13.93770858226523
119	21	-12.108593496384938
119	22	-10.281915841701082
119	23	-11.679432002391767
119	24	-16.645793338646758
119	25	-9.6136386516276
119	26	-19.137823581039836
119	27	-14.908443666377352
119	28	-15.606000968321744
119	29	-17.676974529301482
119	30	-13.107025257421842
119	31	-18.037765900857437
119	32	-16.251160084731694
119	33	-20.053205621171497
119	34	-18.054554868176496
119	35	-17.53220496453384
119	36	-20.419228447881803
119	37	-18.057944271923496
119	38	-20.195425777172172
119	39	-13.98075587209783
119	40	-18.658222799345417
119	41	-8.725271410841366
119	42	-7.602073581166586
119	43	-7.072497153672511
119	44	-11.562610524892637
119	45	-7.827804458952649
119	46	-7.832776227775344
119	47	-7.610019178564659
119	48	-7.347529778291186
119	49	-7.06733249721292
119	50	-5.284931852917875
119	51	-9.53438409285475
119	52	-6.405194318274269
119	53	-6.594020118664854
119	54	-7.009332929111842
119	55	-5.883635507332755
119	56	-4.372037938965999
119	57	-6.436077357336114
119	58	-5.050833792748023
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	123.0
3	3.0
4	13.0
5	10.0
6	19.0
7	30.0
8	39.0
9	56.0
10	68.0
11	93.0
12	120.0
13	111.0
14	134.0
15	156.0
16	164.0
17	163.0
18	206.0
19	226.0
20	203.0
21	246.0
22	291.0
23	304.0
24	344.0
25	408.0
26	542.0
27	616.0
28	699.0
29	868.0
30	940.0
31	1088.0
32	1336.0
33	1689.0
34	2000.0
35	2488.0
36	3183.0
37	4187.0
38	4239.0
39	3089.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	55.40341337176925	32.61047597209028	2.7713171946146034	9.214793461525863
11	31.142922658630066	24.663412716611525	25.639597733154257	18.554066891604155
12	30.605693320666948	16.166016968585186	27.48714252956399	25.74114718118387
13	22.638975333311496	18.88492154486193	30.025878730304324	28.450224391522262
14	19.913519179742522	26.44216595145281	32.75133488387329	20.892979984931372
15	25.623218789923673	28.715563271857697	26.376650178530483	19.284567759688144
16	31.195335276967928	27.569037245716903	23.52671405640908	17.708913420906082
17	29.92105349362859	28.51574016444459	21.91175025387362	19.651456088053198
18	27.60868852996101	27.933034105428696	23.369262523343053	21.089014841267243
19	25.55115143970911	31.113440560815015	23.294133062534804	20.04127493694107
20	26.448717528745046	28.813836931241198	24.679791659842106	20.057653880171653
21	25.665804042323188	26.520784878959606	27.251285747043603	20.5621253316736
22	22.783110033740623	26.258721787270282	28.669702230812067	22.288465948177024
23	24.604448520981425	28.856422183640714	26.478199626560095	20.060929668817767
24	23.870523867247652	30.183795826098354	24.630606427939586	21.315073878714415
25	23.864120286959086	32.23376027778688	19.98231074131097	23.919808693943068
26	24.117936117936118	32.17690417690417	22.774774774774777	20.93038493038493
27	19.991480437774428	29.477685300478406	21.898551674421654	28.632282587325513
28	21.869471201100847	30.48620667059826	23.641963174103925	24.00235895419697
29	26.087668719695976	27.388284628489057	23.479884680906828	23.04416197090814
30	23.176767511226195	28.493231505457405	25.926775705529515	22.40322527778688
31	28.533988533988534	27.603603603603606	22.67977067977068	21.182637182637183
32	27.44577681672236	29.34932180066837	24.759190092392373	18.44571129021689
33	27.635374328045103	26.412744198243082	25.83584633538744	20.116035138324374
34	24.061086714295076	29.779773218850362	26.325621026414105	19.833519040440454
35	23.32066321515171	33.29838128317714	24.97870109443607	18.402254407235073
36	23.942830918507834	33.649118206254506	23.5855241591818	18.822526716055858
37	27.15292574987707	32.437305359777085	21.88821504671365	18.52155384363219
38	23.719588169716047	33.815332152928065	22.453931405338054	20.011148272017838
39	25.807615361910074	31.72083565642321	21.10130858286052	21.370240398806207
40	23.821543302956798	30.872615223234774	22.343145610699537	22.962695863108898
41	21.19294333683106	34.69307450157398	23.04564533053515	21.06833683105981
42	21.767213114754096	31.160655737704918	23.062295081967214	24.00983606557377
43	21.7854566913645	30.873385351780215	23.16569405284899	24.175463904006296
44	23.109464156883323	28.018626615071817	24.322817603462976	24.549091624581884
45	25.395107875926293	30.10361335169519	22.34244868515968	22.158830087218835
46	22.38399737661912	31.43794064600754	23.364485981308412	22.813575996064927
47	22.124532510990093	30.3687422085165	24.762154714257594	22.74457056623581
48	22.890696758955517	29.038183965358876	26.692691247867735	21.378428027817872
49	25.738936456385524	28.77013417314569	24.492339992782863	20.99858937768592
50	19.93962660366834	29.897955835548117	24.894182498277388	25.26823506250615
51	20.660804514731936	29.94290963974014	27.866001706148698	21.530284139379223
52	21.841267758128545	29.295580563666785	25.27970077758457	23.583450900620097
53	21.223753280839894	29.75721784776903	27.221128608923884	21.79790026246719
54	20.863828809609767	30.181495946699926	27.358955003446127	21.59572024024418
55	21.25340599455041	29.897245658382847	23.9650700896228	24.884278257443945
56	21.687300909658138	31.296180749400676	23.670815408360973	23.345702932580213
57	22.7801129646657	33.038880861683964	22.366347037961383	21.814659135688952
58	22.940539120727582	34.99359753094527	21.850477722691007	20.21538562563614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	49.5
12	99.0
13	325.5
14	552.0
15	675.0
16	613.5
17	429.0
18	319.5
19	210.0
20	193.5
21	177.0
22	143.5
23	117.5
24	125.0
25	128.5
26	132.0
27	185.5
28	242.0
29	245.0
30	314.0
31	383.0
32	399.0
33	415.0
34	417.0
35	564.5
36	710.0
37	628.0
38	546.0
39	675.0
40	804.0
41	731.5
42	685.5
43	712.0
44	852.0
45	992.0
46	976.0
47	1110.5
48	1261.0
49	1150.0
50	1039.0
51	1169.5
52	1300.0
53	1301.0
54	1393.5
55	1485.0
56	1680.0
57	1875.0
58	1867.0
59	1988.5
60	2118.0
61	2252.5
62	2387.0
63	2156.0
64	1925.0
65	1637.5
66	1155.5
67	961.0
68	812.0
69	663.0
70	535.0
71	407.0
72	359.5
73	275.5
74	239.0
75	189.0
76	139.0
77	112.0
78	67.0
79	49.0
80	39.0
81	29.0
82	20.5
83	12.0
84	10.5
85	6.0
86	3.0
87	1.5
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.01310315458446621
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.01310315458446621
25	0.0
26	0.006551577292233105
27	0.029482097815048975
28	0.01637894323058276
29	0.009827365938349658
30	0.05896419563009795
31	0.006551577292233105
32	0.01637894323058276
33	0.062239984276214494
34	0.04258525239951518
35	0.029482097815048975
36	0.06879156156844761
37	0.07206735021456416
38	0.09499787073738003
39	0.1179283912601959
40	0.06879156156844761
41	0.10154944802961312
42	0.08844629344514691
43	0.08189471615291381
44	0.10810102532184623
45	0.09499787073738003
46	0.10482523667572968
47	0.14741048907524487
48	0.14085891178301177
49	0.14413470042912832
50	0.16378943230582763
51	0.16051364365971107
52	0.15723785501359452
53	0.15396206636747797
54	0.18999574147476006
55	0.21620205064369247
56	0.24895993710485798
57	0.24568414845874145
58	0.2293052052281587
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	30527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.4312903331477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.32486682621786	27.293870999443115
2	13.911835096116729	11.805942280604055
3	7.3650891685323865	9.375307105185573
4	4.632131552536093	7.8618927506797265
5	2.794719370030109	5.92917744947096
6	1.8296919632517565	4.6581714547777375
7	1.235235080676291	3.668883283650539
8	0.818343240948043	2.777868771906837
9	0.6562186366092797	2.505978314279163
>10	2.2620242414884584	16.18239591181577
>50	0.10808306955917549	3.1513086775641233
>100	0.061761754033814564	4.7892030006224
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	343	1.1235955056179776	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.8287745274674877	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	188	0.6158482654699119	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	177	0.5798145903626298	No Hit
TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGATTG	150	0.4913682969174829	No Hit
TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGGCT	136	0.44550725587185114	No Hit
TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCTGC	111	0.36361253971893737	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	104	0.34068201919612146	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83	0.27189045762767383	No Hit
TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.2686146689815573	No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	79	0.2587873030432077	No Hit
TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATG	75	0.24568414845874145	No Hit
TTCCGCGGGACTTCATTTTCTTTGGAAGAAAATGATGTCTAAAGAACATATAGAGGCA	74	0.2424083598126249	No Hit
TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72	0.2358567825203918	No Hit
TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	72	0.2358567825203918	No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70	0.2293052052281587	No Hit
TTCCGCGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA	70	0.2293052052281587	No Hit
TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA	62	0.20309889605922624	No Hit
TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	59	0.1932715301208766	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	58	0.18999574147476006	No Hit
TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTTAC	55	0.18016837553641038	No Hit
TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTGCA	51	0.16706522095194418	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	49	0.16051364365971107	No Hit
TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47	0.15396206636747797	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	46	0.1506862777213614	No Hit
TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTT	46	0.1506862777213614	No Hit
TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCTGT	46	0.1506862777213614	No Hit
TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.14741048907524487	No Hit
TTCCGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGAT	44	0.14413470042912832	No Hit
TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCACCA	42	0.13758312313689522	No Hit
TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	42	0.13758312313689522	No Hit
TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTTTC	41	0.13430733449077864	No Hit
TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTAT	39	0.12775575719854554	No Hit
TTCCGCGGGGACTTCTCTATTACAAAGATAAGAAACGGTCCCACAAATAAAAAAATAA	35	0.11465260261407935	No Hit
TTCCGCGGGGGTGCTATGTGGGGACATTGAGACAAACCAATCTTTTGTACTTCCACTT	34	0.11137681396796278	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAA	34	0.11137681396796278	No Hit
TTCCGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	33	0.10810102532184623	No Hit
TTCCGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	33	0.10810102532184623	No Hit
TTCCGCGGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTTTTCAG	33	0.10810102532184623	No Hit
TTCCGCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	33	0.10810102532184623	No Hit
TTCCGCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCC	32	0.10482523667572968	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA	32	0.10482523667572968	No Hit
TTCCGCGGGAGTGACAAATTATATGTTATAGAAGGTCCAATGTGGGGATAGTGTGTGG	32	0.10482523667572968	No Hit
TTCCGCGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.10154944802961312	No Hit
TTCCGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACACTGACTCCACTTCCAG	31	0.10154944802961312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0032757886461165526	0.0	0.0	0.0	0.0
11	0.009827365938349658	0.0	0.0	0.0	0.0
12	0.009827365938349658	0.0	0.0	0.0	0.0
13	0.009827365938349658	0.0	0.0	0.0	0.0
14	0.019654731876699316	0.0	0.0	0.0	0.0
15	0.022930520522815866	0.0	0.0	0.0	0.0
16	0.02620630916893242	0.0	0.0	0.0	0.0
17	0.02620630916893242	0.0	0.0	0.0	0.0
18	0.02620630916893242	0.0	0.0	0.0	0.0
19	0.02620630916893242	0.0	0.0	0.0	0.0
20	0.03603367510728208	0.0	0.0	0.0	0.0
21	0.03930946375339863	0.0	0.0	0.0	0.0
22	0.03930946375339863	0.0	0.0	0.0	0.0
23	0.03930946375339863	0.0	0.0	0.0	0.0
24	0.04586104104563173	0.0	0.0	0.0	0.0
25	0.04913682969174829	0.0	0.0	0.0	0.0
26	0.04913682969174829	0.0	0.0	0.0	0.0
27	0.04913682969174829	0.0	0.0	0.0	0.0
28	0.08189471615291381	0.0	0.0	0.0	0.0
29	0.08517050479903036	0.0	0.0	0.0	0.0
30	0.09827365938349658	0.0	0.0	0.0	0.0
31	0.10154944802961313	0.0	0.0	0.0	0.0
32	0.10154944802961313	0.0	0.0	0.0	0.0
33	0.10482523667572968	0.0	0.0	0.0	0.0
34	0.10810102532184623	0.0	0.0	0.0	0.0
35	0.1310315458446621	0.0	0.0	0.0	0.0
36	0.1310315458446621	0.0	0.0	0.0	0.0
37	0.1310315458446621	0.0	0.0	0.0	0.0
38	0.1310315458446621	0.0	0.0	0.0	0.0
39	0.1310315458446621	0.0	0.0	0.0	0.0
40	0.13758312313689522	0.0	0.0	0.0	0.0
41	0.14085891178301177	0.0	0.0	0.0	0.0
42	0.14413470042912832	0.0	0.0	0.0	0.0
43	0.14741048907524487	0.0	0.0	0.0	0.0
44	0.14741048907524487	0.0	0.0	0.0	0.0
45	0.14741048907524487	0.0	0.0	0.0	0.0
46	0.14741048907524487	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAAACC	25	1.5989297E-5	52.098846	43
ATTGGCT	20	3.3627253E-4	52.098846	25
TTCTGCC	25	1.5989297E-5	52.098846	29
GCTGTTG	20	3.3627253E-4	52.098846	49
TATTTGC	20	3.3627253E-4	52.098846	29
TACCATG	20	3.3627253E-4	52.098846	31
AGTTCTG	25	1.5989297E-5	52.098846	27
CAAACCT	25	1.5989297E-5	52.098846	44
TTTGCAA	20	3.3627253E-4	52.098846	31
GTTGCCC	20	3.3627253E-4	52.098846	52
AGCCCTC	20	3.3627253E-4	52.098846	41
TGAGACT	15	0.0070994375	52.098843	35
ACACGAG	15	0.0070994375	52.098843	38
ACATGCA	15	0.0070994375	52.098843	45
TGCGCCA	15	0.0070994375	52.098843	44
CCTGCGC	15	0.0070994375	52.098843	42
CACAAGA	15	0.0070994375	52.098843	37
CACCGAC	15	0.0070994375	52.098843	40
AAATACC	15	0.0070994375	52.098843	39
CACGAGT	15	0.0070994375	52.098843	39
>>END_MODULE