SRR212868_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212868_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74031
Sequences flagged as poor quality	0
Sequence length	56
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	936	1.2643352109251529	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	859	1.1603247288298144	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	551	0.7442828004484607	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	505	0.6821466682876093	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	431	0.5821885426375437	No Hit
TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGG	334	0.4511623509070525	No Hit
TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGAT	289	0.39037700422795857	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	251	0.3390471559211682	No Hit
TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAA	238	0.3214869446583188	No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	236	0.31878537369480353	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	219	0.2958220205049236	No Hit
TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCT	212	0.28636652213262015	No Hit
TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	183	0.24719374316164852	No Hit
TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	164	0.22152881900825333	No Hit
TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCT	160	0.21612567708122274	No Hit
TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA	152	0.20531939322716158	No Hit
TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	147	0.1985654658183734	No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	128	0.17290054166497817	No Hit
TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	117	0.1580419013656441	No Hit
TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTG	116	0.15669111588388648	No Hit
TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	111	0.14993718847509827	No Hit
TTCCGCGGGCTTTTGAAATTTTTTTCAGGTGACTTAATGTATCTTAATAACTACTA	107	0.1445340465480677	No Hit
TTCCGCGGGGTTGTATGATGCCACTTTCCATGTGTATGTGATACCACTGTACATGT	100	0.1350785481757642	No Hit
TTCCGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	100	0.1350785481757642	No Hit
TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99	0.13372776269400657	No Hit
TTCCGCGGGAATTTGGGTTCCTTAGCCATTCCCAGAGAGGGGGAGGTGAGGCCCAG	99	0.13372776269400657	No Hit
TTCCGCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	99	0.13372776269400657	No Hit
TTCCGCGGGGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGG	99	0.13372776269400657	No Hit
TTCCGCGGGGTGGAGCTGAGTATGTGGCTGAGGGCCTGAGGCAGGAGCAGGGGGCG	96	0.12967540624873364	No Hit
TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTT	95	0.12832462076697598	No Hit
TTCCGCGGGATGATTAGCTTCTTCTAGCCCAGTGATCAGTCATGGCATGCCCTCTG	93	0.12562304980346073	No Hit
TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTT	91	0.12292147883994542	No Hit
TTCCGCGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATG	88	0.11886912239467251	No Hit
TTCCGCGGGGATTTTCTGGAGGTTGATAACTTTGCTTGCGGTAGATTTTCTTTAAA	88	0.11886912239467251	No Hit
TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA	88	0.11886912239467251	No Hit
TTCCGCGGGACTTCATTTTCTTTGGAAGAAAATGATGTCTAAAGAACATATAGAGG	86	0.11616755143115721	No Hit
TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTT	86	0.11616755143115721	No Hit
TTCCGCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	86	0.11616755143115721	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	83	0.11211519498588429	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTG	81	0.109413624022369	No Hit
TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCT	80	0.10806283854061137	No Hit
TTCCGCGGGATTTTCACTGTTAAAAAAAAATTTCATCTTTTTTGGAATTTTAAAAT	80	0.10806283854061137	No Hit
TTCCGCGGGAGTGACAAATTATATGTTATAGAAGGTCCAATGTGGGGATAGTGTGT	79	0.10671205305885373	No Hit
TTCCGCGGGGATGAAAGGAAAAAGAATGCCAAAATCAGCAGCCTCCTGGAGGAGCA	75	0.10130891113182315	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGAGG	20	4.09771E-4	50.148777	50
AGAAGAC	15	0.00830182	50.148773	49
ACTTCGC	15	0.00830182	50.148773	48
AAAGTCT	15	0.00830182	50.148773	48
GCACAAG	30	1.0134627E-6	50.148773	49
CCGCCCC	15	0.00830182	50.148773	48
CTTAATG	15	0.008324155	50.11473	33
GAAATTG	20	4.1114815E-4	50.11473	47
TACCCAA	15	0.008324155	50.11473	43
TTAATGT	15	0.008324155	50.11473	34
AATCAGC	15	0.008324155	50.11473	33
GTCTAGC	20	4.1114815E-4	50.11473	32
CATTCTA	15	0.008324155	50.11473	33
TACATGC	30	1.0182284E-6	50.11473	44
TGCATGG	20	4.1114815E-4	50.11473	45
TATTGAG	15	0.008324155	50.11473	44
ACTAGGT	15	0.008324155	50.11473	39
TAACCTG	15	0.008324155	50.11473	35
AAGCCAT	20	4.1114815E-4	50.11473	37
GAGCGTG	15	0.008324155	50.11473	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content