FastQCFastQC Report
Tue 31 May 2016
SRR212854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467964
Sequences flagged as poor quality0
Sequence length58
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55841.1932541819456197No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGTGG49971.0678171825183134No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39700.8483558564334008No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT26960.5761126924293322No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20450.43699942730637403No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGTGG13780.29446709575950286No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG12810.2737390055645306No Hit
CGTATTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11500.24574539921874333No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGGGG11080.23677034985597184No Hit
CGTATTGGGGGAGGGGTGTTTGAACACACCCCTCCCACAGGACTCACTTTGTCCCAAC8540.18249267037635375No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA7890.16860271302920737No Hit
CGTATTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.14445555641032215No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGGGG6430.13740373191100172No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6260.1337709738355942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT150.00700983152.3852310
GATATCG458.54925E-1152.385239
GGACGAC150.00700983152.385238
GCGTAGA150.007185044752.05922738
CACGGTA203.4462428E-451.99785243
CGAACCG150.007227821351.98114440
TCACCGT150.007227821351.98114450
TAACACG150.007227821351.98114450
TCTACGG150.00723088451.9755834
TACGACA150.00723088451.9755833
CGCGATT203.4645153E-451.9421930
GTAACGA203.4645153E-451.9421930
GTGCGAT401.9317667E-951.87554631
TTGGGAT63550.050.73665
ATTGGGG193900.050.0455934
ATTGGGC43050.050.003044
TTGGGAC33900.049.9900365
TTGGGGC38650.049.7422525
TTGGGGG63450.049.66075
TTGGGAG57100.049.587075
Produced by FastQC (version 0.11.5)