FastQCFastQC Report
Tue 31 May 2016
SRR212855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461296
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60901.3201935416738926No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43850.9505827061149457No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGTGG35850.7771582671430058No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT28150.6102372446325136No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22170.48060247650098853No Hit
CGTATTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13020.2822482744268322No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG11860.2571017307759009No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGTGG11740.2545003641913218No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGGGG11400.2471298255350144No Hit
CGTATTGGGGGAGGGGTGTTTGAACACACCCCTCCCACAGGACTCACTTTGTCCCAAC9440.20464083798688912No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA8930.19358503000242794No Hit
CGTATTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7880.17082307238736083No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGGGG7740.1677881447053519No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6510.1411241372134161No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG5610.12161388782907287No Hit
CGTATTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.10839027435746244No Hit
CGTATTGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTTTG4960.10752315216260276No Hit
CGTATTGGGGTGTGGGCTGTTCTCTCCTACTTTTGTGTGTGTGTGTGTGTGTGTGTGT4870.10557212722416842No Hit
CGTATTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC4810.10427144393187887No Hit
CGTATTGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4740.10275398009087441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACG150.007188861752.05213551
TCTGACG353.8658982E-852.01823445
CACGGTA150.00720740652.01823443
CGTGAAA150.00720740652.01823443
TTAACGT150.00720740652.01823444
ATAGCGC150.00721359652.00694333
GTTCGAC150.00721359652.00694337
ATTGCGA150.00721359652.00694334
AATAGCG150.00721978951.99566332
TCGTTAT203.446917E-451.99565530
GCGAATA150.007232187351.97310617
TAACGTT150.007232187351.97310615
CGTTCGT150.00724149751.956220
TTGGGAT62300.050.491285
TTGGGAC32450.050.4296725
ATTGGGC41250.049.9355164
ATTGGGG199800.049.6104554
CGTATTG483050.049.6060451
TATTGGG483200.049.590653
GTATTGG483400.049.570132
Produced by FastQC (version 0.11.5)