FastQCFastQC Report
Tue 31 May 2016
SRR212858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328083
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT17630.5373640206898864No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17600.5364496179320477No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13800.4206252686058102No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13480.4108716391888638No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6200.18897656995333498No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC6030.18379495432558224No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA5370.16367809365313046No Hit
CGGCAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4800.1463044412541948No Hit
CGGCAGGGGAGATGAAGTCATTCAGAATTGCCCCATTGATGTCCGGCGTCCTCTCT4680.14264683022283997No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4450.13563640907940977No Hit
CGGCAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC4210.12832118701670003No Hit
CGGCAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTCTTCC4070.12405397414678604No Hit
CGGCAGGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCT3560.10850912726352784No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.10607071990929125No Hit
CGGCAGGGGGTCAATAAACTTAAAACTACTGTTGTGTCTAAAAAGTCGGTGTTGTA3440.10485151623217295No Hit
CGGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3350.10210830795865682No Hit
CGGCAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG3340.10180350703937723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC150.00824374450.27679448
CGTGCAC150.00824875750.26906647
CTATACT150.00826380950.2458945
TTGCGTG150.00827385450.23045344
AGTCCGT150.00831917250.16109540
TCGATAC402.6193447E-950.11496438
TCAGGCG150.00834948750.1149637
ATCGATA355.1792085E-850.1149637
TAGGGCG204.1386217E-450.09960634
ATGCGTT150.0083596150.099633
TCGAGTA150.0083596150.099633
TATGCGT150.00836974250.08425532
ACTTGCG150.00839003450.05358531
CTAGTGA150.00839003450.05358531
GTATGCG150.00839003450.05358531
GTGCGAT550.050.05358531
ACGTTAC355.2674295E-850.0076529
TTAGCGA204.176314E-450.0076527
CTCGATG150.0084205450.0076529
GCTCGAT150.0084205450.0076528