##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212851_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201573
Sequences flagged as poor quality	0
Sequence length	58
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.55141313568782	39.0	37.0	40.0	33.0	40.0
2	36.10524226954999	38.0	35.0	40.0	28.0	40.0
3	35.72363362156638	38.0	34.0	39.0	28.0	40.0
4	36.261810857604935	39.0	35.0	40.0	29.0	40.0
5	36.68575156394954	39.0	36.0	40.0	30.0	40.0
6	36.27396030222302	38.0	35.0	40.0	29.0	40.0
7	36.71214398753801	39.0	36.0	40.0	30.0	40.0
8	37.29838321600611	39.0	37.0	40.0	33.0	40.0
9	37.19666324358917	39.0	37.0	40.0	32.0	40.0
10	35.626904396918235	38.0	34.0	39.0	28.0	40.0
11	36.52218303046539	38.0	36.0	40.0	31.0	40.0
12	36.423886135543945	38.0	35.0	40.0	30.0	40.0
13	36.3937779365292	38.0	35.0	40.0	30.0	40.0
14	36.3375352849836	38.0	35.0	39.0	30.0	40.0
15	36.34490234307174	38.0	35.0	39.0	30.0	40.0
16	36.088801575607846	38.0	35.0	39.0	30.0	40.0
17	35.92313950777138	38.0	35.0	39.0	29.0	40.0
18	35.82579512137042	38.0	35.0	39.0	29.0	40.0
19	35.83155978231211	38.0	35.0	39.0	29.0	40.0
20	35.73717214111017	38.0	35.0	39.0	29.0	40.0
21	35.658996988684	38.0	35.0	39.0	28.0	40.0
22	35.57507701924365	38.0	35.0	39.0	28.0	40.0
23	35.333556577517825	38.0	34.0	39.0	28.0	40.0
24	35.26341325475138	38.0	34.0	39.0	28.0	40.0
25	35.272561305333554	38.0	34.0	39.0	28.0	40.0
26	34.877701874755054	38.0	34.0	39.0	26.0	40.0
27	34.811274327414885	38.0	33.0	39.0	26.0	40.0
28	34.56533365083617	38.0	33.0	39.0	26.0	40.0
29	34.62916164367252	38.0	33.0	39.0	26.0	40.0
30	34.39737960937229	38.0	33.0	39.0	26.0	40.0
31	34.080209154995956	38.0	33.0	39.0	24.0	40.0
32	33.90583560298254	38.0	33.0	39.0	23.0	40.0
33	33.77323847935983	37.0	33.0	39.0	23.0	40.0
34	33.58846670933111	37.0	32.0	39.0	23.0	40.0
35	33.40163613182321	37.0	32.0	39.0	23.0	40.0
36	34.174189995683946	38.0	33.0	39.0	25.0	40.0
37	34.062895328243364	38.0	33.0	39.0	24.0	40.0
38	33.91334156856325	38.0	33.0	39.0	24.0	40.0
39	33.84476095508823	38.0	33.0	39.0	23.0	40.0
40	33.66769855089719	38.0	33.0	39.0	23.0	40.0
41	33.75805787481458	38.0	33.0	39.0	23.0	40.0
42	33.64947190347914	38.0	33.0	39.0	23.0	40.0
43	33.476279065152575	38.0	33.0	39.0	22.0	40.0
44	33.3572551879468	38.0	33.0	39.0	22.0	40.0
45	33.17120844557555	37.0	33.0	39.0	21.0	40.0
46	32.881759957930875	37.0	33.0	39.0	18.0	40.0
47	32.71943166991611	37.0	32.0	39.0	18.0	40.0
48	32.616853447634355	37.0	32.0	39.0	17.0	40.0
49	32.48286724908594	36.0	32.0	39.0	16.0	40.0
50	32.16301290351387	36.0	31.0	39.0	11.0	40.0
51	31.953669390245718	36.0	32.0	39.0	2.0	40.0
52	31.79024472523602	36.0	31.0	39.0	2.0	40.0
53	31.578385994155962	36.0	31.0	39.0	2.0	40.0
54	31.4164545846914	36.0	31.0	39.0	2.0	40.0
55	31.105718523810232	36.0	30.0	39.0	2.0	40.0
56	30.842717030554688	36.0	30.0	39.0	2.0	40.0
57	30.611708909427353	36.0	30.0	39.0	2.0	40.0
58	30.386222361129715	36.0	30.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-4.036428853440732
1	2	-8.171171479680144
1	3	-8.777358668813932
1	4	-9.126835635625294
1	5	-6.301440678405978
1	6	-7.165539883603074
1	7	-10.937079254280462
1	8	-5.727128788459218
1	9	-6.417860448139599
1	10	-5.788062856673118
1	11	-3.3864431181801145
1	12	-3.639092641130617
1	13	-3.08463244813926
1	14	-1.5822930540764446
1	15	-8.655735928595046
1	16	-6.885411701873693
1	17	-7.820412893223377
1	18	-7.866849197152849
1	19	-6.406125028826441
1	20	-10.341190540704208
1	21	-9.826136298870182
1	22	-8.185922803175188
1	23	-7.373273807286292
1	24	-7.3300362051424415
1	25	-9.6753952399628
1	26	-7.596215802282817
1	27	-5.760645541254995
1	28	-5.515045834089072
1	29	-15.246613505567282
1	30	-11.286228779153952
1	31	-10.736473647939597
1	32	-16.076439724325823
1	33	-14.268970872106902
1	34	-13.696164974158396
1	35	-15.3334181258661
1	36	-14.526141031500963
1	37	-16.68144797788645
1	38	-17.10717616458902
1	39	-15.93017679943833
1	40	-15.335876005247194
1	41	-12.2289471504055
1	42	-14.450341053624776
1	43	-13.169515780893907
1	44	-12.771128939311055
1	45	-13.73857530818568
1	46	-10.679497383257555
1	47	-10.6443796595163
1	48	-13.534196881460819
1	49	-11.491395191536583
1	50	-13.622470226696585
1	51	-13.222237140107282
1	52	-13.877696236403548
1	53	-15.725218881219343
1	54	-12.450418962989485
1	55	-15.855199370727433
1	56	-14.08096688481768
1	57	-12.294998979438375
1	58	-13.950744731067497
2	1	-3.4890604323881007
2	2	-7.923803058627513
2	3	-7.851042879340248
2	4	-9.563677740888451
2	5	-8.290914362616505
2	6	-6.376066199392547
2	7	-6.8528687279646725
2	8	-3.548181420038162
2	9	-3.496807816560654
2	10	-7.5406944356204875
2	11	-3.9759168023906426
2	12	-3.933829483235879
2	13	-4.774106132349786
2	14	-5.192819369865916
2	15	-7.113630665437153
2	16	-7.538043280821064
2	17	-6.641465524802324
2	18	-7.324743933994956
2	19	-7.311388186721178
2	20	-6.8306642249147345
2	21	-7.347188930449132
2	22	-6.049080697912032
2	23	-4.731168544128398
2	24	-5.430036205142443
2	25	-5.1648689241733265
2	26	-8.112005275967025
2	27	-9.550119225465522
2	28	-8.888730044615386
2	29	-9.45187666346202
2	30	-9.870439305469741
2	31	-9.46805259530802
2	32	-10.097492355904773
2	33	-8.521602451054271
2	34	-8.669849184684711
2	35	-9.61236549428715
2	36	-7.973509452553593
2	37	-9.2025006094654
2	38	-7.817702480378493
2	39	-7.635439957333066
2	40	-8.862191794720879
2	41	-7.58157872935287
2	42	-7.771393685203723
2	43	-9.274778938788643
2	44	-7.702707886679477
2	45	-10.422785834501472
2	46	-10.842655277994396
2	47	-10.607537554253142
2	48	-10.81314424988187
2	49	-9.98086887574711
2	50	-9.611943910907112
2	51	-9.306447666423072
2	52	-11.061906762719339
2	53	-12.177850460166713
2	54	-11.103050541936856
2	55	-12.449936212832696
2	56	-10.854651095343996
2	57	-11.147630558385746
2	58	-10.850744731067497
3	1	-4.276428853440734
3	2	-7.391171479680143
3	3	-9.057358668813933
3	4	-8.226835635625292
3	5	-7.421440678405979
3	6	-7.405539883603073
3	7	-6.2570792542804625
3	8	-3.0471287884592186
3	9	-3.9978604481395976
3	10	-6.848062856673117
3	11	-4.3864431181801145
3	12	-5.119092641130614
3	13	-3.464632448139259
3	14	-4.662293054076443
3	15	-5.355735928595045
3	16	-5.205411701873693
3	17	-7.380412893223376
3	18	-4.846849197152849
3	19	-5.526125028826442
3	20	-6.161190540704208
3	21	-4.246136298870184
3	22	-6.705922803175191
3	23	-5.253273807286291
3	24	-4.910036205142443
3	25	-5.775395239962798
3	26	-5.476215802282816
3	27	-5.920645541254995
3	28	-6.895045834089071
3	29	-7.946613505567285
3	30	-6.6662287791539505
3	31	-9.036473647939598
3	32	-9.596439724325826
3	33	-9.588970872106902
3	34	-8.076164974158395
3	35	-8.3134181258661
3	36	-8.066141031500962
3	37	-7.021447977886449
3	38	-9.527176164589022
3	39	-8.970176799438331
3	40	-8.175876005247193
3	41	-7.2089471504055
3	42	-6.510341053624774
3	43	-9.129515780893907
3	44	-7.511128939311057
3	45	-8.818575308185679
3	46	-6.099497383257553
3	47	-7.904379659516298
3	48	-7.954196881460817
3	49	-6.911395191536581
3	50	-6.502470226696584
3	51	-7.622237140107281
3	52	-9.537696236403548
3	53	-7.805218881219343
3	54	-10.050418962989486
3	55	-7.995199370727434
3	56	-7.5809668848176806
3	57	-7.6949989794383775
3	58	-7.290744731067498
4	1	-3.091601267233834
4	2	-5.733240445197385
4	3	-7.446324186055314
4	4	-6.757870118383913
4	5	-6.191095850819771
4	6	-6.544850228430661
4	7	-4.778458564625293
4	8	-2.6098874091488753
4	9	-3.262688034346496
4	10	-6.419097339431737
4	11	-2.255408635421496
4	12	-1.728747813544409
4	13	-1.3294600343461553
4	14	-1.1891896058005784
4	15	-1.0936669630778084
4	16	-3.899204805321972
4	17	-3.2893784104647565
4	18	-3.7875388523252624
4	19	-3.999228477102303
4	20	-3.110156057945588
4	21	-1.891653540249493
4	22	-2.82040556179588
4	23	-4.5732738072862915
4	24	-2.9817603430734785
4	25	-4.8236711020317635
4	26	-3.48587097469661
4	27	-4.139955886082582
4	28	-6.311597558227003
4	29	-4.9673031607396965
4	30	-3.7379529170849857
4	31	-3.284749510008563
4	32	-3.090232827774102
4	33	-4.813798458313798
4	34	-3.678923594848051
4	35	-2.8265215741419603
4	36	-2.8537272383975143
4	37	-2.4711031503002445
4	38	-3.8485554749338498
4	39	-4.081900937369365
4	40	-5.439324281109261
4	41	-5.804809219371016
4	42	-6.798616915693739
4	43	-6.100550263652526
4	44	-4.7952668703455394
4	45	-5.048920135771887
4	46	-6.407083590154105
4	47	-7.10300034917147
4	48	-5.234196881460818
4	49	-6.156912432915892
4	50	-5.408677123248307
4	51	-6.001547484934868
4	52	-7.27424796054148
4	53	-6.952805088115895
4	54	-5.222832756092934
4	55	-5.6241648879688135
4	56	-4.139587574472852
4	57	-3.853619669093547
4	58	-4.023158524170945
5	1	-3.5743598879234924
5	2	-7.0435852727835915
5	3	-6.032531082607036
5	4	-7.8958011528666745
5	5	-5.570406195647358
5	6	-6.372436435327213
5	7	-7.2956999439356345
5	8	-3.058163271217836
5	9	-4.055791482622357
5	10	-6.07426975322484
5	11	-4.462305187145631
5	12	-6.073575399751306
5	13	-6.088080724001326
5	14	-5.085741329938511
5	15	-4.852287652732979
5	16	-4.933687563942659
5	17	-5.323861169085443
5	18	-4.270297473014917
5	19	-4.3440560633092
5	20	-5.730845713118001
5	21	-5.23648112645639
5	22	-5.716957285933809
5	23	-4.918101393493188
5	24	-5.705898274107959
5	25	-6.13401592961797
5	26	-5.003112354006955
5	27	-4.933059334358443
5	28	-5.484011351330452
5	29	-4.932820402119006
5	30	-4.289677055016018
5	31	-3.6985426134568407
5	32	-5.9523017932913405
5	33	-6.331039837624143
5	34	-6.368578767261845
5	35	-7.895487091383341
5	36	-9.543382410811308
5	37	-9.126275564093348
5	38	-9.400279612864882
5	39	-9.116383695990052
5	40	-8.68070359145409
5	41	-9.425498874543432
5	42	-9.453789329486842
5	43	-9.652274401583561
5	44	-7.691818594483468
5	45	-8.94547185990982
5	46	-7.269152555671347
5	47	-7.41334517675768
5	48	-7.06178308835737
5	49	-7.156912432915892
5	50	-7.443159881868997
5	51	-4.622237140107281
5	52	-5.067351408817341
5	53	-5.5734947432883075
5	54	-5.084901721610176
5	55	-7.96899247417571
5	56	-7.001656539990094
5	57	-7.026033462196995
5	58	-7.643848179343358
6	1	-2.230546500499557
6	2	-3.3123479502683786
6	3	-5.259711609990404
6	4	-5.768012106213529
6	5	-4.883793619582448
6	6	-3.194951648308958
6	7	-4.595902783692228
6	8	-2.568305259047449
6	9	-2.4766839775513674
6	10	-4.576298150790766
6	11	-2.451149000533057
6	12	-2.2155632293659124
6	13	-2.6905148010804325
6	14	-2.717587171723501
6	15	-3.691030046242105
6	16	-3.191294054814872
6	17	-3.9557070108704337
6	18	-4.313908020682263
6	19	-3.1473014994146773
6	20	-3.6294258348218555
6	21	-3.9261362988701833
6	22	-4.315334567881074
6	23	-4.3085679249333495
6	24	-4.077095028671856
6	25	-4.904807004668683
6	26	-5.3256275669887
6	27	-5.525351423607937
6	28	-5.709163481147893
6	29	-4.964260564390813
6	30	-5.568581720330421
6	31	-6.101179530292541
6	32	-6.794086783149353
6	33	-6.1748532250480785
6	34	-6.549106150628983
6	35	-5.504006361160215
6	36	-7.467317502089198
6	37	-5.89909503670998
6	38	-6.665999694000785
6	39	-5.971353270026565
6	40	-5.629993652306016
6	41	-5.205417738640794
6	42	-5.526811641860068
6	43	-6.1989275455997905
6	44	-5.8358348216639975
6	45	-5.532692955244503
6	46	-4.020673853845789
6	47	-4.020850247751593
6	48	-4.0283145285196404
6	49	-4.014924603301289
6	50	-6.2930584619907
6	51	-5.886943022460223
6	52	-5.848284471697664
6	53	-6.801689469454637
6	54	-7.315124845342428
6	55	-6.937552311903904
6	56	-5.410378649523565
6	57	-5.653822508850141
6	58	-5.733097672243968
7	1	-2.883797274493361
7	2	-6.818539900732773
7	3	-6.377358668813933
7	4	-6.221572477730557
7	5	-6.21196699419545
7	6	-5.7444872520241255
7	7	-6.8791845174383575
7	8	-2.9955498410907992
7	9	-3.2599657112974967
7	10	-5.619641804041539
7	11	-4.896969433969588
7	12	-3.1706715884990366
7	13	-1.4583166586655736
7	14	-2.982293054076443
7	15	-4.271525402279256
7	16	-5.827516965031588
7	17	-5.483570787960218
7	18	-5.403691302416007
7	19	-4.364019765668548
7	20	-5.093822119651577
7	21	-5.268241562028077
7	22	-4.891185961069926
7	23	-3.652221175707343
7	24	-5.1405625209319155
7	25	-5.928026818910169
7	26	-5.63832106544071
7	27	-5.129066593886574
7	28	-5.678203728825913
7	29	-5.872929295040969
7	30	-5.028334042311844
7	31	-5.783842068992229
7	32	-4.071176566431088
7	33	-6.600549819475322
7	34	-5.906691289947869
7	35	-4.8228918100766265
7	36	-4.499825242027278
7	37	-6.070921662096978
7	38	-7.1334919540627055
7	39	-5.3985978520699085
7	40	-5.3885075841945635
7	41	-5.423683992510764
7	42	-4.350341053624774
7	43	-5.06425262299917
7	44	-4.781655255100528
7	45	-3.7648910976593655
7	46	-5.842655277994396
7	47	-5.660169133200508
7	48	-5.102617934092397
7	49	-6.244026770483952
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120	2	-8.496171479680143
120	3	-10.252358668813933
120	4	-9.576835635625294
120	5	-9.501440678405977
120	6	-9.790539883603074
120	7	-7.787079254280464
120	8	-4.127128788459217
120	9	-4.667860448139599
120	10	-8.613062856673118
120	11	-2.561443118180115
120	12	-1.1640926411306154
120	13	-2.3596324481392585
120	14	-2.232293054076443
120	15	-3.205735928595047
120	16	-4.985411701873694
120	17	-2.9704128932233758
120	18	-3.0418491971528496
120	19	-4.331125028826442
120	20	-5.416190540704207
120	21	-5.051136298870183
120	22	-6.28592280317519
120	23	-2.9482738072862915
120	24	-2.930036205142443
120	25	-6.375395239962799
120	26	-1.2962158022828163
120	27	-0.6356455412549948
120	28	-8.16504583408907
120	29	-7.721613505567284
120	30	-5.56122877915395
120	31	-6.836473647939599
120	32	-6.051439724325824
120	33	-5.968970872106901
120	34	-6.321164974158396
120	35	-5.158418125866099
120	36	-10.776141031500963
120	37	-11.156447977886451
120	38	-11.60717616458902
120	39	-16.90517679943833
120	40	-12.585876005247194
120	41	-11.9039471504055
120	42	-11.475341053624774
120	43	-12.544515780893907
120	44	-12.946128939311055
120	45	-9.36357530818568
120	46	-7.329497383257554
120	47	-9.4693796595163
120	48	-8.359196881460818
120	49	-10.941395191536582
120	50	-7.697470226696584
120	51	-7.372237140107281
120	52	-10.752696236403548
120	53	-12.500218881219343
120	54	-9.425418962989486
120	55	-15.805199370727433
120	56	-12.380966884817681
120	57	-10.969998979438376
120	58	-11.100744731067497
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	323.0
3	21.0
4	72.0
5	87.0
6	155.0
7	285.0
8	332.0
9	403.0
10	461.0
11	469.0
12	594.0
13	716.0
14	719.0
15	724.0
16	811.0
17	858.0
18	959.0
19	1055.0
20	1145.0
21	1276.0
22	1459.0
23	1735.0
24	2038.0
25	2492.0
26	2919.0
27	3473.0
28	3986.0
29	4768.0
30	5553.0
31	6517.0
32	8276.0
33	9809.0
34	11532.0
35	13714.0
36	17267.0
37	24908.0
38	31879.0
39	37393.0
40	390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	36.061873365976595	53.98441259494079	2.9929603667157805	6.960753672366835
11	28.38376171411846	29.144280235944297	25.40171550753325	17.070242542403992
12	30.850361903627967	22.259925684491474	24.161966136337703	22.727746275542856
13	23.090890148978286	23.42426813114852	29.612596925183432	23.872244794689763
14	22.3457506709728	30.041721857590055	26.173148189489666	21.43937928194748
15	23.467428673483056	32.71866767870697	25.56294741855308	18.250956229256897
16	26.06450268637169	32.989041191032534	24.309307298100443	16.637148824495345
17	26.134452530844904	31.945250603999543	22.22619100772425	19.694105857431303
18	23.65705554342865	32.32939098684341	24.777746909292958	19.23580656043498
19	22.55803223709995	32.35219701442186	25.028154130843532	20.061616617634655
20	23.581648244795904	33.64157720318298	23.986962474946917	18.789812077074195
21	23.17488824833923	30.450529114966535	27.24309521092661	19.131487425767627
22	22.904966959696782	29.86029805726986	25.73522116166928	21.499513821364076
23	20.76927274801927	33.96786180277525	26.23891807686774	19.023947372337734
24	20.719044713329662	34.51305482381073	24.81284695867006	19.95505350418955
25	22.080338140524773	35.47002822798688	22.981252449484803	19.46838118200354
26	22.682028838806357	35.624261911141545	22.55599551440452	19.137713735647583
27	20.149331481159923	32.36970704239327	24.689306179148165	22.79165529729864
28	20.88386494908795	33.3781584327425	26.4097576467319	19.328218971437643
29	23.943179788437494	31.17421160219898	24.28801079643559	20.594597812927937
30	20.31914629606651	32.16577739173594	26.55813376349423	20.956942548703314
31	22.482293791535763	32.10940873423564	25.751552240139368	19.65674523408923
32	21.570699853122147	31.909709816998134	28.628279941248856	17.89131038863086
33	22.828465251970965	31.52601574787523	28.026653303630344	17.61886569652346
34	21.74920603414053	32.149662564509725	28.112842397776895	17.988289003572845
35	19.826414045674245	36.001965124011235	27.509751183540597	16.661869646773923
36	21.00500816494682	33.88179936566553	27.64368072824377	17.469511741143886
37	20.77000208484319	34.54734083215027	27.42611216456362	17.25654491844292
38	20.18250693834185	35.21897357224068	26.93417139566174	17.66434809375574
39	20.59150566884419	33.44072037011298	27.587216805988525	18.380557155054305
40	19.98173234913055	32.79787936400776	28.858420741726192	18.361967545135492
41	19.721987787320657	33.7154346423075	28.78766817256615	17.77490939780569
42	20.40073492898997	32.97596583573344	27.783295262687457	18.840003972589134
43	19.050480172405234	33.74911860804624	28.18319048990496	19.01721072964357
44	19.228878816012234	32.91017321927704	29.127548766017448	18.733399198693284
45	20.46466602129719	32.12947104525033	28.52086280934296	18.885000124109514
46	20.137423605284454	34.90450350261393	26.901862268581727	18.056210623519892
47	19.487421461742866	34.23597486775772	28.26830903717684	18.00829463332257
48	21.703070214646843	32.827259453499806	27.429303614529633	18.04036671732372
49	20.790859413810235	32.856433184302034	27.3397913561848	19.012916045702934
50	18.22467746021968	33.48303219684738	28.718335958031325	19.57395438490161
51	19.059089983460566	33.552700199168555	28.2367374104114	19.151472406959478
52	19.95103370529839	34.125435159388765	28.238057637202463	17.685473498110376
53	19.001584707325918	33.05878320309589	29.145201913571356	18.794430176006834
54	19.37142601636518	34.16964343380647	27.390563441159376	19.068367108668976
55	19.395731358859045	33.290928516411164	27.925559669043654	19.387780455686137
56	19.690448025289026	34.71574699046691	26.91283040249709	18.68097458174697
57	20.243029670493513	34.788032403956066	26.7750111823468	18.19392674320362
58	20.958033233424295	36.165580590805384	26.32429181392066	16.552094361849658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	701.0
9	3593.0
10	5784.0
11	6611.5
12	7439.0
13	5477.0
14	3515.0
15	2868.5
16	2049.5
17	1877.0
18	1616.0
19	1355.0
20	1497.0
21	1639.0
22	1855.0
23	2392.0
24	2713.0
25	3120.0
26	3527.0
27	4132.5
28	4808.5
29	4879.0
30	4793.5
31	4708.0
32	5618.0
33	6528.0
34	6051.5
35	5861.0
36	6147.0
37	6140.0
38	6133.0
39	6196.5
40	6260.0
41	6906.5
42	7636.0
43	7719.0
44	7967.5
45	8216.0
46	8059.5
47	8287.0
48	8671.0
49	8408.5
50	8146.0
51	8622.0
52	9098.0
53	9686.5
54	9637.5
55	9000.0
56	9560.0
57	10120.0
58	9489.0
59	8091.5
60	7325.0
61	6753.5
62	6182.0
63	5420.5
64	4659.0
65	3912.0
66	2571.0
67	1977.0
68	1669.0
69	1361.0
70	1217.0
71	1073.0
72	893.5
73	555.0
74	396.0
75	329.0
76	262.0
77	216.5
78	134.5
79	98.0
80	82.5
81	67.0
82	52.5
83	38.0
84	23.5
85	5.5
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	4.960981877533201E-4
19	0.0019843927510132804
20	4.960981877533201E-4
21	0.004960981877533201
22	4.960981877533201E-4
23	0.002976589126519921
24	0.0
25	0.0
26	0.019347829322379485
27	0.003968785502026561
28	0.024308811199912687
29	0.012402454693833002
30	0.04861762239982537
31	0.04464883689779881
32	0.022324418448899406
33	0.010914160130573043
34	0.02629320395092597
35	0.02926979307744589
36	0.05060201515083865
37	0.0590356843426451
38	0.07739131728951794
39	0.06002788071815174
40	0.06151617528141169
41	0.07094204084872478
42	0.09574695023639078
43	0.09277036110987086
44	0.07540692453850466
45	0.06846154990995819
46	0.07540692453850466
47	0.1180713686852902
48	0.1086455031179771
49	0.1354348052566564
50	0.13890749257092966
51	0.11707917230978356
52	0.10418061942819724
53	0.13593090344440972
54	0.14486067082396947
55	0.16768118746062222
56	0.18802121315850834
57	0.18008364215445521
58	0.19347829322379487
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	201573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.310016718508926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.8989634218992	20.28545489723326
2	12.90063147861313	8.594405004638519
3	7.132431788395091	7.1274426634519505
4	4.716728225902537	6.2845718424590595
5	3.3435601096151553	5.5687021575310185
6	2.3605981174788515	4.7178937655340745
7	1.806564994638389	4.212369712213442
8	1.3404027165495056	3.571906951823905
9	1.0321100917431194	3.0941643970174577
>10	4.208864529965448	22.34872726009932
>50	0.15489098057905398	3.5932391738972975
>100	0.09531752651018706	5.493295232992514
>500	0.004468009055165019	1.1603736611550157
>1k	0.004468009055165019	3.9474532799531685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4020	1.994314714768347	No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2659	1.3191250812360782	No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1278	0.6340134839487431	No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	872	0.43259761972089517	No Hit
TGATCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	845	0.41920296865155554	No Hit
TGATCAGGGGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAG	622	0.3085730727825651	No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	436	0.21629880986044758	No Hit
TGATCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402	0.1994314714768347	No Hit
TGATCAGGGGAATAAATGCAGATGTAAACTGCAGTTCCTCCATACAACCAAACCCGGT	382	0.18950950772176828	No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG	373	0.1850446240319884	No Hit
TGATCAGGGACAGCTACTTGCCCAGAGGCTTTGTAGCCTAGCTAGAGGGCCATGCCCA	362	0.1795875439667019	No Hit
TGATCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	350	0.17363436571366206	No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAGCAGTGG	293	0.1453567690117228	No Hit
TGATCAGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT	265	0.13146601975462985	No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	258	0.1279933324403566	No Hit
TGATCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	254	0.12600893968934332	No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	243	0.1205518596240568	No Hit
TGATCAGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA	234	0.11608697593427691	No Hit
TGATCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	231	0.11459868137101695	No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	218	0.10814940493022379	No Hit
TGATCAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCA	217	0.10765330674247046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.002976589126519921	0.0	0.0	0.0	0.0
11	0.006449276440793162	0.0	0.0	0.0	0.0
12	0.006945374628546482	0.0	0.0	0.0	0.0
13	0.006945374628546482	0.0	0.0	0.0	0.0
14	0.016371240195859563	0.0	0.0	0.0	0.0
15	0.017859534759119524	0.0	0.0	0.0	0.0
16	0.018355632946872844	0.0	0.0	0.0	0.0
17	0.018355632946872844	0.0	0.0	0.0	0.0
18	0.018851731134626164	0.0	0.0	0.0	0.0
19	0.019843927510132805	0.0	0.0	0.0	0.0
20	0.024308811199912687	0.0	0.0	0.0	0.0
21	0.025797105763172647	0.0	0.0	0.0	0.0
22	0.026293203950925968	0.0	0.0	0.0	0.0
23	0.03224638220396581	0.0	0.0	0.0	0.0
24	0.03671126589374569	0.0	0.0	0.0	0.0
25	0.04018395320801893	0.0	0.0	0.0	0.0
26	0.04117614958352557	0.0	0.0	0.0	0.0
27	0.04316054233453885	0.0	0.0	0.0	0.0
28	0.05953178253039842	0.0	0.0	0.0	0.0
29	0.06250837165691833	0.0	0.0	0.0	0.0
30	0.07292643359973806	0.0	0.0	0.0	0.0
31	0.0739186299752447	0.0	0.0	0.0	0.0
32	0.07689521910176463	0.0	0.0	0.0	0.0
33	0.07937571004053122	0.0	0.0	0.0	0.0
34	0.08235229916705114	0.0	0.0	0.0	0.0
35	0.10070793211392398	0.0	0.0	0.0	0.0
36	0.10418061942819723	0.0	0.0	0.0	0.0
37	0.10566891399145718	0.0	0.0	0.0	0.0
38	0.10814940493022379	0.0	0.0	0.0	0.0
39	0.11013379768123707	0.0	0.0	0.0	0.0
40	0.1136064849955103	0.0	0.0	0.0	0.0
41	0.11559087774652359	0.0	0.0	0.0	0.0
42	0.1180713686852902	0.0	0.0	0.0	0.0
43	0.12005576143630348	0.0	0.0	0.0	0.0
44	0.12104795781181012	0.0	0.0	0.0	0.0
45	0.12402454693833004	0.0	0.0	0.0	0.0
46	0.12551284150159	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCTT	15	0.0071684397	52.078854	52
CGCTAAC	20	3.4151785E-4	52.078854	50
TAGGCCG	15	0.0071684397	52.078854	50
CGCTTTC	15	0.0071684397	52.078854	51
CCCGAGT	15	0.0071684397	52.078854	49
CGAAAGG	15	0.0071754954	52.065907	48
GCACGAT	15	0.0071754954	52.065907	41
CCGTCCG	20	3.419376E-4	52.065907	48
ATCAATT	15	0.0071754954	52.065907	43
ACTCATA	15	0.0071754954	52.065907	46
CGCACGA	15	0.0071825567	52.05296	40
CCGCACG	15	0.0071825567	52.05296	39
ATACCCC	15	0.007189623	52.04002	37
TGGCGTA	15	0.0071966946	52.02709	34
TATCCGA	15	0.0071966946	52.02709	35
TCACGGG	20	3.4319935E-4	52.027084	34
CACGTCC	20	3.4319935E-4	52.027084	32
CAATCGG	40	1.873559E-9	52.027084	30
GTGCGAT	25	1.6452037E-5	52.027084	31
CCGCAGT	15	0.0072037713	52.01416	28
>>END_MODULE