##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212850_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 214702 Sequences flagged as poor quality 0 Sequence length 58 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.53060986856201 39.0 37.0 40.0 33.0 40.0 2 36.1201758716733 38.0 35.0 40.0 29.0 40.0 3 35.750812754422405 38.0 35.0 39.0 28.0 40.0 4 36.25296923177241 38.0 35.0 40.0 29.0 40.0 5 36.68718968616967 39.0 36.0 40.0 30.0 40.0 6 36.27616882935418 38.0 35.0 40.0 29.0 40.0 7 36.7521634637777 39.0 36.0 40.0 31.0 40.0 8 37.29909828506488 39.0 37.0 40.0 33.0 40.0 9 37.20366368268577 39.0 37.0 40.0 32.0 40.0 10 35.571634172015166 38.0 34.0 39.0 28.0 40.0 11 36.48224981602407 38.0 35.0 39.0 31.0 40.0 12 36.38315898314874 38.0 35.0 40.0 30.0 40.0 13 36.292726663002675 38.0 35.0 39.0 30.0 40.0 14 36.29941034550214 38.0 35.0 39.0 30.0 40.0 15 36.295833294519845 38.0 35.0 39.0 30.0 40.0 16 35.98849568238768 38.0 35.0 39.0 29.0 40.0 17 35.84387197138359 38.0 35.0 39.0 29.0 40.0 18 35.7335888813332 38.0 35.0 39.0 29.0 40.0 19 35.739392273942485 38.0 35.0 39.0 29.0 40.0 20 35.64488919525668 38.0 35.0 39.0 29.0 40.0 21 35.57998062430718 38.0 35.0 39.0 28.0 40.0 22 35.523614125625286 38.0 35.0 39.0 28.0 40.0 23 35.26406833657814 38.0 34.0 39.0 28.0 40.0 24 35.16482380229341 38.0 34.0 39.0 28.0 40.0 25 35.187282838539 38.0 34.0 39.0 28.0 40.0 26 34.741003809931904 38.0 33.0 39.0 26.0 40.0 27 34.54377695596688 38.0 33.0 39.0 26.0 40.0 28 34.40813779098471 38.0 33.0 39.0 26.0 40.0 29 34.45129528369554 38.0 33.0 39.0 26.0 40.0 30 34.21167478644819 38.0 33.0 39.0 25.0 40.0 31 33.926651824389154 38.0 33.0 39.0 23.0 40.0 32 33.74444578997867 37.0 33.0 39.0 23.0 40.0 33 33.60383694609273 37.0 32.0 39.0 23.0 40.0 34 33.43081107767976 37.0 32.0 39.0 22.0 40.0 35 33.22756658065598 36.0 32.0 39.0 22.0 40.0 36 34.02229601959926 38.0 33.0 39.0 24.0 40.0 37 33.90690817970955 38.0 33.0 39.0 24.0 40.0 38 33.72347719164237 38.0 33.0 39.0 23.0 40.0 39 33.66451639947462 38.0 33.0 39.0 23.0 40.0 40 33.49635308474071 37.0 33.0 39.0 23.0 40.0 41 33.54621754804334 38.0 33.0 39.0 22.0 40.0 42 33.41980512524336 38.0 33.0 39.0 22.0 40.0 43 33.262736257696716 37.0 33.0 39.0 22.0 40.0 44 33.12241152853723 37.0 33.0 39.0 21.0 40.0 45 32.97608778679286 37.0 32.0 39.0 20.0 40.0 46 32.59095863103278 37.0 32.0 39.0 16.0 40.0 47 32.41697329321571 36.0 32.0 39.0 16.0 40.0 48 32.3093077847435 36.0 32.0 39.0 15.0 40.0 49 32.140580898175145 36.0 31.0 39.0 13.0 40.0 50 31.81672737096068 36.0 31.0 39.0 5.0 40.0 51 31.602933368110218 36.0 31.0 39.0 2.0 40.0 52 31.42490055984574 36.0 31.0 39.0 2.0 40.0 53 31.198507699043326 36.0 30.0 39.0 2.0 40.0 54 31.031657832716974 36.0 30.0 39.0 2.0 40.0 55 30.73958789391808 36.0 30.0 39.0 2.0 40.0 56 30.452319959758178 36.0 30.0 39.0 2.0 40.0 57 30.19863811236039 35.0 30.0 39.0 2.0 40.0 58 30.005863941649356 35.0 29.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -2.881557482677607 1 2 -7.042417706378984 1 3 -7.846522703875017 1 4 -8.128027670156253 1 5 -7.1228125145125425 1 6 -6.486912310442193 1 7 -7.532252711878385 1 8 -4.907350495292903 1 9 -4.356707902698808 1 10 -7.225994874230608 1 11 -6.447287407307623 1 12 -4.838183638230621 1 13 -4.354957263500857 1 14 -4.228009279666061 1 15 -4.5171770382889775 1 16 -4.777468639430147 1 17 -4.303503276644776 1 18 -6.604222801933229 1 19 -5.201562391735418 1 20 -5.935245725575989 1 21 -6.693293747264427 1 22 -7.114793147318114 1 23 -6.596717322842046 1 24 -9.060562340070561 1 25 -7.553583763570426 1 26 -8.836923871183814 1 27 -7.878305649819417 1 28 -9.912789572321156 1 29 -9.774429386677753 1 30 -7.881711056147136 1 31 -9.174561980420037 1 32 -8.992043178248117 1 33 -8.006923794314776 1 34 -8.364095923856912 1 35 -9.689468100464413 1 36 -9.916911517132604 1 37 -10.00422553611702 1 38 -10.75435255003162 1 39 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-8.871685094826592 120 18 -7.358768256478683 120 19 -8.628835119008144 120 20 -7.967063907394174 120 21 -10.638748292718972 120 22 -10.973884056409023 120 23 -9.8012627773875 120 24 -9.165107794616013 120 25 -9.421765581752243 120 26 -11.63237841663836 120 27 -10.719214740728507 120 28 -10.840062299593882 120 29 -11.038065750314114 120 30 -12.70443832887441 120 31 -10.120016525874583 120 32 -11.937497723702663 120 33 -11.434196521587502 120 34 -11.827732287493276 120 35 -10.157649918646229 120 36 -10.448729698950789 120 37 -12.149680081571567 120 38 -11.631625277304344 120 39 -9.509869411825736 120 40 -11.295884415935188 120 41 -7.619877433572281 120 42 -8.540499339797702 120 43 -8.489821068303389 120 44 -7.382889464474161 120 45 -8.808178884057426 120 46 -6.672032857346974 120 47 -7.276592431116008 120 48 -7.183157390154442 120 49 -7.058666847787077 120 50 -6.687531459793661 120 51 -9.533002657045682 120 52 -8.327746510268511 120 53 -8.279252005867654 120 54 -8.016944697263334 120 55 -10.988999096795503 120 56 -8.666842071858689 120 57 -8.240510192713494 120 58 -8.69994655383573 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 365.0 3 17.0 4 91.0 5 85.0 6 219.0 7 308.0 8 384.0 9 466.0 10 458.0 11 552.0 12 683.0 13 789.0 14 791.0 15 846.0 16 944.0 17 975.0 18 981.0 19 1169.0 20 1251.0 21 1395.0 22 1632.0 23 1919.0 24 2218.0 25 2610.0 26 3383.0 27 3813.0 28 4382.0 29 5209.0 30 5911.0 31 7229.0 32 9040.0 33 11019.0 34 12766.0 35 14770.0 36 18690.0 37 26334.0 38 32890.0 39 37698.0 40 420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 35.97544503544447 53.9603729820868 3.0255889558550924 7.038593026613632 11 28.35371817682183 29.162280742610687 25.5032556753081 16.98074540525938 12 30.8129407271474 22.320704977131093 24.098517945803952 22.767836349917562 13 23.00723793909698 23.45110897895688 29.779880951272 23.761772130674146 14 22.55638047153729 30.049091298637183 26.00860727892614 21.385920950899386 15 23.383573511192257 32.86182709057205 25.471118107889072 18.28348129034662 16 25.77805516483312 33.25912194576669 24.171642555728404 16.791180333671786 17 25.83487811012473 31.976879581932167 22.400350252908684 19.78789205503442 18 23.682861828618286 32.26173170822617 24.917999179991803 19.13740728316374 19 22.491953851671408 32.35413299549602 25.08954396620385 20.06436918662872 20 23.495356354388875 33.61962161507578 24.013749289713186 18.87127274082216 21 23.168391538038772 30.423098061499193 27.323918733872997 19.08459166658904 22 22.959837168900005 29.843363561417974 25.933740411083324 21.2630588585987 23 20.802884010787096 33.97236156329034 26.099086636764618 19.125667789157948 24 20.49431942574728 34.61400509598051 24.97077031288575 19.92090516538646 25 21.677487866903896 35.6629188363406 23.404532794291622 19.25506050246388 26 22.614483815521634 35.48893474504032 22.643363874772337 19.253217564665714 27 20.05688308660683 32.352954889847304 24.912460659750554 22.677701363795315 28 21.109071767303625 33.146842070938106 26.35070295249364 19.393383209264627 29 23.829135006452397 31.148992075434776 24.36722276833342 20.654650149779407 30 20.17443269750704 32.308739348511125 26.588167291305403 20.92866066267644 31 22.347318554679312 32.1088695214716 25.894940818617563 19.64887110523153 32 21.361245323281786 31.99597441142074 28.780162794056668 17.862617471240803 33 22.687113820001677 31.610143428299832 28.056588475410294 17.646154276288197 34 21.749888168195035 32.081283083575634 28.313483187951988 17.855345560277343 35 19.80932499557328 35.778589601409095 27.716373260766243 16.69571214225138 36 20.880601049599644 33.887340486022424 27.807399397831823 17.42465906654611 37 20.635741955333046 34.59894369315825 27.529239561628 17.23607478988071 38 20.028064090550977 35.251201581285635 27.14804507037001 17.572689257793378 39 20.539290426965454 33.5571439895182 27.67185627625695 18.231709307259393 40 19.94937841730643 32.703127257811154 28.95778271874257 18.389711606139848 41 19.6527317996587 33.72203623749266 28.841258147840765 17.78397381500788 42 20.270509688368364 33.27334439864978 27.930288506368772 18.52585740661308 43 19.01625129367011 33.67504871658601 28.385219993846327 18.92347999589755 44 19.034475041263743 33.03011087591037 29.169503063308373 18.765911019517517 45 20.634965034965035 31.92027972027972 28.5995337995338 18.845221445221444 46 19.94255097901152 34.870902910221915 27.257508708283012 17.92903740248355 47 19.43331545439705 34.157319092371914 28.279657657237472 18.129707795993564 48 21.46691853821218 32.90421884838732 27.507171342615266 18.121691270785234 49 20.624413999636154 33.10056582562495 27.244060696809825 19.03095947792907 50 18.115066499349236 33.5025167589556 28.988211585021666 19.394205156673493 51 18.940037132754263 33.552429023259286 28.49379099297463 19.013742851011823 52 20.08340291350446 33.95543448346635 28.20632425448151 17.75483834854768 53 18.95281852741563 33.08440422604193 29.217529211465354 18.74524803507708 54 19.100987290270805 34.16696216942573 27.65065974879155 19.081390791511915 55 19.143921290418938 33.35449182286611 28.159771488313044 19.341815398401913 56 19.479227946326024 34.67938482225397 27.1526085291948 18.68877870222521 57 20.10719104375878 34.812345644430124 27.067605988879397 18.012857322931694 58 21.109389005079176 35.977647893636096 26.363161039737076 16.549802061547656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 745.0 9 3880.5 10 6271.0 11 7014.0 12 7757.0 13 5780.5 14 3804.0 15 3058.5 16 2223.0 17 2133.0 18 1809.5 19 1486.0 20 1571.5 21 1657.0 22 1920.5 23 2624.5 24 3065.0 25 3459.0 26 3853.0 27 4508.0 28 5132.5 29 5102.0 30 5155.5 31 5209.0 32 6007.0 33 6805.0 34 6412.0 35 6295.0 36 6571.0 37 6581.0 38 6591.0 39 6699.5 40 6808.0 41 7448.5 42 8020.0 43 7951.0 44 8381.0 45 8811.0 46 8723.5 47 9009.0 48 9382.0 49 9089.5 50 8797.0 51 9298.5 52 9800.0 53 10273.0 54 10152.5 55 9559.0 56 10022.5 57 10486.0 58 9870.0 59 8490.0 60 7726.0 61 7074.5 62 6423.0 63 5679.5 64 4936.0 65 4125.0 66 2707.5 67 2101.0 68 1752.0 69 1403.0 70 1307.5 71 1212.0 72 946.5 73 561.0 74 441.0 75 367.0 76 293.0 77 223.0 78 130.0 79 107.0 80 74.5 81 42.0 82 42.5 83 43.0 84 32.5 85 15.5 86 9.0 87 5.0 88 1.0 89 1.5 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.032603329265679874 19 0.0013972855399577088 20 0.0 21 0.0 22 4.657618466525696E-4 23 4.657618466525696E-4 24 0.010712522473009101 25 0.0 26 0.009780998779703962 27 0.10572793919013329 28 0.016767426479492507 29 0.02468537787258619 30 0.08290560870415739 31 0.010712522473009101 32 0.03493213849894272 33 0.04657618466525696 34 0.04378161358534154 35 0.04471313727864668 36 0.06706970591797003 37 0.08430289424411509 38 0.08989203640394594 39 0.10945403396335385 40 0.07871375208428426 41 0.10619370103678587 42 0.10153608257026017 43 0.09128932194390364 44 0.10619370103678587 45 0.09408389302381906 46 0.11690622350979497 47 0.13833126845581317 48 0.14298888692233885 49 0.15137260016208512 50 0.1578932660152211 51 0.1564959804752634 52 0.14950955277547484 53 0.14764650538886456 54 0.17605797803467133 55 0.20772978360704603 56 0.24219616025933619 57 0.23520973255954764 58 0.23008635224636942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 214702.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.562798669784165 #Duplication Level Percentage of deduplicated Percentage of total 1 61.94837635303914 20.791608834570706 2 12.618651124063279 8.47034494322363 3 6.878989730779905 6.926344421570363 4 4.378295864557313 5.877914504755428 5 3.330557868442964 5.5891421598308355 6 2.321676380793783 4.675317416698493 7 1.745767416042187 4.1014988216225285 8 1.2725506522342493 3.4168289070432505 9 1.015820149875104 3.0684390457471284 >10 4.249236747155148 23.129733304766606 >50 0.14571190674437967 3.471788804948254 >100 0.08603941160144324 5.427057037195741 >500 0.004163197335553706 1.1658019021713817 >1k 0.004163197335553706 3.8881798958556515 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4316 2.0102281301524907 No Hit TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2700 1.257556985961938 No Hit TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1332 0.6203947797412227 No Hit TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 970 0.45178899125299254 No Hit TGATCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 854 0.3977606170412944 No Hit TGATCAGGGGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAG 679 0.3162522938770948 No Hit TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 496 0.23101787593967452 No Hit TGATCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 452 0.21052435468696148 No Hit TGATCAGGGGAATAAATGCAGATGTAAACTGCAGTTCCTCCATACAACCAAACCCGGT 429 0.19981183221395235 No Hit TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG 420 0.19561997559407923 No Hit TGATCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 390 0.18164712019450216 No Hit TGATCAGGGACAGCTACTTGCCCAGAGGCTTTGTAGCCTAGCTAGAGGGCCATGCCCA 344 0.16022207524848395 No Hit TGATCAGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT 279 0.12994755521606693 No Hit TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 276 0.12855026967610922 No Hit TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAGCAGTGG 265 0.12342688936293093 No Hit TGATCAGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 262 0.12202960382297323 No Hit TGATCAGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 253 0.11783774720310011 No Hit TGATCAGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA 241 0.11224860504326928 No Hit TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 239 0.11131708134996413 No Hit TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 234 0.1089882721167013 No Hit TGATCAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCA 234 0.1089882721167013 No Hit TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGGAGGATTTGCTGAAGGAGGCAGAGG 232 0.10805674842339615 No Hit TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 222 0.10339912995687044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0018630473866102784 0.0 0.0 0.0 0.0 11 0.01117828431966167 0.0 0.0 0.0 0.0 12 0.013041331706271949 0.0 0.0 0.0 0.0 13 0.013507093552924518 0.0 0.0 0.0 0.0 14 0.018630473866102785 0.0 0.0 0.0 0.0 15 0.019096235712755354 0.0 0.0 0.0 0.0 16 0.020027759406060493 0.0 0.0 0.0 0.0 17 0.02189080679267077 0.0 0.0 0.0 0.0 18 0.02235656863932334 0.0 0.0 0.0 0.0 19 0.02282233048597591 0.0 0.0 0.0 0.0 20 0.031671805572374735 0.0 0.0 0.0 0.0 21 0.032603329265679874 0.0 0.0 0.0 0.0 22 0.03493213849894272 0.0 0.0 0.0 0.0 23 0.03726094773220557 0.0 0.0 0.0 0.0 24 0.041918566198731265 0.0 0.0 0.0 0.0 25 0.04704194651190953 0.0 0.0 0.0 0.0 26 0.051699564978435225 0.0 0.0 0.0 0.0 27 0.0535626123650455 0.0 0.0 0.0 0.0 28 0.07079580069119058 0.0 0.0 0.0 0.0 29 0.07265884807780086 0.0 0.0 0.0 0.0 30 0.07591918100436884 0.0 0.0 0.0 0.0 31 0.0782479902376317 0.0 0.0 0.0 0.0 32 0.08011103762424197 0.0 0.0 0.0 0.0 33 0.08243984685750481 0.0 0.0 0.0 0.0 34 0.08430289424411509 0.0 0.0 0.0 0.0 35 0.10153608257026017 0.0 0.0 0.0 0.0 36 0.10339912995687045 0.0 0.0 0.0 0.0 37 0.10526217734348073 0.0 0.0 0.0 0.0 38 0.10619370103678587 0.0 0.0 0.0 0.0 39 0.10991979581000642 0.0 0.0 0.0 0.0 40 0.11131708134996414 0.0 0.0 0.0 0.0 41 0.11318012873657442 0.0 0.0 0.0 0.0 42 0.11550893796983726 0.0 0.0 0.0 0.0 43 0.11970079458971039 0.0 0.0 0.0 0.0 44 0.12156384197632067 0.0 0.0 0.0 0.0 45 0.12435841305623609 0.0 0.0 0.0 0.0 46 0.12528993674954123 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGATGG 20 3.4096136E-4 52.09762 51 TTTCGAT 15 0.00717873 52.061146 49 CTAGAGG 55 0.0 52.036858 42 CGACGCT 15 0.0071919924 52.036858 38 GCACGAT 15 0.0071919924 52.036858 40 ACGCTAT 15 0.0071919924 52.036858 40 CCGCACG 15 0.0071919924 52.036858 38 GTACGTA 15 0.0071919924 52.036858 39 TGCGGAT 25 1.6437409E-5 52.036854 38 CGGATTG 25 1.6437409E-5 52.036854 40 CCGGGAT 15 0.0072052726 52.012592 26 CCGGCGA 30 7.9511665E-7 52.012592 34 CATTCGA 15 0.0072052726 52.012592 25 GCCTCGC 15 0.0072052726 52.012592 27 CGGGATT 15 0.0072052726 52.012592 27 TGTCGTT 15 0.0072052726 52.012592 24 TCGGTAA 15 0.0072052726 52.012592 27 CGAACGT 15 0.0072119194 52.000465 29 CGGTAGA 15 0.0072119194 52.000465 31 GGCGAGT 15 0.0072119194 52.000465 29 >>END_MODULE