##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212867_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68911 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.33901699293291 39.0 38.0 40.0 35.0 40.0 2 38.312301374236334 39.0 38.0 40.0 35.0 40.0 3 38.25805749444936 39.0 38.0 40.0 35.0 40.0 4 38.33141298196224 39.0 38.0 40.0 35.0 40.0 5 37.82583332124044 39.0 38.0 40.0 35.0 40.0 6 37.813222852665035 39.0 38.0 40.0 35.0 40.0 7 38.34116469068799 39.0 38.0 40.0 36.0 40.0 8 38.301838603416 39.0 38.0 40.0 36.0 40.0 9 38.30253515403927 39.0 38.0 40.0 36.0 40.0 10 36.92829882021738 39.0 36.0 40.0 31.0 40.0 11 36.70853709857642 39.0 35.0 40.0 31.0 40.0 12 37.13427464410616 39.0 36.0 40.0 32.0 40.0 13 37.234070032360584 39.0 37.0 40.0 33.0 40.0 14 37.19278489646065 39.0 37.0 40.0 33.0 40.0 15 37.18245272888218 39.0 37.0 40.0 33.0 40.0 16 37.085298428407654 39.0 36.0 40.0 33.0 40.0 17 36.90100274266808 38.0 36.0 40.0 33.0 40.0 18 36.794401474365486 38.0 36.0 40.0 32.0 40.0 19 36.69314042750794 38.0 36.0 40.0 32.0 40.0 20 36.62048149061833 38.0 36.0 40.0 31.0 40.0 21 36.49836745947672 38.0 36.0 40.0 31.0 40.0 22 36.45037802382784 38.0 36.0 40.0 31.0 40.0 23 36.1370318236566 38.0 35.0 40.0 30.0 40.0 24 36.066230355095705 38.0 35.0 40.0 30.0 40.0 25 36.04340381071237 38.0 35.0 40.0 30.0 40.0 26 35.55008634325434 38.0 35.0 39.0 29.0 40.0 27 35.43802876173615 38.0 35.0 39.0 29.0 40.0 28 35.05608683664437 38.0 35.0 39.0 28.0 40.0 29 35.042097778293744 38.0 35.0 39.0 28.0 40.0 30 34.610512109822814 38.0 33.0 39.0 27.0 40.0 31 34.112594505956956 38.0 33.0 39.0 26.0 40.0 32 33.76732306888596 38.0 33.0 39.0 23.0 40.0 33 33.68222780107675 38.0 33.0 39.0 23.0 40.0 34 33.89040936860588 38.0 33.0 39.0 24.0 40.0 35 33.60869817590805 38.0 33.0 39.0 23.0 40.0 36 33.61707129485858 38.0 33.0 39.0 23.0 40.0 37 33.40051660837892 38.0 33.0 39.0 22.0 40.0 38 33.1922624834932 37.0 33.0 39.0 21.0 40.0 39 33.2025366051864 38.0 33.0 39.0 21.0 40.0 40 32.90043679528667 37.0 33.0 39.0 18.0 40.0 41 32.28227714007923 37.0 32.0 39.0 10.0 40.0 42 32.1332588411139 36.0 32.0 39.0 9.0 40.0 43 31.98672200374396 36.0 31.0 39.0 5.0 40.0 44 31.71844843348667 36.0 31.0 39.0 2.0 40.0 45 31.427130646776277 36.0 31.0 39.0 2.0 40.0 46 30.787740709030487 36.0 30.0 39.0 2.0 40.0 47 30.494667034290607 35.0 30.0 39.0 2.0 40.0 48 30.232967160540408 35.0 29.0 38.0 2.0 40.0 49 29.953708406495334 35.0 29.0 39.0 2.0 40.0 50 29.620089680892747 35.0 29.0 38.0 2.0 40.0 51 28.898767976085097 35.0 28.0 38.0 2.0 40.0 52 28.746339481360014 35.0 27.0 38.0 2.0 40.0 53 28.425099040791746 35.0 27.0 38.0 2.0 40.0 54 28.059801773301796 35.0 25.0 38.0 2.0 40.0 55 27.696521600325056 34.0 24.0 38.0 2.0 40.0 56 16.03372465934321 20.0 2.0 23.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.23369756602730973 1 2 -0.14048199852339138 1 3 -0.30665441375982994 1 4 -0.7180475724574649 1 5 -0.4868174887587102 1 6 -0.005089619330426842 1 7 -0.14385954502414933 1 8 -0.45295985265507 1 9 0.0326037052058652 1 10 0.1939058910278746 1 11 -0.06598034370223615 1 12 0.35912912487319915 1 13 -0.20254625727329767 1 14 0.425959860348037 1 15 -0.4296563512001583 1 16 0.6465953760349521 1 17 -0.011360007522632998 1 18 0.10354515333774117 1 19 0.37082112232985054 1 20 0.42383999653680604 1 21 0.5978285891423525 1 22 -0.1355558062279414 1 23 0.31888511142875586 1 24 0.20150898835024833 1 25 -0.0887482780356379 1 26 0.6721637151167243 1 27 0.6682296187702477 1 28 1.1031257363011022 1 29 0.4824107267661404 1 30 1.3621528966935017 1 31 1.4330212287804898 1 32 2.0749135556014195 1 33 0.4312925002596586 1 34 1.515001655240539 1 35 1.6992251387707142 1 36 2.042477572725005 1 37 1.6637422323705948 1 38 0.9912058302734508 1 39 1.9995316985486298 1 40 0.9482081803188507 1 41 0.5160485052946591 1 42 1.0380176121029265 1 43 1.5040617091832402 1 44 1.6903639735137581 1 45 1.237748128761357 1 46 1.2256400573223374 1 47 1.57337001705244 1 48 1.4367821210258818 1 49 1.801207588691561 1 50 1.5571277030503445 1 51 0.9144216730234369 1 52 1.4481984755392716 1 53 1.3990662104754144 1 54 0.01631126679264483 1 55 -1.1486168650886377 1 56 -0.3024797120460079 2 1 0.13627762007691047 2 2 -0.03874502581867745 2 3 -0.31757252790375645 2 4 -0.17412697692396506 2 5 0.2389889628541937 2 6 -0.5770499170971775 2 7 -0.3344302646271302 2 8 -0.05196729682380408 2 9 -0.28327718809438807 2 10 -1.253736788872871 2 11 -2.0443922543722124 2 12 -1.6770991629679912 2 13 -1.2918762820871947 2 14 -1.9348341843169763 2 15 -1.4740732246492954 2 16 -0.5648190160246003 2 17 -1.3701689405251116 2 18 -0.6721372287962524 2 19 -0.9864989769257306 2 20 -0.32032873795450456 2 21 -0.9587470932397792 2 22 -0.33481138935449195 2 23 -0.16622655110226248 2 24 -0.8592850563147678 2 25 -0.6438351266708722 2 26 -0.9811861608137917 2 27 -0.16154705616771992 2 28 -0.5911670676691188 2 29 -0.5198225238542022 2 30 -0.8038520660856534 2 31 -1.0667306322616952 2 32 -0.3129276354655772 2 33 -0.9252831821224774 2 34 -0.8966608757768313 2 35 -1.43551431532358 2 36 -1.1371750327340493 2 37 -1.379185807331634 2 38 -1.3777767999995056 2 39 -0.6925278548012486 2 40 -0.9604766832047211 2 41 -0.2688150182785378 2 42 0.38764540366620537 2 43 -0.03812191364553996 2 44 0.8749793581291421 2 45 -0.024038471734918687 2 46 0.353431620597771 2 47 0.8783327962087668 2 48 -0.759743933564689 2 49 0.8294954298826269 2 50 0.523628943745134 2 51 0.40127030825916776 2 52 1.0395136120156963 2 53 1.1759892873984903 2 54 0.5644502246090219 2 55 1.204236732926251 2 56 -0.2632737567110208 3 1 0.26630243397269027 3 2 -0.12435296626532732 3 3 0.16108752172403484 3 4 0.1045330727038234 3 5 0.02931154349935383 3 6 -0.08573478062074713 3 7 0.2271081969113311 3 8 0.36962079250621827 3 9 0.11324886649618549 3 10 -0.22544894768180512 3 11 0.030793849846155297 3 12 0.036548479711910886 3 13 0.44261503304927885 3 14 -0.4933949783616427 3 15 -0.7038498995872615 3 16 0.3078856986155998 3 17 0.5854141860257513 3 18 -0.18677742730741898 3 19 -0.3388562970249893 3 20 0.7625496739561655 3 21 -0.38604237859958346 3 22 -0.780717096550525 3 23 0.28662704691262775 3 24 -0.3146200439078157 3 25 -0.4758450522291895 3 26 0.2689379086651158 3 27 0.2811328445766961 3 28 0.6998999298494866 3 29 0.5953139525725959 3 30 -0.6862342000806905 3 31 -0.45407554541306183 3 32 -0.7315380573018118 3 33 -1.7138687900629215 3 34 -1.1301596350820446 3 35 -1.3169038934873534 3 36 -1.489780491791123 3 37 -1.0459351869842415 3 38 -0.6055683632749407 3 39 -0.7101457208062101 3 40 -0.8098563358101778 3 41 -0.17749988180211318 3 42 -2.0748856137035254 3 43 -1.157228613397404 3 44 -1.69673280067979 3 45 -1.5041873551096074 3 46 -1.7743599426776626 3 47 -1.4750170797217557 3 48 -2.047088846716054 3 49 -2.021373056469727 3 50 -0.5557755227561074 3 51 -1.1178363914926948 3 52 -1.0518015244607284 3 53 -0.8751273379116817 3 54 -0.8546564751428392 3 55 -2.0357136392821857 3 56 -0.4637700346266538 4 1 -0.4831935337692457 4 2 -0.894514256587911 4 3 -0.3192552202114456 4 4 -0.18830966923165704 4 5 -0.6360110371458063 4 6 -1.0746460709433308 4 7 -0.5228918030886689 4 8 -0.5356211429776536 4 9 -1.0198156496328465 4 10 -1.0102271734882535 4 11 0.22988659178163573 4 12 -0.4674837783526087 4 13 -0.04428012824104144 4 14 0.03583889260610107 4 15 0.1756863907353221 4 16 -0.3402997852553682 4 17 -0.5824285559097291 4 18 0.46896854043451697 4 19 -0.16798936154111743 4 20 -0.4914825841083541 4 21 0.07514713752944857 4 22 0.5055732260301227 4 23 -0.014784243409955877 4 24 -0.07923697939168761 4 25 0.8114533348675863 4 26 1.162083069955436 4 27 1.132443328447664 4 28 0.8309483169462624 4 29 0.6371486299919482 4 30 1.5310037031451174 4 31 1.396730906199842 4 32 2.421687749149804 4 33 1.3773610486467547 4 34 2.3300218165308593 4 35 2.7954953000610345 4 36 1.9572961211121012 4 37 2.0357180388222105 4 38 1.611165507692803 4 39 1.463241375967982 4 40 1.6246194706414343 4 41 2.9898388278753067 4 42 2.3424530959738945 4 43 2.48591654789292 4 44 2.659113973513758 4 45 3.0552884513420047 4 46 2.8017489282900776 4 47 1.8319385654395361 4 48 2.0380926048968497 4 49 2.679735814498013 4 50 2.8469462514374406 4 51 2.681558769797629 4 52 1.9996097658618517 4 53 2.6334412104754144 4 54 2.4603636861474847 4 55 1.9652944252339424 4 56 1.0261493202120562 5 1 0.07275404687591447 5 2 0.08532445308950543 5 3 0.16108752172403484 5 4 0.1851782339941508 5 5 0.17447283382193746 5 6 0.3981361871211888 5 7 0.40452755175004285 5 8 0.36962079250621827 5 9 0.5164746729478011 5 10 0.09713169747949024 5 11 0.43401965629776385 5 12 0.35912912487319915 5 13 0.7651956782105742 5 14 -0.25145949449067473 5 15 0.18324687460629008 5 16 0.8078856986155998 5 17 0.6983174118322069 5 18 0.9422548307571006 5 19 0.40307918684597865 5 20 0.7625496739561655 5 21 1.4365382665617048 5 22 0.6063796776430266 5 23 0.8027560791706918 5 24 0.604734794801864 5 25 1.2177033348675863 5 26 2.091518553826404 5 27 2.3456489736089523 5 28 0.9579644459785186 5 29 2.369507500959692 5 30 2.200862574112861 5 31 3.029795422328874 5 32 2.978139362053028 5 33 2.3506473389693383 5 34 2.8375823004018272 5 35 3.392773525867483 5 36 2.6069937017572613 5 37 3.0024519097899542 5 38 2.813786475434739 5 39 2.9995316985486298 5 40 2.5772404383833702 5 41 2.9192743117462747 5 42 3.3122111604900226 5 43 3.391158483376792 5 44 3.5290736509331104 5 45 4.1087158706968445 5 46 2.8546723153868534 5 47 2.847563565439536 5 48 2.4045240565097536 5 49 1.897981782239949 5 50 2.6055147998245367 5 51 3.172486189152469 5 52 3.690133959410236 5 53 3.1732597588625104 5 54 3.016311266792645 5 55 3.5126734574920064 5 56 2.4878428685991523 6 1 -0.45950401764021365 6 2 -0.6082239340072633 6 3 -0.7905253815017659 6 4 -0.508370153102625 6 5 -0.7448820048877423 6 6 -0.3276702644917151 6 7 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0.5088538880201803 6 48 1.1948466371549138 6 49 0.9302398467560771 6 50 1.7184180256309887 6 51 1.285389414958921 6 52 0.7062629916683036 6 53 1.2055178233786386 6 54 1.2582467506636128 6 55 2.061060554266202 6 56 1.681391255695928 7 1 -0.41428984524465307 7 2 -0.9744322893747963 7 3 -0.3246343185721017 7 4 -0.9221723741496888 7 5 -1.0724864469818698 7 6 -0.5505682020908722 7 7 -0.6663344260394908 7 8 -0.4587768806825707 7 9 -0.30875007586235625 7 10 -0.8383521734882535 7 11 -1.2047957852675424 7 12 -0.04330345048375506 7 13 0.04229774061141711 7 14 0.10602331883561078 7 15 -0.7636066420515633 7 16 -0.39306617869799254 7 17 -1.6544060149261242 7 18 -0.06356219727040013 7 19 -0.7317700992460345 7 20 -1.5959907808296663 7 21 -0.3902729444377613 7 22 -1.791557921510858 7 23 -1.6782063745574973 7 24 -0.7823619793916876 7 25 -0.9134442061160186 7 26 -1.0848431595527614 7 27 -0.37037429450315784 7 28 0.09836634973314773 7 29 -2.0905665339424786 7 30 -1.0215065427565193 7 31 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-0.38126925442596615 8 16 -0.28888849493278457 8 17 0.21444644409027092 8 18 0.571287088821613 8 19 0.24178886426533097 8 20 0.7786787062142295 8 21 0.726860847206865 8 22 1.1870248389333469 8 23 0.9801754340093964 8 24 1.5240896335115366 8 25 1.637058173577266 8 26 1.1882927473747884 8 27 1.3133909090928242 8 28 1.9257063814623905 8 29 1.9178945977338842 8 30 1.3460238644354376 8 31 1.4491502610385538 8 32 1.0587845233433555 8 33 1.7216150809048187 8 34 2.1279048810469874 8 35 1.7959993323190986 8 36 1.8650582178862933 8 37 2.1153551355964026 8 38 2.3783026044669953 8 39 1.531789763064758 8 40 2.28691785773821 8 41 2.3225001181978904 8 42 1.9896305153287344 8 43 2.0685778382155036 8 44 2.5935897799653738 8 45 1.770006193277485 8 46 1.8546723153868534 8 47 1.3959506622137283 8 48 1.8400079274774939 8 49 2.204433395143173 8 50 3.1055147998245367 8 51 2.2531313504427892 8 52 1.7223920239263677 8 53 1.8506791137012222 8 54 0.6614725571152249 8 55 0.8191250703952342 8 56 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2.7884886861474847 119 55 2.0746694252339424 119 56 0.4480243202120562 120 1 -0.7331935337692457 120 2 -0.28513925658791095 120 3 -0.4755052202114456 120 4 -0.578934669231657 120 5 -0.7453860371458063 120 6 -0.7152710709433308 120 7 -1.382266803088669 120 8 -0.9262461429776536 120 9 -0.6604406496328465 120 10 -0.5571021734882535 120 11 0.12051159178163573 120 12 -0.21748377835260868 120 13 -0.21615512824104144 120 14 -0.21416110739389893 120 15 -0.6836886092646779 120 16 -0.3715497852553682 120 17 -0.7543035559097291 120 18 -0.624781459565483 120 19 -0.18361436154111743 120 20 -0.8508575841083541 120 21 -0.34672786247055143 120 22 -0.44755177396987733 120 23 -0.030409243409955877 120 24 0.6551380206083124 120 25 -0.2041716651324137 120 26 -0.1816669300445639 120 27 0.19494332844766404 120 28 0.018448316946262366 120 29 -0.09722637000805179 120 30 0.43725370314511736 120 31 1.052980906199842 120 32 0.9685627491498039 120 33 1.0336110486467547 120 34 1.2206468165308593 120 35 1.8423703000610345 120 36 1.5041711211121012 120 37 1.3482180388222105 120 38 1.783040507692803 120 39 1.306991375967982 120 40 1.4527444706414343 120 41 -0.11953617212469325 120 42 1.1393280959738945 120 43 0.6890415478929199 120 44 0.4091139735137581 120 45 0.9615384513420047 120 46 0.5517489282900776 120 47 0.7694385654395361 120 48 0.27246760489684974 120 49 0.992235814498013 120 50 1.0188212514374406 120 51 1.806558769797629 120 52 0.4996097658618517 120 53 1.7115662104754144 120 54 2.1478636861474847 120 55 1.7777944252339424 120 56 0.6198993202120562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 13.0 4 17.0 5 9.0 6 45.0 7 111.0 8 57.0 9 79.0 10 136.0 11 122.0 12 163.0 13 251.0 14 304.0 15 263.0 16 320.0 17 383.0 18 511.0 19 502.0 20 624.0 21 624.0 22 609.0 23 754.0 24 749.0 25 806.0 26 1001.0 27 999.0 28 1237.0 29 1386.0 30 1750.0 31 2015.0 32 2482.0 33 3094.0 34 4134.0 35 5556.0 36 8143.0 37 10978.0 38 11140.0 39 7507.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 54.309181407902955 33.07309428102915 3.1489892760226956 9.468735035045203 11 30.088084630900724 25.842028123231415 25.27172730043099 18.79815994543687 12 30.884764406263148 16.435692414853943 27.72126365892238 24.95827951996053 13 21.606129645484756 18.959237277067523 30.411690441293842 29.022942636153882 14 19.45117615475033 27.084210068058802 33.11517754785157 20.349436229339293 15 26.080016252847877 28.181277299705414 26.75479966913845 18.983906778308253 16 29.72094440655338 27.4992381477558 24.872661839183873 17.907155606506944 17 30.91814079029473 27.444094556747107 21.549534907344256 20.08822974561391 18 29.137583259566686 27.61968335969584 22.79461914643526 20.448114234302217 19 25.302201390198952 30.549549418815573 25.203523385236025 18.944725805749446 20 25.116454557327568 30.18821378299546 25.3863679238438 19.308963735833178 21 24.424257375455298 25.940706128194336 28.824135479096224 20.81090101725414 22 21.94923740004934 25.998055406405552 27.732226559665644 24.320480633879466 23 23.233539885936523 30.782626362303905 26.421802667285842 19.562031084473727 24 22.59726313650941 32.46216133853812 24.37782066723745 20.562754857715024 25 22.840885998780735 32.51661973466485 20.374778645455336 24.26771562109908 26 25.141263454526968 32.019232165942796 21.984806007872987 20.85469837165725 27 19.74488980576177 29.75607630061163 21.822381706448944 28.67665218717766 28 21.882219606817486 32.041613995321335 23.53718960231318 22.538976795548 29 27.296778695197116 27.965461100127918 22.711943249215025 22.025816955459938 30 22.470798725762577 30.23841040336306 25.668029150362926 21.62276172051144 31 28.920798417132215 27.810754189944138 22.04085195530726 21.227595437616387 32 26.47974624607147 29.45378884879525 25.437958328483294 18.628506576649983 33 27.583847676720623 27.24321649004309 24.81221614067777 20.36071969255852 34 23.208827040088504 29.622405310198257 26.374858074470875 20.793909575242363 35 22.817951480909805 33.10277543175001 25.634155575618138 18.44511751172205 36 24.231385691388603 33.0846568556025 23.85691388605566 18.82704356695323 37 26.5770423991727 32.36523588272136 22.8469056323463 18.210816085759646 38 24.532454848913314 32.688074865530666 23.02668979490693 19.752780490649098 39 24.93656857884453 31.21846656362099 22.250575986467965 21.59438887106652 40 24.1556395255533 30.254001137971787 23.20878864362517 22.381570692849746 41 21.810435391619006 33.372242325201356 24.88473211159099 19.932590171588654 42 21.727072355456947 30.7536236114963 24.77776642484929 22.74153760819746 43 22.02659892845151 30.30701178119389 23.77698944510139 23.889399845253216 44 23.109262097657105 27.780453981461207 24.91350996277644 24.196773958105247 45 24.929182606664135 29.8732587682154 23.03098443477499 22.16657419034547 46 22.378143161706728 30.800794369323327 23.29225197862212 23.52881049034783 47 21.84541203974284 30.35359438924605 24.482758620689655 23.318234950321447 48 22.46663840858266 28.89048043614891 26.689273133870238 21.953608021398193 49 25.508758089728417 29.514543249916 24.792917561467327 20.183781098888257 50 20.735218884747496 30.146897610173205 25.3994007162172 23.71848278886209 51 21.449343133959754 30.677616869547425 27.049144393622775 20.823895602870042 52 21.7483478565998 30.048540850342125 25.53365693900228 22.669454354055794 53 21.006609346669006 31.27449260104112 26.580686670176057 21.138211382113823 54 20.44746654968195 30.531549316370548 27.389047305695698 21.63193682825181 55 20.99803961728648 30.16648623343184 24.09515171021447 24.740322439067207 56 21.830203030834944 31.060207126557838 23.973143759873615 23.136446082733602 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 129.5 13 811.0 14 1363.0 15 1657.0 16 1951.0 17 1540.5 18 1130.0 19 827.5 20 525.0 21 483.0 22 361.5 23 282.0 24 345.0 25 408.0 26 398.0 27 388.0 28 517.0 29 716.0 30 786.0 31 1029.5 32 1273.0 33 1125.5 34 978.0 35 1146.0 36 1314.0 37 1586.5 38 1731.5 39 1604.0 40 1601.0 41 1598.0 42 1638.0 43 1678.0 44 1929.0 45 2180.0 46 2287.0 47 2442.0 48 2490.0 49 2325.0 50 2160.0 51 2386.5 52 2613.0 53 2791.5 54 3243.0 55 3516.0 56 3586.0 57 3656.0 58 4023.5 59 4391.0 60 4672.0 61 4953.0 62 4984.0 63 4864.5 64 4714.0 65 3765.0 66 2816.0 67 2486.5 68 2157.0 69 1909.0 70 1661.0 71 1276.5 72 760.0 73 628.0 74 589.0 75 550.0 76 424.5 77 299.0 78 242.0 79 133.0 80 81.0 81 66.0 82 51.0 83 41.5 84 32.0 85 21.0 86 10.0 87 7.5 88 6.0 89 7.0 90 4.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.002902294263615388 23 0.002902294263615388 24 0.0 25 0.0246695012407308 26 0.0986780049629232 27 0.11318947628100014 28 0.12770094759907707 29 0.1726865086851156 30 0.2379881296164618 31 0.2539507480663465 32 0.265559925120808 33 0.3119966333386542 34 0.3090943390750388 35 0.34392187023842347 36 0.40777234403796203 37 0.3685913714791543 38 0.44695331659676973 39 0.48323199489196206 40 0.5340221445052313 41 0.5441801744278852 42 0.583361146986693 43 0.59787261830477 44 0.5891657355139237 45 0.6167375310182699 46 0.6225421195455008 47 0.6834902990814238 48 0.7168666831130008 49 0.6675276806315392 50 0.7197689773766163 51 0.6965506232676931 52 0.7473407728809625 53 0.758949949935424 54 0.763303391330847 55 0.8082889524168855 56 0.7937774810988085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 68911.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.45216293479996 #Duplication Level Percentage of deduplicated Percentage of total 1 62.49423272123281 19.655787900335213 2 11.55301282642798 7.267344836092931 3 6.122543139245178 5.777016731726429 4 4.240103349635508 5.334416856525083 5 3.0312817200332196 4.767018327988275 6 2.3668912060533356 4.466630871704082 7 1.776321860293439 3.9108415202217355 8 1.508720125495986 3.7962008968089274 9 1.1949801605610408 3.382623964243735 >10 5.365876164990311 27.72706824744961 >50 0.22607732767371044 4.706070148452351 >100 0.11073175232998063 6.7739548112783154 >500 0.009227646027498385 2.4350248871733102 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 908 1.317641595681386 No Hit TTCCGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 770 1.1173832914919244 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 479 0.6950994761358854 No Hit TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 458 0.6646253863679239 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 366 0.531119850241616 No Hit TTCCGCGGGGATAGCTGGAGTTGTAACTCTGTAGTACACAAGATACATGCACAAGG 308 0.44695331659676973 No Hit TTCCGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 240 0.34827531163384656 No Hit TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 238 0.3453730173702312 No Hit TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 234 0.33956842884300037 No Hit TTCCGCGGGATTGTAAACATAAACAGCATGGAGAGGTTAAATACCTGTTTTCAGAT 234 0.33956842884300037 No Hit TTCCGCGGGGGTTTGAGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAA 211 0.3061920448114234 No Hit TTCCGCGGGACCCTAGGGGATGCTTGGATATTTGCAATGCAGCCCTCCTCTGGGCT 172 0.24959730667092334 No Hit TTCCGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 162 0.23508583535284644 No Hit TTCCGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 151 0.2191232169029618 No Hit TTCCGCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 150 0.21767206977115408 No Hit TTCCGCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 147 0.213318628375731 No Hit TTCCGCGGGGTCTGGGACCCCTGGAGAGTTCTGCCTGCTGAGCCAAACCTCCTCTA 132 0.1915514213986156 No Hit TTCCGCGGGTGAATAGCCATTCGAACTTGGGAAAGTGACCTAATATTTCTGAGCCT 118 0.1712353615533079 No Hit TTCCGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 116 0.1683330672896925 No Hit TTCCGCGGGAATTTGGGTTCCTTAGCCATTCCCAGAGAGGGGGAGGTGAGGCCCAG 114 0.16543077302607712 No Hit TTCCGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 113 0.1639796258942694 No Hit TTCCGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.15672389023523095 No Hit TTCCGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.15382159597161557 No Hit TTCCGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA 105 0.15237044883980788 No Hit TTCCGCGGGACATATAAATGTATTGTAGCTTTCCGGTGTCAGCCACGGTGTATTTT 103 0.1494681545761925 No Hit TTCCGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 103 0.1494681545761925 No Hit TTCCGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 98 0.142212418917154 No Hit TTCCGCGGGAACATGGCATTGTTACCAACTGGGACGACATGGAGAAGATCTGGCAC 92 0.13350553612630783 No Hit TTCCGCGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTACTTT 88 0.12770094759907707 No Hit TTCCGCGGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCT 82 0.1189940648082309 No Hit TTCCGCGGGCTTTTGAAATTTTTTTCAGGTGACTTAATGTATCTTAATAACTACTA 82 0.1189940648082309 No Hit TTCCGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 82 0.1189940648082309 No Hit TTCCGCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.11754291767642322 No Hit TTCCGCGGGATTTTCACTGTTAAAAAAAAATTTCATCTTTTTTGGAATTTTAAAAT 81 0.11754291767642322 No Hit TTCCGCGGGACTTCAGCACTGCTGCTGTAAAGAATTCCCTGAATTGTTGAATTGTT 79 0.11464062341280783 No Hit TTCCGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 78 0.11318947628100014 No Hit TTCCGCGGGGAAGAGACCCGAGAGGGACTGGAAAGATGACTAGGTTAAGAGCACTG 78 0.11318947628100014 No Hit TTCCGCGGGGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTT 78 0.11318947628100014 No Hit TTCCGCGGGGTTGTATGATGCCACTTTCCATGTGTATGTGATACCACTGTACATGT 75 0.10883603488557704 No Hit TTCCGCGGGGTGGAGCTGAGTATGTGGCTGAGGGCCTGAGGCAGGAGCAGGGGGCG 75 0.10883603488557704 No Hit TTCCGCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 73 0.10593374062196166 No Hit TTCCGCGGGATTTTGGATGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATG 72 0.10448259349015397 No Hit TTCCGCGGGATTTTTGAAATAAATTTTTTAATTTATAGAGTATTTCTGAGGGGTGG 72 0.10448259349015397 No Hit TTCCGCGGGGTGGGTCATCAGCCCATGCCTCACCCTGGCAGTGTGCTCTAACTGAG 71 0.10303144635834627 No Hit TTCCGCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAG 69 0.10012915209473087 No Hit TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 69 0.10012915209473087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.004353441395423082 0.0 0.0 0.0 0.0 11 0.013060324186269246 0.0 0.0 0.0 0.0 12 0.015962618449884634 0.0 0.0 0.0 0.0 13 0.01886491271350002 0.0 0.0 0.0 0.0 14 0.02902294263615388 0.0 0.0 0.0 0.0 15 0.03192523689976927 0.0 0.0 0.0 0.0 16 0.03192523689976927 0.0 0.0 0.0 0.0 17 0.03192523689976927 0.0 0.0 0.0 0.0 18 0.03337638403157696 0.0 0.0 0.0 0.0 19 0.03627867829519235 0.0 0.0 0.0 0.0 20 0.042083266822423124 0.0 0.0 0.0 0.0 21 0.04353441395423082 0.0 0.0 0.0 0.0 22 0.04353441395423082 0.0 0.0 0.0 0.0 23 0.04933900248146159 0.0 0.0 0.0 0.0 24 0.05659473814050007 0.0 0.0 0.0 0.0 25 0.059497032404115455 0.0 0.0 0.0 0.0 26 0.06239932666773084 0.0 0.0 0.0 0.0 27 0.06239932666773084 0.0 0.0 0.0 0.0 28 0.10158029922653858 0.0 0.0 0.0 0.0 29 0.10303144635834627 0.0 0.0 0.0 0.0 30 0.13060324186269245 0.0 0.0 0.0 0.0 31 0.13350553612630783 0.0 0.0 0.0 0.0 32 0.13350553612630783 0.0 0.0 0.0 0.0 33 0.13495668325811555 0.0 0.0 0.0 0.0 34 0.13495668325811555 0.0 0.0 0.0 0.0 35 0.1726865086851156 0.0 0.0 0.0 0.0 36 0.17413765581692328 0.0 0.0 0.0 0.0 37 0.17558880294873097 0.0 0.0 0.0 0.0 38 0.17849109721234635 0.0 0.0 0.0 0.0 39 0.17994224434415407 0.0 0.0 0.0 0.0 40 0.18284453860776945 0.0 0.0 0.0 0.0 41 0.18429568573957714 0.0 0.0 0.0 0.0 42 0.18574683287138483 0.0 0.0 0.0 0.0 43 0.1886491271350002 0.0 0.0 0.0 0.0 44 0.1886491271350002 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGAGG 15 0.00818727 50.321346 50 GCCTGCA 15 0.00818727 50.321346 49 CATGCAC 25 1.998491E-5 50.28446 46 CATATAG 15 0.008211063 50.284454 47 ATATAGA 15 0.008211063 50.284454 48 GTAGTTT 15 0.008211063 50.284454 47 TAACTAC 15 0.008211063 50.284454 48 AAATACC 45 1.2732926E-10 50.21084 39 GTTAAAT 45 1.2732926E-10 50.21084 36 AATACCT 45 1.2732926E-10 50.21084 40 CAGCGTA 15 0.008258805 50.210835 36 AGCGTAG 15 0.008258805 50.210835 37 CATATGG 15 0.008258805 50.210835 40 GTCTGGT 15 0.008258805 50.210835 34 GCGGTAG 15 0.008258805 50.210835 38 TTTGTCG 15 0.008258805 50.210835 37 ATTACAT 20 4.0707015E-4 50.210835 34 TTGTCGG 15 0.008258805 50.210835 38 TGCGGTA 15 0.008258805 50.210835 37 TACATGC 25 2.0159187E-5 50.210835 44 >>END_MODULE