FastQCFastQC Report
Tue 31 May 2016
SRR212865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166751
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18461.1070398378420518No Hit
GACACTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.8017942920882034No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT11780.7064425400747222No Hit
GACACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.2998482767719534No Hit
GACACTGGGACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCTGT4420.2650658766664068No Hit
GACACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA4360.26146769734514336No Hit
GACACTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.24947376627426524No Hit
GACACTGGGGACAGTCTCTCCTCTCAAAACTATATATATGCCTGCTCAGCAGCAACAA4160.24947376627426524No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3760.22548590413250894No Hit
GACACTGGGGGCTTAGGGTACACTCAGACCCTAAAACCAGGTATCAAACTGACGTTGT3230.1937019867946819No Hit
GACACTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.18770502125924282No Hit
GACACTGGGCAAACAGAACTGTAGCCACGACTGGGGAATTTTTGTGAGATACAAGACG2950.1769104832954525No Hit
GACACTGGGAATCCAATGCATACTAAAGAAAGCTTAGTTACTGTATTGTTCAGTGAAA2920.17511139363482078No Hit
GACACTGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGTAT2800.1679150349922939No Hit
GACACTGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2750.16491655222457435No Hit
GACACTGGGACTGAGGTTGTCTCTTTACTGCCTGGCATGACATCTAAAAGCTCACTGC2630.15772019358204747No Hit
GACACTGGGATTTTGGTGACCCCAACCATAAATTATTTTTATGATTTGGCTACTGTTA2580.15472171081432795No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2490.1493244418324328No Hit
GACACTGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGAAA2450.14692565561825716No Hit
GACACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2200.13193324177965948No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2180.13073384867257168No Hit
GACACTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.13013415211902776No Hit
GACACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2090.12533657969067652No Hit
GACACTGGGGAGGACTTCCAATAACAGTGTATTCATCAGTAAATACCTCACATTTAGC2050.1229377934765009No Hit
GACACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA2010.12053900726232526No Hit
GACACTGGGATCCTGGGCAGCCATTTTGATGCGGCCCAGATTTCTATTATTTTATACA2000.11993931070878136No Hit
GACACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC1970.11814022104814964No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1940.11634113138751792No Hit
GACACTGGGGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAA1920.11514173828043012No Hit
GACACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1810.10854507619144714No Hit
GACACTGGGCATCATTTGAGTTTTGTTGAGAGAGTCACAAAGGGCTTATGAAAATCAC1790.10734568308435931No Hit
GACACTGGGATTTCCCATCCCTGACCAGTTCTGGAGAGCGGTGTGTATAGACACTGGG1780.1067459865308154No Hit
GACACTGGGGGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAA1770.1061462899772715No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA1750.10494689687018369No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.10494689687018369No Hit
GACACTGGGGAACTTCTATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAA1710.10254811065600807No Hit
GACACTGGGACAGAGGAATGGATACAGAAAATGTGGTACATTTACACATTGGAGTACT1670.10014932444183243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGG150.007100031752.20126327
AGGCGAT150.007100031752.20126326
ACGATTC150.007100031752.20126324
TCTCGTA150.007100031752.20126324
CAGTAGG150.007100031752.20126325
TCAGTAG150.007100031752.20126324
AACGCGA203.3791334E-452.18554723
TAACAGC203.3841634E-452.1698321
ATTAAAC150.007116971552.16982721
TTACTGG150.007116971552.16982721
TAGACGG203.4347936E-452.01321449
CTAGCTA203.4347936E-452.01321451
TGTATCG150.007202123852.01320649
GTATCGC150.007202123852.01320650
TTACGAT150.007202123852.01320652
ACTGACG353.8578946E-851.99760448
TAACTAT150.007210680351.99759744
ATAGGTA203.4449916E-451.98240
CCGTTAA150.007219244751.98199530
GTGTTAT203.4500996E-451.9664136
Produced by FastQC (version 0.11.5)