FastQCFastQC Report
Tue 31 May 2016
SRR212864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167846
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16200.9651704538684269No Hit
GACACTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12870.7667743050176948No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10330.615445110398818No Hit
GACACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4590.2734649619293877No Hit
GACACTGGGACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCTGT4520.2692944723139068No Hit
GACACTGGGGACAGTCTCTCCTCTCAAAACTATATATATGCCTGCTCAGCAGCAACAA3900.23235585000536207No Hit
GACACTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3800.22639800769753224No Hit
GACACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA3690.21984438115891947No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC3480.2073329123124769No Hit
GACACTGGGGGCTTAGGGTACACTCAGACCCTAAAACCAGGTATCAAACTGACGTTGT2870.17099007423471516No Hit
GACACTGGGCAAACAGAACTGTAGCCACGACTGGGGAATTTTTGTGAGATACAAGACG2710.16145752654218748No Hit
GACACTGGGACTGAGGTTGTCTCTTTACTGCCTGGCATGACATCTAAAAGCTCACTGC2650.1578828211574896No Hit
GACACTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.15609546846514066No Hit
GACACTGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGTAC2550.15192497884965983No Hit
GACACTGGGATTTTGGTGACCCCAACCATAAATTATTTTTATGATTTGGCTACTGTTA2510.1495418419265279No Hit
GACACTGGGAATCCAATGCATACTAAAGAAAGCTTAGTTACTGTATTGTTCAGTGAAA2480.14775448923417894No Hit
GACACTGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGTAT2370.14120086269556617No Hit
GACACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2240.13345566769538744No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2240.13345566769538744No Hit
GACACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA2200.1310725307722555No Hit
GACACTGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGAAA2140.12749782538755763No Hit
GACACTGGGGAGGACTTCCAATAACAGTGTATTCATCAGTAAATACCTCACATTTAGC2050.12213576731051083No Hit
GACACTGGGATTTCCCATCCCTGACCAGTTCTGGAGAGCGGTGTGTATAGACACTGGG2040.12153998307972784No Hit
GACACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2030.12094419884894486No Hit
GACACTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.12034841461816188No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1970.11736949346424698No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA1910.11379478807954911No Hit
GACACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1830.10902851423328529No Hit
GACACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1800.10724116154093634No Hit
GACACTGGGGAACTTCTATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAA1780.10604959307937037No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA1740.10366645615623846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT203.3277637E-452.3477759
ATCCGGG203.3277637E-452.34777510
GAACATA203.3277637E-452.3477759
GGATACT150.007021798852.347778
GGATACG150.007021798852.347778
GTCCGTT150.007021798852.3477710
GTATCTC150.007021798852.347779
GCTAGCA150.007021798852.3477710
TGGGCGA503.6379788E-1252.347776
AGTACAC150.007021798852.3477710
GGCTCGA150.007021798852.347779
AGGTATC307.884646E-752.06582639
TAGGTAT150.007173427452.06582639
GTAGGTA150.007173427452.06582638
CCAGGTA307.917297E-752.03468737
GATCCTA251.6425758E-552.03468737
TAGTTAC203.4278395E-452.03468735
ATATAGT150.00719042452.03468735
CTCATAC150.00719042452.03468736
TTAGTTA203.4278395E-452.03468734
Produced by FastQC (version 0.11.5)