SRR212869_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212869_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31809
Sequences flagged as poor quality	0
Sequence length	58
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	355	1.11603634191581	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	232	0.7293533276745575	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	162	0.5092898236348203	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	142	0.44641453676632403	No Hit
TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGATTG	125	0.3929705429281021	No Hit
TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGGCT	116	0.36467666383727876	No Hit
TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCTGC	93	0.29237008393850794	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	92	0.28922631959508316	No Hit
TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84	0.2640762048476846	No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	80	0.25150114747398533	No Hit
TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATG	71	0.22320726838316202	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69	0.21691973969631237	No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68	0.21377597535288756	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	67	0.21063221100946272	No Hit
TTCCGCGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA	64	0.20120091797918827	No Hit
TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.19491338929233865	No Hit
TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTTAC	61	0.19176962494891384	No Hit
TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.188625860605489	No Hit
TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	59	0.1854820962620642	No Hit
TTCCGCGGGACTTCATTTTCTTTGGAAGAAAATGATGTCTAAAGAACATATAGAGGCA	59	0.1854820962620642	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	59	0.1854820962620642	No Hit
TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA	58	0.18233833191863938	No Hit
TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTT	55	0.17290703888836492	No Hit
TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCTGT	50	0.15718821717124085	No Hit
TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTTTC	49	0.15404445282781604	No Hit
TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	46	0.14461315979754158	No Hit
TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTGCA	44	0.13832563111069193	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	41	0.1288943380804175	No Hit
TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	41	0.1288943380804175	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAA	38	0.11946304505014303	No Hit
TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37	0.11631928070671821	No Hit
TTCCGCGGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTTTTCAG	37	0.11631928070671821	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	37	0.11631928070671821	No Hit
TTCCGCGGGAATTTGGGTTCCTTAGCCATTCCCAGAGAGGGGGAGGTGAGGCCCAGAA	37	0.11631928070671821	No Hit
TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTAT	35	0.11003175201986859	No Hit
TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCACCA	35	0.11003175201986859	No Hit
TTCCGCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34	0.10688798767644378	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTTT	15	0.0070865685	52.127964	39
GCTAGCT	15	0.0070865685	52.127964	39
CAATCTT	15	0.0070865685	52.127964	38
CCGCACG	20	3.3556737E-4	52.12796	39
TCTAAAG	20	3.3556737E-4	52.12796	38
CTAAAGA	20	3.3556737E-4	52.12796	39
ACAAGAT	20	3.3556737E-4	52.12796	38
GCCGCAC	25	1.5952268E-5	52.12796	38
GCGGGAT	310	0.0	52.045746	5
GCGGGGT	355	0.0	52.045742	5
GGGTCTT	15	0.0071310448	52.045742	8
GTGTCTA	20	3.382007E-4	52.045742	17
GGGTTTT	15	0.0071310448	52.045742	8
GGCACAT	15	0.0071310448	52.045742	16
AAACATA	20	3.382007E-4	52.045742	15
TTGTAGC	15	0.0071310448	52.045742	23
CGGGGTC	115	0.0	52.045742	6
CGGGGTA	15	0.0071310448	52.045742	6
CGGGGGT	110	0.0	52.045742	6
CGGGGCA	60	0.0	52.045742	6

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content