FastQCFastQC Report
Tue 31 May 2016
SRR212851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201573
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40201.994314714768347No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26591.3191250812360782No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12780.6340134839487431No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8720.43259761972089517No Hit
TGATCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8450.41920296865155554No Hit
TGATCAGGGGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAG6220.3085730727825651No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.21629880986044758No Hit
TGATCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.1994314714768347No Hit
TGATCAGGGGAATAAATGCAGATGTAAACTGCAGTTCCTCCATACAACCAAACCCGGT3820.18950950772176828No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3730.1850446240319884No Hit
TGATCAGGGACAGCTACTTGCCCAGAGGCTTTGTAGCCTAGCTAGAGGGCCATGCCCA3620.1795875439667019No Hit
TGATCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.17363436571366206No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAGCAGTGG2930.1453567690117228No Hit
TGATCAGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT2650.13146601975462985No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2580.1279933324403566No Hit
TGATCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2540.12600893968934332No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2430.1205518596240568No Hit
TGATCAGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA2340.11608697593427691No Hit
TGATCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.11459868137101695No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2180.10814940493022379No Hit
TGATCAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCA2170.10765330674247046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTT150.007168439752.07885452
CGCTAAC203.4151785E-452.07885450
TAGGCCG150.007168439752.07885450
CGCTTTC150.007168439752.07885451
CCCGAGT150.007168439752.07885449
CGAAAGG150.007175495452.06590748
GCACGAT150.007175495452.06590741
CCGTCCG203.419376E-452.06590748
ATCAATT150.007175495452.06590743
ACTCATA150.007175495452.06590746
CGCACGA150.007182556752.0529640
CCGCACG150.007182556752.0529639
ATACCCC150.00718962352.0400237
TGGCGTA150.007196694652.0270934
TATCCGA150.007196694652.0270935
TCACGGG203.4319935E-452.02708434
CACGTCC203.4319935E-452.02708432
CAATCGG401.873559E-952.02708430
GTGCGAT251.6452037E-552.02708431
CCGCAGT150.007203771352.0141628
Produced by FastQC (version 0.11.5)