FastQCFastQC Report
Tue 31 May 2016
SRR212850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214702
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43162.0102281301524907No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27001.257556985961938No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13320.6203947797412227No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9700.45178899125299254No Hit
TGATCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8540.3977606170412944No Hit
TGATCAGGGGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAG6790.3162522938770948No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4960.23101787593967452No Hit
TGATCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.21052435468696148No Hit
TGATCAGGGGAATAAATGCAGATGTAAACTGCAGTTCCTCCATACAACCAAACCCGGT4290.19981183221395235No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG4200.19561997559407923No Hit
TGATCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.18164712019450216No Hit
TGATCAGGGACAGCTACTTGCCCAGAGGCTTTGTAGCCTAGCTAGAGGGCCATGCCCA3440.16022207524848395No Hit
TGATCAGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT2790.12994755521606693No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2760.12855026967610922No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAGCAGTGG2650.12342688936293093No Hit
TGATCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.12202960382297323No Hit
TGATCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.11783774720310011No Hit
TGATCAGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA2410.11224860504326928No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2390.11131708134996413No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2340.1089882721167013No Hit
TGATCAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCA2340.1089882721167013No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG2320.10805674842339615No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10339912995687044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG203.4096136E-452.0976251
TTTCGAT150.0071787352.06114649
CTAGAGG550.052.03685842
CGACGCT150.007191992452.03685838
GCACGAT150.007191992452.03685840
ACGCTAT150.007191992452.03685840
CCGCACG150.007191992452.03685838
GTACGTA150.007191992452.03685839
TGCGGAT251.6437409E-552.03685438
CGGATTG251.6437409E-552.03685440
CCGGGAT150.007205272652.01259226
CCGGCGA307.9511665E-752.01259234
CATTCGA150.007205272652.01259225
GCCTCGC150.007205272652.01259227
CGGGATT150.007205272652.01259227
TGTCGTT150.007205272652.01259224
TCGGTAA150.007205272652.01259227
CGAACGT150.007211919452.00046529
CGGTAGA150.007211919452.00046531
GGCGAGT150.007211919452.00046529
Produced by FastQC (version 0.11.5)