FastQCFastQC Report
Tue 31 May 2016
SRR212862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162167
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15630.9638212459994944No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12100.7461444066918671No Hit
GACACTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11120.6857128762325257No Hit
GACACTGGGACTGAGGTTGTCTCTTTACTGCCTGGCATGACATCTAAAAGCTCACT5200.32065710039650486No Hit
GACACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.2522091424272509No Hit
GACACTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3890.23987617702738534No Hit
GACACTGGGGACAGTCTCTCCTCTCAAAACTATATATATGCCTGCTCAGCAGCAAC3690.22754321162751978No Hit
GACACTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3680.2269265633575265No Hit
GACACTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC3180.19609414985786258No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA3120.1923942602379029No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3100.19116096369791635No Hit
GACACTGGGATTAAGGAAATACCAGAATGACAGGACCATTGCAAGGGTATAATTTC3000.18499448099798357No Hit
GACACTGGGGAAACACTTCGGATTCTACTGTAGCAGCCCAGAGCAGATTAATGAAA2770.17081157078813816No Hit
GACACTGGGATGACCTTTTTATGGCTTTCAGCACTCTCAGAGTTAATAGAGACTGG2470.1523121226883398No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2340.1442956951784272No Hit
GACACTGGGAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATGCTAGT2280.1405958055584675No Hit
GACACTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.13997915728847424No Hit
GACACTGGGATTTTGGTGACCCCAACCATAAATTATTTTTATGATTTGGCTACTGT2270.13997915728847424No Hit
GACACTGGGATTTGTTTTGCTTTTGTTTTTTTCCTCGTGTAACATTGGTGAAGGAT2260.13936250901848093No Hit
GACACTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.13689591593850783No Hit
GACACTGGGAATCCAATGCATACTAAAGAAAGCTTAGTTACTGTATTGTTCAGTGA2210.13627926766851456No Hit
GACACTGGGGGCTTAGGGTACACTCAGACCCTAAAACCAGGTATCAAACTGACGTT2210.13627926766851456No Hit
GACACTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.13381267458854146No Hit
GACACTGGGCAAACAGAACTGTAGCCACGACTGGGGAATTTTTGTGAGATACAAGA2020.12456295053864226No Hit
GACACTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1950.12024641264868931No Hit
GACACTGGGGAGGGTCCAGGCCATCCTCAGCTGCCGAGCCTCATTGTGACCTTTGT1920.11839646783870948No Hit
GACACTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1900.11716317129872292No Hit
GACACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.11469657821874982No Hit
GACACTGGGACTCTCAGAGTTAATAGAGACTGGCTTAGGAGGGCACTGGTGGCCCT1860.11469657821874982No Hit
GACACTGGGACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACA1840.11346328167876325No Hit
GACACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1780.10976339205880357No Hit
GACACTGGGGAGGACTTCCAATAACAGTGTATTCATCAGTAAATACCTCACATTTA1720.10606350243884391No Hit
GACACTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1710.10544685416885063No Hit
GACACTGGGAGTTGTGAGCCACTATGTGGTTGCTGGGATTTGAACTTTGGACCTCC1700.10483020589885735No Hit
GACACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1650.10174696454889096No Hit
GACACTGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1640.10113031627889768No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.1005136680089044No Hit
GACACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1630.1005136680089044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCA150.00826636850.2303449
GTACTAG252.0236323E-550.2303450
ACTCGCG150.00826636850.2303449
GCAAGCG355.0646122E-850.23033549
AATATAC150.00827653350.21473344
GTTAACT150.00827653350.21473343
AACTAAT150.00827653350.21473346
GATAGGC150.00827653350.21473348
ACTGATA204.085785E-450.2147345
CTGATAG204.085785E-450.2147346
AACCGGT150.00829689150.1835441
TGTGATA150.00829689150.1835441
AGACAGT150.00829689150.1835441
TAGGCTC150.00829689150.1835441
TGTGATC150.00831728750.1523939
GACGGTT150.00831728750.1523939
CTACATA150.00831728750.1523937
CTACACC150.00831728750.1523938
CCTATAA252.0423215E-550.1523939
TGTCGGG150.00831728750.1523937
Produced by FastQC (version 0.11.5)