FastQCFastQC Report
Tue 31 May 2016
SRR212852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448893
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53611.1942712405851728No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGT49081.0933563232217922No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40980.9129124312475355No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT38820.8647940600544005No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAGCAGG30880.6879144918722279No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20020.4459860144845208No Hit
CGTATTGGGGTGTGGGCTGTTCTCTCCTACTTTTGTGTGTGTGTGTGTGTGTGTGT16920.3769272410128917No Hit
CGTATTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13510.3009625901940997No Hit
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA12460.2775717153085479No Hit
CGTATTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT12420.27668063436052687No Hit
CGTATTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12270.27333908080544805No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGG9260.20628523946686628No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGGAGCAGT7600.1693053801239939No Hit
CGTATTGGGGGAGGGGTGTTTGAACACACCCCTCCCACAGGACTCACTTTGTCCCA7200.1603945706437837No Hit
CGTATTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6840.15237484211159452No Hit
CGTATTGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTC5760.12831565651502697No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5620.1251968731969534No Hit
CGTATTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.10492478162947518No Hit
CGTATTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.10447924115546466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG150.00826783450.2427648
TCCCGAT150.00826783450.2427648
CACGGTA204.089177E-450.22583843
ATCATCG150.00829355150.203341
CGTGAGT507.2759576E-1250.13018435
TAGGGCG150.00834886250.11895434
CGTTTAT150.00834886250.11895434
CCTACTA150.00838959750.05727832
CGAAAAC150.00844165549.97900427
TGAATCG150.00846403849.9455326
TAGGCGA204.203684E-449.94552626
ATAGGCG204.2059997E-449.93995325
CGATTCG150.00847898649.9232424
CGGTGAC301.0657459E-649.86760320
TTCGCAT150.00852394549.8564919
GGTACAT150.00853145649.8453838
AGATCGT150.00853145649.84538311
ATCGTAG150.00853145649.84538310
TCAAACG150.00853145649.84538318
GATCGTA204.2455245E-449.845389
Produced by FastQC (version 0.11.5)