FastQCFastQC Report
Tue 31 May 2016
SRR212860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297633
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18430.6192189710146389No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT14170.4760896809157587No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13560.45559464172319597No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA11680.39242960290021606No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6460.2170458248917291No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5530.1857992897292975No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4950.16631220328391005No Hit
CGGCAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTCTTCCCA4690.15757661280839153No Hit
CGGCAGGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC4160.13976944760829613No Hit
CGGCAGGGGATTCCTGTTGATATGTGTGACTTTCCAGGAGAGGATTAATCAGTGTGGC3600.1209543296610255No Hit
CGGCAGGGGGTCAATAAACTTAAAACTACTGTTGTGTCTAAAAAGTCGGTGTTGTACA3470.11658653442326625No Hit
CGGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.11557858167609103No Hit
CGGCAGGGGGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGC3400.1142346446798574No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3370.1132266919326822No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3320.11154677068739019No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3310.11121078643833177No Hit
CGGCAGGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG3260.10953086519303976No Hit
CGGCAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3030.10180322746469646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT150.007158592352.1031226
CGGCTCG150.007158592352.1031222
TATTCGG203.4355957E-452.02422748
GTGCTAA150.007211210752.0067343
CACGATA150.007220808451.9892440
CATAGAG150.007220808451.9892435
TCGCTAC203.4470268E-451.9892434
TATAGTG150.007220808451.9892439
CGTCAAC150.007220808451.9892431
ATCGATA307.986382E-751.9892437
ACGTTAC150.00722561151.98050329
TAGGTCC150.007235222551.96303618
ACCAGTA150.007235222551.96303618
AATATGC203.4556197E-451.9630317
AGTATAG203.4556197E-451.9630318
GACCATA251.6608305E-551.9543049
CAGGGGA143250.050.8662534
CGGCAGG304150.050.835441
GCAGGGG304750.050.735363
GGCAGGG305150.050.6688462
Produced by FastQC (version 0.11.5)