FastQCFastQC Report
Tue 31 May 2016
SRR212866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165335
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18931.1449481356034716No Hit
GACACTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13680.827411014001875No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT11100.6713641999576617No Hit
GACACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5150.31148879547585206No Hit
GACACTGGGGACAGTCTCTCCTCTCAAAACTATATATATGCCTGCTCAGCAGCAACAA4550.27519883872138384No Hit
GACACTGGGACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCTGT4510.272779508271086No Hit
GACACTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.24616687331780931No Hit
GACACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA3970.2401185471920646No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3580.2165300753016603No Hit
GACACTGGGGGCTTAGGGTACACTCAGACCCTAAAACCAGGTATCAAACTGACGTTGT3330.20140925998729853No Hit
GACACTGGGACTGAGGTTGTCTCTTTACTGCCTGGCATGACATCTAAAAGCTCACTGC3180.19233677079868147No Hit
GACACTGGGCAAACAGAACTGTAGCCACGACTGGGGAATTTTTGTGAGATACAAGACG3010.18205461638491546No Hit
GACACTGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2940.1778207880968942No Hit
GACACTGGGATTTTGGTGACCCCAACCATAAATTATTTTTATGATTTGGCTACTGTTA2920.17661112287174524No Hit
GACACTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.1735869598088729No Hit
GACACTGGGAATCCAATGCATACTAAAGAAAGCTTAGTTACTGTATTGTTCAGTGAAA2800.1693531315208516No Hit
GACACTGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGTAT2650.16028064233223455No Hit
GACACTGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGAAA2480.14999848791846856No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2450.14818399008074518No Hit
GACACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA2400.1451598270178728No Hit
GACACTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.13366800737895787No Hit
GACACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2190.13245834215380894No Hit
GACACTGGGGAGGACTTCCAATAACAGTGTATTCATCAGTAAATACCTCACATTTAGC2100.1270148486406387No Hit
GACACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2080.12580518341548974No Hit
GACACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2080.12580518341548974No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1990.12036168990231953No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1940.11733752683944718No Hit
GACACTGGGGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAA1940.11733752683944718No Hit
GACACTGGGATCCTGGGCAGCCATTTTGATGCGGCCCAGATTTCTATTATTTTATACA1890.11431336377657483No Hit
GACACTGGGGGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAA1890.11431336377657483No Hit
GACACTGGGCATCATTTGAGTTTTGTTGAGAGAGTCACAAAGGGCTTATGAAAATCAC1820.11007953548855354No Hit
GACACTGGGAGTTGTGAGCCACTATGTGGTTGCTGGGATTTGAACTTTGGACCTCCGG1770.1070553724256812No Hit
GACACTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1760.10645053981310673No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA1760.10645053981310673No Hit
GACACTGGGGCTGCCTGGTGGTTCACTCTGCTCCCTCAAAACTCCTTTGTTTTCAATA1750.10584570720053225No Hit
GACACTGGGATTTCCCATCCCTGACCAGTTCTGGAGAGCGGTGTGTATAGACACTGGG1750.10584570720053225No Hit
GACACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1720.10403120936280884No Hit
GACACTGGGGATCTTTAAACATTAAACTCAACTCTTAAACTATTAACTATTAAAAAGT1720.10403120936280884No Hit
GACACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1700.1028215441376599No Hit
GACACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1690.10221671152508542No Hit
GACACTGGGACAGAGGAATGGATACAGAAAATGTGGTACATTTACACATTGGAGTACT1670.10100704629993648No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAC150.007165552.08007451
ATCCGCG150.007165552.08007452
GTCAATC150.007165552.08007445
GCAAGCG459.094947E-1152.0800749
TCACGGT150.00717410152.06428541
CAAACGT150.00717410152.06428544
GCGTTCG150.00717410152.06428541
ACCTAGC150.00717410152.06428547
GTAGACG150.00717410152.06428548
ACGTATA150.00717410152.06428547
CCGGTAA150.00717410152.06428543
CACAATT150.00717410152.06428542
TAGGAAC150.00717410152.06428542
CGGTAAA150.00717410152.06428544
CGAAGGG150.00717410152.06428542
TACGTAG251.6369591E-552.0642841
CGAGTTA203.4180956E-452.06427841
CGTATAC203.4180956E-452.06427848
TACACCG150.0071827152.04850439
GATAGTT150.0071827152.04850440
Produced by FastQC (version 0.11.5)