FastQCFastQC Report
Tue 31 May 2016
SRR212863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175108
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16890.9645475934851634No Hit
GACACTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13630.7783767731914019No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT13270.7578180323000662No Hit
GACACTGGGACTGAGGTTGTCTCTTTACTGCCTGGCATGACATCTAAAAGCTCACT6030.34435890992987184No Hit
GACACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4900.2798273065765128No Hit
GACACTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.23756767252210062No Hit
GACACTGGGGACAGTCTCTCCTCTCAAAACTATATATATGCCTGCTCAGCAGCAAC4140.23642552025035976No Hit
GACACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC3470.19816341914704066No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3230.18445759188615027No Hit
GACACTGGGATTTTGGTGACCCCAACCATAAATTATTTTTATGATTTGGCTACTGT2970.16960961235351896No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2890.1650410032665555No Hit
GACACTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.16218562258720332No Hit
GACACTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2700.15419055668501724No Hit
GACACTGGGATTAAGGAAATACCAGAATGACAGGACCATTGCAAGGGTATAATTTC2680.15304840441327638No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2670.15247732827740595No Hit
GACACTGGGATTTGTTTTGCTTTTGTTTTTTTCCTCGTGTAACATTGGTGAAGGAT2600.1484797953263129No Hit
GACACTGGGGGCTTAGGGTACACTCAGACCCTAAAACCAGGTATCAAACTGACGTT2580.14733764305457203No Hit
GACACTGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGT2560.14619549078283117No Hit
GACACTGGGGAAACACTTCGGATTCTACTGTAGCAGCCCAGAGCAGATTAATGAAA2560.14619549078283117No Hit
GACACTGGGCAAACAGAACTGTAGCCACGACTGGGGAATTTTTGTGAGATACAAGA2520.14391118623934943No Hit
GACACTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.14105580555999728No Hit
GACACTGGGAATCCAATGCATACTAAAGAAAGCTTAGTTACTGTATTGTTCAGTGA2380.13591612033716335No Hit
GACACTGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGT2260.12906320670671814No Hit
GACACTGGGGGTATCAGCAGATCATATGGCAGCATGAATCCAAGAGCCCACTGGGA2090.11935491239692077No Hit
GACACTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.11364415103821643No Hit
GACACTGGGCATCATTTGAGTTTTGTTGAGAGAGTCACAAAGGGCTTATGAAAATC1980.11307307490234597No Hit
GACACTGGGATCCTGGGCAGCCATTTTGATGCGGCCCAGATTTCTATTATTTTATA1930.1102176942229938No Hit
GACACTGGGGGGTGTTGTACATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAG1900.1085044658153825No Hit
GACACTGGGAGTTGTGAGCCACTATGTGGTTGCTGGGATTTGAACTTTGGACCTCC1890.10793338967951208No Hit
GACACTGGGATTTCCCATCCCTGACCAGTTCTGGAGAGCGGTGTGTATAGACACTG1850.10564908513603034No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.10450693286428946No Hit
GACACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1800.10279370445667817No Hit
GACACTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.1016515521849373No Hit
GACACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1760.10050939991319643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC252.0164556E-550.26332550
ACGTCAT150.0082460450.2633250
CCTAGTA150.00825543850.24884849
CGTCACA150.00825543850.24884849
AATATAC150.00826484350.2343844
ATATACC150.00826484350.2343845
AATTATC150.00826484350.2343845
CGTAAGT150.00826484350.2343844
GCGTCAC150.00826484350.2343848
GTTGGCC150.00826484350.2343846
TCGCGTG204.0788067E-450.2343845
AATAACC150.00826484350.2343845
ACCGGTA204.0904153E-450.20546742
ATCTAAA900.050.20546742
GTGATAC150.00828367850.20546742
TCCACCT150.00828367850.20546742
AAGGGTA301.01363E-650.20546743
CACAATT150.00828367850.20546742
ACACGAC150.00830254550.17658641
ACACCGT204.1020502E-450.17658640
Produced by FastQC (version 0.11.5)