FastQCFastQC Report
Tue 31 May 2016
SRR212849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223820
Sequences flagged as poor quality0
Sequence length58
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39301.7558752569028682No Hit
TGATCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25781.1518184255205075No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12160.5432937181663837No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT8540.3815566079885622No Hit
TGATCAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8520.38066303279420965No Hit
TGATCAGGGGCAGCAGAGCCCCAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAG6320.2823697614154231No Hit
TGATCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4900.21892592261638816No Hit
TGATCAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG4150.1854168528281655No Hit
TGATCAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.17916182646769727No Hit
TGATCAGGGGAATAAATGCAGATGTAAACTGCAGTTCCTCCATACAACCAAACCCGGT3830.1711196497185238No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAGCAGTGG3670.16397104816370298No Hit
TGATCAGGGACAGCTACTTGCCCAGAGGCTTTGTAGCCTAGCTAGAGGGCCATGCCCA3460.15458850862300064No Hit
TGATCAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.15235457063711913No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2990.13358949155571442No Hit
TGATCAGGGATAGCATAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTT2870.12822804038959879No Hit
TGATCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2550.1139308372799571No Hit
TGATCAGGGATCTGACCTGCAGCACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAA2370.10588866053078366No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA2270.10142078455902064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.007014413452.3674968
GGGCGAC203.3240014E-452.3674937
GTCTAGC203.4176762E-452.07362744
TCGACTG150.007178670352.0619443
CGAACAT150.007185044752.0502642
GCTTGCA307.9129677E-752.0502640
CTGTACG203.4290564E-452.0385852
CGTAACG150.007223378451.98028629
ATCCGTA150.00722978251.96863626
AATAGGG150.00722978251.96863624
AGTCCTA150.0072361951.95737
CTCAACG150.0072361951.95736
ACCCCGT150.0072361951.95733
CGTTTAT203.455728E-451.95699737
CAATCGG401.904482E-951.93373530
CTAAGCA150.00726829351.8988831
AGTATAG251.6744314E-551.87566811
GGTACAT203.4864116E-451.86407522
ATAGGAG150.00730050251.84089715
TAGCGTT150.00730050251.84089717
Produced by FastQC (version 0.11.5)