SRR212867_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212867_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68911
Sequences flagged as poor quality	0
Sequence length	56
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	908	1.317641595681386	No Hit
TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	770	1.1173832914919244	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	479	0.6950994761358854	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	458	0.6646253863679239	No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	366	0.531119850241616	No Hit
TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGG	308	0.44695331659676973	No Hit
TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	240	0.34827531163384656	No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	238	0.3453730173702312	No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	234	0.33956842884300037	No Hit
TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGAT	234	0.33956842884300037	No Hit
TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAA	211	0.3061920448114234	No Hit
TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCT	172	0.24959730667092334	No Hit
TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	162	0.23508583535284644	No Hit
TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	151	0.2191232169029618	No Hit
TTCCGCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	150	0.21767206977115408	No Hit
TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	147	0.213318628375731	No Hit
TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA	132	0.1915514213986156	No Hit
TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCT	118	0.1712353615533079	No Hit
TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	116	0.1683330672896925	No Hit
TTCCGCGGGAATTTGGGTTCCTTAGCCATTCCCAGAGAGGGGGAGGTGAGGCCCAG	114	0.16543077302607712	No Hit
TTCCGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	113	0.1639796258942694	No Hit
TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	108	0.15672389023523095	No Hit
TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106	0.15382159597161557	No Hit
TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA	105	0.15237044883980788	No Hit
TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTT	103	0.1494681545761925	No Hit
TTCCGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC	103	0.1494681545761925	No Hit
TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	98	0.142212418917154	No Hit
TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCAC	92	0.13350553612630783	No Hit
TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTT	88	0.12770094759907707	No Hit
TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCT	82	0.1189940648082309	No Hit
TTCCGCGGGCTTTTGAAATTTTTTTCAGGTGACTTAATGTATCTTAATAACTACTA	82	0.1189940648082309	No Hit
TTCCGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	82	0.1189940648082309	No Hit
TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81	0.11754291767642322	No Hit
TTCCGCGGGATTTTCACTGTTAAAAAAAAATTTCATCTTTTTTGGAATTTTAAAAT	81	0.11754291767642322	No Hit
TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTT	79	0.11464062341280783	No Hit
TTCCGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	78	0.11318947628100014	No Hit
TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTG	78	0.11318947628100014	No Hit
TTCCGCGGGGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTT	78	0.11318947628100014	No Hit
TTCCGCGGGGTTGTATGATGCCACTTTCCATGTGTATGTGATACCACTGTACATGT	75	0.10883603488557704	No Hit
TTCCGCGGGGTGGAGCTGAGTATGTGGCTGAGGGCCTGAGGCAGGAGCAGGGGGCG	75	0.10883603488557704	No Hit
TTCCGCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	73	0.10593374062196166	No Hit
TTCCGCGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATG	72	0.10448259349015397	No Hit
TTCCGCGGGATTTTTGAAATAAATTTTTTAATTTATAGAGTATTTCTGAGGGGTGG	72	0.10448259349015397	No Hit
TTCCGCGGGGTGGGTCATCAGCCCATGCCTCACCCTGGCAGTGTGCTCTAACTGAG	71	0.10303144635834627	No Hit
TTCCGCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG	69	0.10012915209473087	No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG	69	0.10012915209473087	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGAGG	15	0.00818727	50.321346	50
GCCTGCA	15	0.00818727	50.321346	49
CATGCAC	25	1.998491E-5	50.28446	46
CATATAG	15	0.008211063	50.284454	47
ATATAGA	15	0.008211063	50.284454	48
GTAGTTT	15	0.008211063	50.284454	47
TAACTAC	15	0.008211063	50.284454	48
AAATACC	45	1.2732926E-10	50.21084	39
GTTAAAT	45	1.2732926E-10	50.21084	36
AATACCT	45	1.2732926E-10	50.21084	40
CAGCGTA	15	0.008258805	50.210835	36
AGCGTAG	15	0.008258805	50.210835	37
CATATGG	15	0.008258805	50.210835	40
GTCTGGT	15	0.008258805	50.210835	34
GCGGTAG	15	0.008258805	50.210835	38
TTTGTCG	15	0.008258805	50.210835	37
ATTACAT	20	4.0707015E-4	50.210835	34
TTGTCGG	15	0.008258805	50.210835	38
TGCGGTA	15	0.008258805	50.210835	37
TACATGC	25	2.0159187E-5	50.210835	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content