FastQCFastQC Report
Tue 31 May 2016
SRR212853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495236
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGT77661.5681412498283647No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58961.1905434984532626No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT45790.9246096810409582No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44820.9050230597129449No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGG39150.7905321907131146No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22170.4476653555072733No Hit
CGTATTGGGGTGTGGGCTGTTCTCTCCTACTTTTGTGTGTGTGTGTGTGTGTGTGT14950.30187627716886495No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14700.29682817888844915No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGT13390.27037614389907033No Hit
CGTATTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13340.2693665242429872No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA12440.25119337043349027No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGG11620.23463560807372644No Hit
CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT9880.19950084404203247No Hit
CGTATTGGGGGAGGGGTGTTTGAACACACCCCTCCCACAGGACTCACTTTGTCCCA8010.16174106890452228No Hit
CGTATTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7280.14700062192570815No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5680.11469279293104702No Hit
CGTATTGGGACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCATAAAAATCCTT5540.11186585789401417No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.10944277071941458No Hit
CGTATTGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC5100.10298120492048235No Hit
CGTATTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5010.10116388953953268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA355.1200914E-850.19688843
ACGGTAC355.1200914E-850.19688844
TACGTAC402.6157068E-950.13064240
ACTTACG150.00835507350.110338
CGTGAGT252.0661431E-550.0798135
CGCTCCG150.00837519850.07980735
CTATGCG252.0711064E-550.05950533
CGCACGT301.0402837E-650.04428531
TCATCGG301.0490403E-649.98350527
AGCACGC150.00843916649.98350527
ACGCGGA252.094817E-549.96327624
CATAACG150.00852048549.8623811
TTGGGAT69150.048.6726045
TTGGGAC35750.047.979465
TTGGGGC37350.047.7263765
ATTGGGG202250.047.6065564
ATTGGGC42700.047.5852934
GTGGTAT44600.047.17920315
TTGGGAG57550.047.176485
TGGGATT25700.047.0491336
Produced by FastQC (version 0.11.5)