##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212869_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31809 Sequences flagged as poor quality 0 Sequence length 58 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.502342104435854 39.0 37.0 39.0 33.0 40.0 2 37.229651985287184 39.0 38.0 39.0 33.0 40.0 3 36.93998553868402 39.0 36.0 39.0 33.0 40.0 4 37.293973403753654 39.0 38.0 39.0 33.0 40.0 5 36.15112075198843 38.0 35.0 39.0 30.0 40.0 6 36.164167374013644 38.0 35.0 39.0 30.0 40.0 7 36.821779999371245 38.0 36.0 39.0 33.0 40.0 8 37.16652519727121 39.0 38.0 39.0 33.0 40.0 9 37.15222106950863 39.0 38.0 39.0 33.0 40.0 10 34.763997610739096 38.0 33.0 39.0 27.0 40.0 11 35.21402747650036 38.0 35.0 39.0 28.0 40.0 12 35.745040711748246 38.0 35.0 39.0 29.0 40.0 13 35.76824169260272 38.0 35.0 39.0 29.0 40.0 14 35.75453488006539 38.0 35.0 39.0 29.0 40.0 15 35.84498098022572 38.0 35.0 39.0 29.0 40.0 16 35.483888207739945 38.0 35.0 39.0 28.0 40.0 17 34.726178125687696 38.0 33.0 39.0 27.0 40.0 18 35.12210380709862 38.0 33.0 39.0 28.0 40.0 19 35.039831494231194 38.0 33.0 39.0 28.0 40.0 20 34.91332641705178 38.0 33.0 39.0 27.0 40.0 21 34.91703605897702 38.0 33.0 39.0 27.0 40.0 22 34.84070546071867 38.0 33.0 39.0 27.0 40.0 23 34.63331132698293 38.0 33.0 39.0 27.0 40.0 24 34.608507026313305 38.0 33.0 39.0 27.0 40.0 25 34.57807538746896 37.0 33.0 39.0 27.0 40.0 26 34.170329152126754 37.0 33.0 39.0 25.0 40.0 27 33.92065138797196 37.0 33.0 39.0 25.0 40.0 28 33.719639095853374 36.0 32.0 39.0 24.0 40.0 29 33.36389072275142 36.0 32.0 39.0 23.0 40.0 30 33.473419472476344 36.0 32.0 39.0 23.0 40.0 31 33.07020025778868 36.0 31.0 38.0 22.0 40.0 32 32.846112735389354 36.0 31.0 38.0 22.0 40.0 33 32.74736709736238 36.0 31.0 38.0 22.0 40.0 34 32.38706026596246 36.0 30.0 38.0 20.0 40.0 35 31.924172404036593 35.0 30.0 38.0 18.0 40.0 36 33.29598541293345 37.0 32.0 39.0 22.0 40.0 37 33.212046904964005 36.0 32.0 39.0 22.0 40.0 38 33.06705649344525 36.0 32.0 39.0 22.0 40.0 39 32.83372630387626 36.0 31.0 39.0 21.0 40.0 40 32.89069131377912 36.0 31.0 39.0 21.0 40.0 41 33.15363576346317 37.0 33.0 39.0 22.0 40.0 42 33.0114747398535 37.0 32.0 39.0 21.0 40.0 43 32.85746172466912 36.0 32.0 39.0 20.0 40.0 44 32.41393945109875 36.0 31.0 39.0 17.0 40.0 45 32.553868402024584 36.0 32.0 39.0 19.0 40.0 46 32.09440724323305 36.0 31.0 39.0 13.0 40.0 47 32.036813480461504 36.0 31.0 39.0 15.0 40.0 48 31.90483825332453 36.0 30.0 39.0 15.0 40.0 49 31.84821905749945 36.0 30.0 39.0 14.0 40.0 50 31.429689710459304 36.0 30.0 39.0 7.0 40.0 51 31.26316451318809 36.0 30.0 39.0 2.0 40.0 52 31.189348926404477 36.0 30.0 39.0 2.0 40.0 53 31.00279795026565 36.0 30.0 39.0 2.0 40.0 54 30.827155836398504 36.0 30.0 39.0 2.0 40.0 55 30.675312018611084 36.0 30.0 39.0 2.0 40.0 56 30.20884026533371 36.0 30.0 39.0 2.0 40.0 57 30.06454148197051 35.0 29.0 39.0 2.0 39.0 58 29.853657769813577 35.0 29.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.09071221375206306 2 2 1.0460681860986227 2 3 0.3820420804406126 2 4 1.04695102453816 2 5 0.8690998494497393 2 6 0.9050157955011002 2 7 -4.333549675206584 2 8 -0.4931676143568282 2 9 -0.6352883973832064 2 10 -0.4779097928797853 2 11 -13.538687437408818 2 12 -3.6845340428464652 2 13 -3.5045630883227616 2 14 -3.0648454290681144 2 15 1.361735886926688 2 16 -14.410004710062669 2 17 2.048694423182713 2 18 -13.040840495262454 2 19 -9.69175288649327 2 20 -11.85806622461271 2 21 -12.864335421878643 2 22 -8.384273239740786 2 23 -3.271670392769071 2 24 -11.429867096137933 2 25 -17.533952278357592 2 26 0.03180766839553684 2 27 -4.975396112475586 2 28 -4.100430039318045 2 29 -3.0666754481243963 2 30 -5.92788301739677 2 31 -5.637180620001178 2 32 -11.31514208601456 2 33 -10.882272460810434 2 34 -1.2599406374757471 2 35 -4.314497336390044 2 36 -4.01166744060631 2 37 -1.0449845651546354 2 38 -3.8080558653748255 2 39 -7.420849764339703 2 40 -3.7207276557716185 2 41 -5.777044414922038 2 42 2.278451420408384 2 43 -0.6300939509333574 2 44 4.396878639330957 2 45 4.772063874335647 2 46 -10.781401636239337 2 47 -0.673770971794859 2 48 -1.7939542597976654 2 49 1.429010185684966 2 50 1.7670146527589665 2 51 -9.595938165251347 2 52 1.58232494861236 2 53 -7.0544699205801535 2 54 -2.6607898535734478 2 55 2.1579292433644284 2 56 3.068774977820347 2 57 4.506308473258688 2 58 0.6341061838996005 4 1 -6.909287786247937 4 2 -16.953931813901377 4 3 -21.617957919559387 4 4 -9.95304897546184 4 5 -11.13090015055026 4 6 -16.0949842044989 4 7 -22.333549675206584 4 8 -19.493167614356828 4 9 -23.635288397383206 4 10 -9.477909792879785 4 11 -7.538687437408818 4 12 2.3154659571535348 4 13 -10.504563088322762 4 14 -10.064845429068114 4 15 -10.638264113073312 4 16 -6.410004710062669 4 17 -4.951305576817287 4 18 -0.04084049526245437 4 19 -5.691752886493269 4 20 0.14193377538729024 4 21 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-26.808055865374826 114 39 -26.420849764339703 114 40 -26.72072765577162 114 41 -26.777044414922038 114 42 -26.721548579591616 114 43 -26.630093950933357 114 44 -26.603121360669043 114 45 -26.227936125664353 114 46 -25.781401636239337 114 47 -25.67377097179486 114 48 -25.793954259797665 114 49 -25.570989814315034 114 50 -25.232985347241033 114 51 -24.595938165251347 114 52 -24.41767505138764 114 53 -24.054469920580154 114 54 -23.660789853573448 114 55 -23.84207075663557 114 56 -23.931225022179653 114 57 -23.493691526741312 114 58 -23.3658938161004 115 1 -1.909287786247937 115 2 -0.9539318139013773 115 3 -4.617957919559387 115 4 -0.9530489754618401 115 5 -4.130900150550261 115 6 -0.09498420449889977 115 7 -2.3335496752065836 115 8 0.5068323856431718 115 9 0.3647116026167936 115 10 -0.4779097928797853 115 11 -5.538687437408818 115 12 -0.6845340428464652 115 13 1.4954369116772384 115 14 -3.0648454290681144 115 15 -4.638264113073312 115 16 -8.410004710062669 115 17 0.04869442318271311 115 18 -0.04084049526245437 115 19 0.30824711350673084 115 20 -16.85806622461271 115 21 -6.864335421878643 115 22 -5.384273239740786 115 23 -5.271670392769071 115 24 -12.429867096137933 115 25 1.4660477216424077 115 26 -16.968192331604463 115 27 -3.975396112475586 115 28 -7.100430039318045 115 29 -7.066675448124396 115 30 -1.9278830173967698 115 31 -13.637180620001178 115 32 -8.31514208601456 115 33 -15.882272460810434 115 34 -12.259940637475747 115 35 -12.314497336390044 115 36 -13.01166744060631 115 37 -10.044984565154635 115 38 -15.808055865374826 115 39 -12.420849764339703 115 40 -12.720727655771618 115 41 -1.7770444149220381 115 42 7.278451420408384 115 43 7.369906049066643 115 44 4.396878639330957 115 45 1.7720638743356467 115 46 4.2185983637606626 115 47 4.326229028205141 115 48 2.2060457402023346 115 49 -0.570989814315034 115 50 4.7670146527589665 115 51 -0.5959381652513471 115 52 -0.41767505138763994 115 53 2.9455300794198465 115 54 0.33921014642655223 115 55 4.157929243364428 115 56 10.068774977820347 115 57 1.506308473258688 115 58 3.6341061838996005 116 1 -32.90928778624794 116 2 -31.953931813901377 116 3 -30.617957919559387 116 4 -31.95304897546184 116 5 -30.13090015055026 116 6 -30.0949842044989 116 7 -30.333549675206584 116 8 -31.493167614356828 116 9 -31.635288397383206 116 10 -28.477909792879785 116 11 -29.538687437408818 116 12 -30.684534042846465 116 13 -30.50456308832276 116 14 -30.064845429068114 116 15 -30.638264113073312 116 16 -29.41000471006267 116 17 -28.951305576817287 116 18 -29.040840495262454 116 19 -28.69175288649327 116 20 -28.85806622461271 116 21 -28.864335421878643 116 22 -28.384273239740786 116 23 -28.27167039276907 116 24 -28.429867096137933 116 25 -28.533952278357592 116 26 -27.968192331604463 116 27 -27.975396112475586 116 28 -27.100430039318045 116 29 -27.066675448124396 116 30 -26.92788301739677 116 31 -26.63718062000118 116 32 -26.31514208601456 116 33 -25.882272460810434 116 34 -26.259940637475747 116 35 -25.314497336390044 116 36 -27.01166744060631 116 37 -27.044984565154635 116 38 -26.808055865374826 116 39 -26.420849764339703 116 40 -26.72072765577162 116 41 -26.777044414922038 116 42 -26.721548579591616 116 43 -26.630093950933357 116 44 -26.603121360669043 116 45 -26.227936125664353 116 46 -25.781401636239337 116 47 -25.67377097179486 116 48 -25.793954259797665 116 49 -25.570989814315034 116 50 -25.232985347241033 116 51 -24.595938165251347 116 52 -24.41767505138764 116 53 -24.054469920580154 116 54 -23.660789853573448 116 55 -23.84207075663557 116 56 -23.931225022179653 116 57 -23.493691526741312 116 58 -23.3658938161004 117 1 2.090712213752063 117 2 3.0460681860986227 117 3 3.3820420804406126 117 4 3.04695102453816 117 5 3.8690998494497393 117 6 0.9050157955011002 117 7 3.6664503247934164 117 8 3.506832385643172 117 9 3.3647116026167936 117 10 -3.4779097928797853 117 11 4.461312562591182 117 12 3.3154659571535348 117 13 3.4954369116772384 117 14 -0.06484542906811441 117 15 4.361735886926688 117 16 7.589995289937331 117 17 8.048694423182713 117 18 1.9591595047375456 117 19 -1.6917528864932692 117 20 8.14193377538729 117 21 8.135664578121357 117 22 8.615726760259214 117 23 8.728329607230929 117 24 8.570132903862067 117 25 8.466047721642408 117 26 9.031807668395537 117 27 9.024603887524414 117 28 9.899569960681955 117 29 9.933324551875604 117 30 10.07211698260323 117 31 10.362819379998822 117 32 10.68485791398544 117 33 11.117727539189566 117 34 10.740059362524253 117 35 11.685502663609956 117 36 9.98833255939369 117 37 9.955015434845365 117 38 10.191944134625174 117 39 10.579150235660297 117 40 10.279272344228382 117 41 10.222955585077962 117 42 10.278451420408384 117 43 9.369906049066643 117 44 10.396878639330957 117 45 10.772063874335647 117 46 11.218598363760663 117 47 11.326229028205141 117 48 11.206045740202335 117 49 11.429010185684966 117 50 11.767014652758967 117 51 12.404061834748653 117 52 12.58232494861236 117 53 12.945530079419846 117 54 13.339210146426552 117 55 13.157929243364428 117 56 13.068774977820347 117 57 13.506308473258688 117 58 13.6341061838996 118 1 -5.909287786247937 118 2 -14.953931813901377 118 3 -21.617957919559387 118 4 -17.95304897546184 118 5 -15.13090015055026 118 6 -15.0949842044989 118 7 -22.333549675206584 118 8 -23.493167614356828 118 9 -23.635288397383206 118 10 -18.477909792879785 118 11 -2.538687437408818 118 12 2.3154659571535348 118 13 -8.504563088322762 118 14 -8.064845429068114 118 15 -1.638264113073312 118 16 -1.410004710062669 118 17 -12.951305576817287 118 18 -5.040840495262454 118 19 -21.69175288649327 118 20 -0.8580662246127098 118 21 7.135664578121357 118 22 7.615726760259214 118 23 7.728329607230929 118 24 7.570132903862067 118 25 7.466047721642408 118 26 5.031807668395537 118 27 5.024603887524414 118 28 2.899569960681955 118 29 4.933324551875604 118 30 -11.92788301739677 118 31 -8.637180620001178 118 32 -5.315142086014561 118 33 -17.882272460810434 118 34 -9.259940637475747 118 35 -8.314497336390044 118 36 -12.01166744060631 118 37 -7.0449845651546354 118 38 -10.808055865374826 118 39 -11.420849764339703 118 40 -7.7207276557716185 118 41 -9.777044414922038 118 42 -12.721548579591616 118 43 -3.6300939509333574 118 44 -3.603121360669043 118 45 3.7720638743356467 118 46 -25.781401636239337 118 47 -25.67377097179486 118 48 -25.793954259797665 118 49 -25.570989814315034 118 50 -25.232985347241033 118 51 -24.595938165251347 118 52 -24.41767505138764 118 53 -24.054469920580154 118 54 -23.660789853573448 118 55 -23.84207075663557 118 56 -23.931225022179653 118 57 -23.493691526741312 118 58 -23.3658938161004 119 1 -32.90928778624794 119 2 -31.953931813901377 119 3 -30.617957919559387 119 4 -31.95304897546184 119 5 -30.13090015055026 119 6 -30.0949842044989 119 7 -30.333549675206584 119 8 -31.493167614356828 119 9 -31.635288397383206 119 10 -28.477909792879785 119 11 -29.538687437408818 119 12 -30.684534042846465 119 13 -30.50456308832276 119 14 -30.064845429068114 119 15 -30.638264113073312 119 16 -29.41000471006267 119 17 -28.951305576817287 119 18 -29.040840495262454 119 19 -28.69175288649327 119 20 -28.85806622461271 119 21 -28.864335421878643 119 22 -28.384273239740786 119 23 -28.27167039276907 119 24 -28.429867096137933 119 25 -28.533952278357592 119 26 -27.968192331604463 119 27 -27.975396112475586 119 28 -27.100430039318045 119 29 -27.066675448124396 119 30 -26.92788301739677 119 31 -26.63718062000118 119 32 -26.31514208601456 119 33 -25.882272460810434 119 34 -26.259940637475747 119 35 -25.314497336390044 119 36 -27.01166744060631 119 37 -27.044984565154635 119 38 -26.808055865374826 119 39 -26.420849764339703 119 40 -26.72072765577162 119 41 -26.777044414922038 119 42 -26.721548579591616 119 43 -26.630093950933357 119 44 -26.603121360669043 119 45 -26.227936125664353 119 46 -25.781401636239337 119 47 -25.67377097179486 119 48 -25.793954259797665 119 49 -25.570989814315034 119 50 -25.232985347241033 119 51 -24.595938165251347 119 52 -24.41767505138764 119 53 -24.054469920580154 119 54 -23.660789853573448 119 55 -23.84207075663557 119 56 -23.931225022179653 119 57 -23.493691526741312 119 58 -23.3658938161004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116.0 3 11.0 4 16.0 5 15.0 6 29.0 7 38.0 8 34.0 9 65.0 10 71.0 11 77.0 12 82.0 13 109.0 14 120.0 15 112.0 16 158.0 17 148.0 18 152.0 19 180.0 20 206.0 21 208.0 22 255.0 23 271.0 24 354.0 25 405.0 26 529.0 27 628.0 28 720.0 29 814.0 30 1064.0 31 1236.0 32 1432.0 33 1818.0 34 2076.0 35 2566.0 36 3349.0 37 4419.0 38 4444.0 39 3461.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 55.39277595352362 32.79552917403385 3.0468552664814346 8.764839605961102 11 30.84106272598648 24.81370853639365 25.769533092281087 18.575695645338783 12 30.434782608695656 16.630513376717282 27.36018108082618 25.574522933760885 13 22.559652928416483 19.029205570750417 30.31531956364551 28.095821937187587 14 20.13581061963595 25.91719324719419 32.40906661636644 21.53792951680342 15 25.64054198497281 28.966644660316266 26.19070074507215 19.20211260963878 16 31.10126064950171 28.14926593102581 23.01235499386966 17.737118425602816 17 29.78355250739319 28.56918140061662 21.16655131189832 20.480714780091862 18 28.010940299915116 27.709138922946337 23.29215002043447 20.987770756704077 19 25.420478480933067 31.211292401521582 23.584520104372977 19.783709013172373 20 26.43277059951586 28.85661290829639 24.58738092992549 20.123235562262252 21 25.194127448206483 27.20613662799837 26.96721053789808 20.632525385897075 22 22.24283782508884 26.453662064844806 28.724173716154596 22.579326393911757 23 24.530793171743845 29.551384828193278 25.958062183658715 19.959759816404162 24 23.870602659624634 30.74601527869471 24.307585903360685 21.075796158319974 25 23.273070271969818 32.59864801131898 20.16664046533564 23.96164125137557 26 23.92200031451486 32.29438590973424 22.81176285579494 20.971850919955966 27 19.674812089190805 29.660030820517658 21.788219014372427 28.876938075919114 28 21.795073457702834 30.537641174064866 24.034982854626104 23.63230251360619 29 26.35196922397755 27.490303660959224 23.387885094440765 22.76984202062246 30 22.62133173969113 29.056710596672225 25.716352656245085 22.605605007391564 31 28.503600289280882 27.52884947960884 22.567053422633084 21.400496808477186 32 27.528619952195243 29.236381934834572 24.75468612404076 18.480311988929422 33 27.90324609964771 27.15148465022647 25.553598389531956 19.39167086059386 34 24.400062902972167 29.64617078156943 26.05126592231483 19.902500393143576 35 22.979901236121158 33.59859088478596 24.68782436385368 18.733683515239203 36 23.27279594598848 33.83274054955777 23.766957288092915 19.127506216360832 37 26.973435729573207 32.619916907969284 22.085484074027445 18.321163288430064 38 24.384562110432537 33.66807278221998 22.332053138575837 19.615311968771643 39 25.289636065986652 32.0299710363934 21.152877471351214 21.52751542626873 40 23.612728588964778 31.30842592301155 22.473324730099776 22.60552075792389 41 21.424748953316332 34.55157868228035 23.197028362766392 20.826644001636918 42 21.328506217535022 31.42137572800252 23.49126396977806 23.7588540846844 43 21.554302988317538 30.69559467204081 23.717605567276507 24.032496772365146 44 22.84957259565341 27.754471185692204 24.77052644860108 24.625429770053305 45 24.80398022483232 30.35551217054508 22.64382655792424 22.196681046698366 46 22.362537003212193 31.026012470869812 23.795427347735718 22.816023178182277 47 21.92579060098274 30.776741842005794 24.86770820209147 22.429759354919995 48 22.94947713241779 28.864810381756328 27.05052286758221 21.13518961824367 49 25.316575316575317 28.980028980028983 24.595224595224597 21.10817110817111 50 20.260182064447037 29.404353167228397 25.564620279081492 24.77084448924308 51 20.73278306344906 29.979207359334637 28.057463297838826 21.230546279377478 52 21.909262759924385 29.20919974795211 25.771896660365467 23.109640831758036 53 20.75602457079855 30.06142699637738 27.361789258150893 21.820759174673178 54 20.23186944741982 30.366706571734607 27.959800894713627 21.44162308613194 55 20.87783974540757 29.804329331694866 24.665217254308853 24.652613668588714 56 21.59040706847586 31.079204796465763 23.950773114547175 23.379615020511203 57 22.091264338837764 33.303920332787094 22.882263960670617 21.722551367704526 58 22.74618585298197 35.20993569537259 21.847812381792963 20.196066069852478 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 46.0 12 92.0 13 343.5 14 595.0 15 713.5 16 672.5 17 513.0 18 395.0 19 277.0 20 238.5 21 200.0 22 158.5 23 123.5 24 130.0 25 127.0 26 124.0 27 166.0 28 236.5 29 265.0 30 308.5 31 352.0 32 373.0 33 394.0 34 423.0 35 578.0 36 704.0 37 647.5 38 591.0 39 698.5 40 806.0 41 771.0 42 729.5 43 723.0 44 875.0 45 1027.0 46 1056.0 47 1160.0 48 1235.0 49 1184.0 50 1133.0 51 1215.0 52 1297.0 53 1350.0 54 1539.5 55 1676.0 56 1850.5 57 2025.0 58 1996.5 59 2067.0 60 2166.0 61 2313.0 62 2460.0 63 2198.0 64 1936.0 65 1670.0 66 1196.0 67 988.0 68 830.5 69 673.0 70 546.5 71 420.0 72 366.0 73 276.0 74 240.0 75 173.5 76 107.0 77 88.0 78 54.0 79 39.0 80 30.0 81 21.0 82 14.5 83 8.0 84 6.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.4306957150491999 11 0.012575057373699267 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.07230657989877079 18 0.0 19 0.0 20 0.0 21 0.0 22 0.031437643434248166 23 0.0 24 0.0 25 0.012575057373699267 26 0.04401270080794744 27 0.03772517212109781 28 0.06916281555534597 29 0.30180137696878245 30 0.050300229494797066 31 0.018862586060548903 32 0.040868936464522616 33 0.05344399383822189 34 0.04401270080794744 35 0.050300229494797066 36 0.11946304505014303 37 0.11631928070671821 38 0.13518186676726712 39 0.14146939545411674 40 0.11946304505014303 41 0.1320381024238423 42 0.13832563111069193 43 0.16347574585809047 44 0.3332390204030306 45 0.16347574585809047 46 0.17290703888836492 47 0.19176962494891384 48 0.19176962494891384 49 0.19805715363576346 50 0.19491338929233865 51 0.21063221100946272 52 0.21691973969631237 53 0.20120091797918827 54 0.21063221100946272 55 0.22635103272658683 56 0.3741079568675532 57 0.24206985444371087 58 0.26721996919110946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 31809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.36681442359081 #Duplication Level Percentage of deduplicated Percentage of total 1 70.97172570685733 34.32676286585558 2 11.998700032499187 11.606777955924425 3 5.862853428664283 8.507026313307554 4 3.5489112772180698 6.865981326039799 5 2.1319467013324664 5.155773523216699 6 1.4819629509262269 4.30066962180515 7 0.9359766005849854 3.1689144581722153 8 0.6369840753981151 2.4647112452450566 9 0.3834904127396815 1.6693388663585778 >10 1.8979525511862203 14.725392184601843 >50 0.11049723756906078 3.6499104027162126 >100 0.03899902502437439 3.5587412367568927 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 355 1.11603634191581 No Hit TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 232 0.7293533276745575 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 162 0.5092898236348203 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 142 0.44641453676632403 No Hit TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGATTG 125 0.3929705429281021 No Hit TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGGCT 116 0.36467666383727876 No Hit TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCTGC 93 0.29237008393850794 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 92 0.28922631959508316 No Hit TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.2640762048476846 No Hit TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 80 0.25150114747398533 No Hit TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATG 71 0.22320726838316202 No Hit TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.21691973969631237 No Hit TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.21377597535288756 No Hit TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 67 0.21063221100946272 No Hit TTCCGCGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA 64 0.20120091797918827 No Hit TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62 0.19491338929233865 No Hit TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTTAC 61 0.19176962494891384 No Hit TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.188625860605489 No Hit TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 59 0.1854820962620642 No Hit TTCCGCGGGACTTCATTTTCTTTGGAAGAAAATGATGTCTAAAGAACATATAGAGGCA 59 0.1854820962620642 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 59 0.1854820962620642 No Hit TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTACA 58 0.18233833191863938 No Hit TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTT 55 0.17290703888836492 No Hit TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCTGT 50 0.15718821717124085 No Hit TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTTTC 49 0.15404445282781604 No Hit TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 46 0.14461315979754158 No Hit TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTGCA 44 0.13832563111069193 No Hit TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 41 0.1288943380804175 No Hit TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 41 0.1288943380804175 No Hit TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAA 38 0.11946304505014303 No Hit TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.11631928070671821 No Hit TTCCGCGGGATGCCAAATGTTAACTGCCAAGCTTGGAGTGACCTAAAGCCTTTTTCAG 37 0.11631928070671821 No Hit TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 37 0.11631928070671821 No Hit TTCCGCGGGAATTTGGGTTCCTTAGCCATTCCCAGAGAGGGGGAGGTGAGGCCCAGAA 37 0.11631928070671821 No Hit TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTTAT 35 0.11003175201986859 No Hit TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCACCA 35 0.11003175201986859 No Hit TTCCGCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.10688798767644378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.00943129303027445 0.0 0.0 0.0 0.0 12 0.00943129303027445 0.0 0.0 0.0 0.0 13 0.00943129303027445 0.0 0.0 0.0 0.0 14 0.022006350403973717 0.0 0.0 0.0 0.0 15 0.022006350403973717 0.0 0.0 0.0 0.0 16 0.022006350403973717 0.0 0.0 0.0 0.0 17 0.022006350403973717 0.0 0.0 0.0 0.0 18 0.022006350403973717 0.0 0.0 0.0 0.0 19 0.022006350403973717 0.0 0.0 0.0 0.0 20 0.028293879090823353 0.0 0.0 0.0 0.0 21 0.031437643434248166 0.0 0.0 0.0 0.0 22 0.031437643434248166 0.0 0.0 0.0 0.0 23 0.03458140777767298 0.0 0.0 0.0 0.0 24 0.040868936464522616 0.0 0.0 0.0 0.0 25 0.040868936464522616 0.0 0.0 0.0 0.0 26 0.04401270080794743 0.0 0.0 0.0 0.0 27 0.05030022949479707 0.0 0.0 0.0 0.0 28 0.06601905121192116 0.0 0.0 0.0 0.0 29 0.06601905121192116 0.0 0.0 0.0 0.0 30 0.06916281555534597 0.0 0.0 0.0 0.0 31 0.06916281555534597 0.0 0.0 0.0 0.0 32 0.06916281555534597 0.0 0.0 0.0 0.0 33 0.07230657989877079 0.0 0.0 0.0 0.0 34 0.0754503442421956 0.0 0.0 0.0 0.0 35 0.08802540161589487 0.0 0.0 0.0 0.0 36 0.09116916595931969 0.0 0.0 0.0 0.0 37 0.09431293030274451 0.0 0.0 0.0 0.0 38 0.09431293030274451 0.0 0.0 0.0 0.0 39 0.09431293030274451 0.0 0.0 0.0 0.0 40 0.09745669464616932 0.0 0.0 0.0 0.0 41 0.10060045898959415 0.0 0.0 0.0 0.0 42 0.10060045898959415 0.0 0.0 0.0 0.0 43 0.10060045898959415 0.0 0.0 0.0 0.0 44 0.10060045898959415 0.0 0.0 0.0 0.0 45 0.10060045898959415 0.0 0.0 0.0 0.0 46 0.10060045898959415 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCTTT 15 0.0070865685 52.127964 39 GCTAGCT 15 0.0070865685 52.127964 39 CAATCTT 15 0.0070865685 52.127964 38 CCGCACG 20 3.3556737E-4 52.12796 39 TCTAAAG 20 3.3556737E-4 52.12796 38 CTAAAGA 20 3.3556737E-4 52.12796 39 ACAAGAT 20 3.3556737E-4 52.12796 38 GCCGCAC 25 1.5952268E-5 52.12796 38 GCGGGAT 310 0.0 52.045746 5 GCGGGGT 355 0.0 52.045742 5 GGGTCTT 15 0.0071310448 52.045742 8 GTGTCTA 20 3.382007E-4 52.045742 17 GGGTTTT 15 0.0071310448 52.045742 8 GGCACAT 15 0.0071310448 52.045742 16 AAACATA 20 3.382007E-4 52.045742 15 TTGTAGC 15 0.0071310448 52.045742 23 CGGGGTC 115 0.0 52.045742 6 CGGGGTA 15 0.0071310448 52.045742 6 CGGGGGT 110 0.0 52.045742 6 CGGGGCA 60 0.0 52.045742 6 >>END_MODULE