FastQCFastQC Report
Tue 31 May 2016
SRR212861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292260
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18520.633682337644563No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14100.4824471361116814No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT13570.4643125983713132No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11580.39622254157257236No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6060.20734962020119074No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5670.19400533771299527No Hit
CGGCAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTCTTCCCA4760.16286867857387258No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4680.16013138985834532No Hit
CGGCAGGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC4000.1368644357763635No Hit
CGGCAGGGGATTCCTGTTGATATGTGTGACTTTCCAGGAGAGGATTAATCAGTGTGGC3770.12899473071922263No Hit
CGGCAGGGGGTCAATAAACTTAAAACTACTGTTGTGTCTAAAAAGTCGGTGTTGTACA3390.11599260932046807No Hit
CGGCAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3390.11599260932046807No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3340.11428180387326352No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3340.11428180387326352No Hit
CGGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.11393964278382263No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3300.11291315951549989No Hit
CGGCAGGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG3270.11188667624717717No Hit
CGGCAGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGC3220.11017587079997264No Hit
CGGCAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT3160.10812290426332717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA150.00718007752.0634249
TAGCGCA307.92641E-752.0455843
CGTCTAT150.007189817352.0455843
ACGTCTA150.007189817352.0455842
ACGTACA150.007189817352.0455845
CGCGTGA150.007189817352.0455841
CGTCCAG150.007189817352.0455847
GTACGCG150.007189817352.0455846
CGTAGCG353.8433427E-852.04557841
TAATACG150.007204445552.0188438
TCGCACG150.007204445552.0188436
GCTACCG459.276846E-1152.0188436
GCACGCG203.437252E-452.01883738
TCGATAC203.437252E-452.01883738
CTACCGT353.858986E-852.01883737
CGTCACA203.437252E-452.01883737
CGATACA203.437252E-452.01883739
AATACGT203.437252E-452.01883739
ATCGATA203.437252E-452.01883737
TCGTTAG150.007209326652.00993332
Produced by FastQC (version 0.11.5)