FastQCFastQC Report
Tue 31 May 2016
SRR212859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301930
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16460.5451594740502765No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT13400.4438114794819991No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12150.40241115490345447No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA10570.35008114463617396No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.18679826449839368No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC5290.17520617361640115No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA4630.15334680223892955No Hit
CGGCAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTCTTCCCA4210.13943629318053855No Hit
CGGCAGGGGGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGAC4120.1364554698108833No Hit
CGGCAGGGGATTCCTGTTGATATGTGTGACTTTCCAGGAGAGGATTAATCAGTGTGGC3320.10995926208061471No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3260.10797204650084458No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3220.10664723611433113No Hit
CGGCAGGGGACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAGCACTGTCAG3110.1030040075514192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA150.007193501652.0392339
TCGGAAC150.007202964352.02193551
TAGCGCA203.4392325E-452.01328743
CCGATAC150.007212436352.0046549
TTGCGTC150.00721717651.99601425
CGTAGCG251.6546335E-551.9873841
ACGCCTA150.00722666251.9787535
TAGAACG251.6562608E-551.9787529
TGAAACG150.00722666251.9787536
TCGCTAC203.4505327E-451.9787534
TCGAGCA150.00722666251.9787529
TTCGCTA203.4505327E-451.9787533
TAATAGG150.007259935651.91840723
CGATTTC251.6726071E-551.8925917
AATTCGT150.007274230551.8925913
TCGAGCC150.007274230551.8925917
TCGCGAC251.6726071E-551.8925916
CCGCATA203.4903162E-451.85820819
GCCGTAA150.00729332351.85820819
CAGGGGC26350.050.9191674
Produced by FastQC (version 0.11.5)