FastQCFastQC Report
Tue 31 May 2016
SRR212856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453731
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61391.3530043131282632No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44010.9699579706918857No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGTGG39130.86240525774082No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT26430.5825037301837432No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23150.5102142018067973No Hit
CGTATTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12990.286292979761136No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGGGG12670.279240342846312No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGTGG12300.27108573141354675No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG11960.26359230469154626No Hit
CGTATTGGGGGAGGGGTGTTTGAACACACCCCTCCCACAGGACTCACTTTGTCCCAAC8860.195269884579189No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8360.18425013939977652No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGGGG7680.16926328595577556No Hit
CGTATTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7530.16595736240195183No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6310.1390691841641854No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG5790.12760864917759643No Hit
CGTATTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.1141645600587132No Hit
CGTATTGGGGGCAGGGTGGACAGACAGGAGCGCACGTGAGTCACCCACTGAGTCATGA4900.10799350275824222No Hit
CGTATTGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG4890.10777310785465397No Hit
CGTATTGGGGTGTGGGCTGTTCTCTCCTACTTTTGTGTGTGTGTGTGTGTGTGTGTGT4720.10402639449365372No Hit
CGTATTGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.10160205055418298No Hit
CGTATTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4610.10160205055418298No Hit
CGTATTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4600.10138165565059473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGT251.6461194E-552.03983350
CCCGCGA150.007195511452.03983352
CGCTTAG203.432438E-452.03983351
CCGGATA150.007201792752.0283547
GCGCATA203.4361792E-452.0283547
CGGATAA150.007201792752.0283548
ATATTCG203.4380512E-452.02261442
ACGGTAC353.8631697E-852.0226144
CGCACAT150.00720807852.01687640
CGCTATA150.00721122352.0111437
TCGGGTC203.4436717E-452.005428
GCGGCTA150.00721436852.005429
CGCACGT401.88993E-952.005431
AATAGCG203.4436717E-452.005432
CGAACGG203.445547E-451.99966427
GCGAACG203.445547E-451.99966426
TCGCACA203.4474226E-451.99393524
CGCGAAC203.4474226E-451.99393525
CCTCGAT203.451177E-451.98247518
ATATCGA150.0072269651.98247514
Produced by FastQC (version 0.11.5)