FastQCFastQC Report
Tue 31 May 2016
SRR212857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309925
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16690.5385173832378801No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT16430.5301282568363314No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13740.44333306445107684No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12130.39138501250302493No Hit
CGGCAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC9100.29361942405420666No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC6150.1984351052674034No Hit
CGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5800.18714205049608776No Hit
CGGCAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA5000.16132935387593772No Hit
CGGCAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTCTTCC4810.1551988384286521No Hit
CGGCAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC4580.14777768815035897No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4330.1397112204565621No Hit
CGGCAGGGGAGATGAAGTCATTCAGAATTGCCCCATTGATGTCCGGCGTCCTCTCT3870.1248689198999758No Hit
CGGCAGGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG3630.1171251109139308No Hit
CGGCAGGGGATTCCAAGTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCT3600.11615713479067517No Hit
CGGCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.1051867387271114No Hit
CGGCAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.1051867387271114No Hit
CGGCAGGGGATTCCTGTTGATATGTGTGACTTTCCAGGAGAGGATTAATCAGTGTG3100.10002419940308138No Hit
CGGCAGGGGGTCAATAAACTTAAAACTACTGTTGTGTCTAAAAAGTCGGTGTTGTA3100.10002419940308138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCG150.00829778350.19311547
CATAGCG150.00832445450.15238643
TCGATAC204.126717E-450.1279838
GTACGTC150.00834048750.1279837
TACCGGA150.00834048750.1279838
TCGCTAC301.0293988E-650.11172534
CGATCGG150.00835118850.11172534
AGACTCG150.00837262250.07924333
ATTAGCC204.1465764E-450.07924333
CCGATTA204.1498936E-450.0711330
CGATTAG204.1498936E-450.0711331
GATTAGC204.1498936E-450.0711332
GTGCGAT204.1498936E-450.0711331
CCGTTAA355.2137693E-850.07112530
CCACGTG150.00839409650.046829
CGATTGG150.00839947250.038728
ATCCGAT204.1631836E-450.038728
CCGTAAT150.00839947250.038728
CACGTTA355.2405085E-850.038728
CTCGGTA150.00840484950.03059827
Produced by FastQC (version 0.11.5)